46 results about "Sperms viability" patented technology

The invention relates to preserving liquid for sperm, in particular to the preserving liquid for swine sperm, and belongs to the technical field of animal reproduction. The preserving liquid for the swine sperm comprises the following components: 25.5 to 35.0g/l of anhydrous dextrose, 5.1 to 7.0 g/l of trisodium citrate, 1 to 10 g/l of sodium bicarbonate, 2 to 4g/l of EDTA, 1 to 3g/l of potassium chloride, 2.4 to 3.4g/l of citric acid, 8.5 to 9.5g/l of trihydroxymethylaminomethane, 0.26 to 1g/l of amikacin and 0.2 to 1g/l of vitamin C. Through detection, in the swine sperm preserved by the preserving liquid, the sperm viability reaches 0.60 to 0.85 and the integrity rate of sperm acrosome reaches 90 percent, which excess the requirement of the current artificial fertilization.

The invention relates to a special clinical nutritional formula for male sterility. The special clinical nutritional formula is prepared from 15-40 parts of protein, 2-8 parts of fat, 40-55 parts of carbohydrates, 5.8-8 parts of dietary fibers, 0.6-1.2 parts of major elements, 0.01-0.04 part of minor elements, 0.005-0.02 part of fat-soluble vitamins, 0.008-0.2 part of water-soluble vitamins, 0.05-1 part of dietary essence, 0.1-2 parts of medicine-food homologous ingredient, 0.1-3 parts of natural plant compound and 0.005-5 parts of new resource food, wherein the medicine-food homologous ingredient is at least one selected from poria cocos, red raspberries, lycium barbarum, dioscorea opposita, wild jujube seeds, oysters and radix glycyrrhizae; all the ingredients are prepared into powder in advance, and the fat and the vitamins are prepared into powder by adopting a microcapsule coating technology. Through screening and proportioning the ingredients, the nutritional formula provided by the invention provides a more reasonable and more comprehensive nutritional support for male sterility sufferers so as to improve potential and occurred malnutrition state; meanwhile, kidney tonifying, spleen invigorating and liver soothing ingredients are added, so that the body condition of the male sterility sufferers is improved, the sperm production capability is promoted, and the sperm viability is improved.

A natural vegetable feed additive for stud ram relates to a feed additive for stud ram, which solves the problems of body situation reducing, libido and semen quality reducing of the ram during the service period, not high conception rate of ewe and service life reducing of the ram during the service period. The invention employs the prescription of the following components, which namely consists of Epimedium, dodder, actinolite, campanumaea pilosula, Rehmannia Glutinosa and Charred Triplet. The product of the invention enhances the sexual behavior of the ram, and remarkably improves the indexes like the sperm viability, sperm density, and ejaculatory amount, does not have side effects to the cloud characteristic of the sperm, the pH, the sperm abnormal rate, and the abnormal rate of acrosome, and can simultaneously prolong the service life of the stud ram by 1 to 1.5 years.

The invention discloses a trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof. The trace sperm cryopreservation carrier is in a rectangular, square or circular horizontal or groove shape and is made of a transparent plastic or transparent glass, and the size of the trace sperm cryopreservation carrier can bear 1-3 0.1-10.0 mu L freezing liquid micro drops. The freezing and thawing method comprises the following steps: after preparing the freezing liquid micro drops on the trace sperm cryopreservation carrier, inserting the trace sperm cryopreservation carrier into a sperm culture dish covered with oil, grabbing sperms by a microinjection needle, putting into the freezing liquid micro drops, taking out the cryopreservation carrier, soaking up mineral oil by virtue of an absorbent paper, rapidly freezing, directly inserting the cryopreservation carrier into an ICSI vessel when thawing, and thawing the sperms by utilizing the mineral oil with the temperature of 37 DEG C. According to the trace sperm cryopreservation carrier and the corresponding freezing and thawing method thereof disclosed by the invention, as the cryopreservation carrier is inserted into the sperm culture dish covered with oil, and the sperms are grabbed and frozen, a special moisture retention device is not needed, and evaporation of the freezing liquid micro drops is not needed to be worried about; the operation is very simple, the flexibility and reliability of assisted reproductive treatment for a patient with azoospermia are greatly improved, the sperm recovery rate can reach 100 percent, and the sperm viability is about 60 percent.

The invention relates to the field of livestock food, in particular to breeder cattle feed and a preparation method thereof. The breeder cattle feed is prepared from raw materials in parts by weight as follows: 50-70 parts of corn, 50-75 parts of bran, 30-50 parts of straw, 11-16 parts of lotus roots, 25-35 parts of carrots, 18-37 parts of bone meal, 15-25 parts of soybean cakes, 2-5 parts of radix codonopsis, 1-3 parts of herba epimedii, 5-7 parts of Chinese chives, 1-3 parts of Eucommia ulmoides, 2-4 parts of sesame oil, 1.1-1.8 parts of vitamins, 0.8-3 parts of microelements and 0.1-0.8 parts of aluminium hydroxide. The breeder cattle feed has the characteristics of comprehensive nutrition and good absorptivity, appetite of breeder cattle can be effectively improved, physical fitness and disease resistance of the breeder cattle are enhanced, seminal fluid quality of the breeder cattle is improved, furthermore, sperm viability and density are effectively improved, breeding rate is effectively increased, meanwhile, culturing cost is reduced, and earnings are increased.

The invention relates to a method for cryopreservation of donkey semen. The method comprises (1) preparing a base solution from 3.04 parts by mass of Tris, 1.70 parts by mass of citric acid, 1.50 parts by mass of glucose, 1.50 parts by mass of fructose, 0.06 parts by mass of penicillin, 0.10 parts by mass of streptomycin and 100 parts by mass of double distilled water and preparing a semen diluent from 85 parts by mass of the base solution and 15 parts by mass of an egg yolk solution, (2) using a rutting mare as a platform ass and collecting semen of a stud ass through a false vaginal method, wherein a motility rate is greater than 0.70, (3) taking the semen diluent and semen at 28 DEG C and carrying out dilution according to a ratio of 2: 1, (4) putting the semen diluent into a centrifuge tube, putting the centrifuge tube in a water bath at 28 DEG C and transferring the centrifuge tube into a refrigerator at 4 DEG C, and (5) in cryopreservation, carrying out uniform mixing every 24 hours and simultaneously detecting sperm viability through a microscope. The method obviously prolongs semen storage time, solves the problem of a large amount of artificial insemination in mare concentrated rutting in a large-scale donkey field, and effectively improves the utilization efficiency of the stud ass.

The invention discloses a compound feed for breeding boars. The compound feed comprises the following components in percentage by weight: 60-66% of corn flour, 3-5% of fish meal, 4-7% of fermented soybean meal, 10-15% of soybean meal, 10-11% of bran, 0.3% of multiple minerals, 0.5% of table salt, 0.04% of multiple vitamins, 0.12% of choline, 1.2% of dicalcium phosphate, 0.02% of phytase, 1.2% of stone powder, 0.12% of lysine, 0.03% of a probiotics compound and 0.5-1.0% of a Chinese herbal medicine compound for producing sperm. The compound feed disclosed by the invention is reasonable in formula design, provides comprehensive balanced nutrition for breeding boars, and creatively provides the Chinese herbal medicine compound for producing sperm, so that difficult problems that the semen quality is reduced and the sexual desire of the boars is poor caused by large sperm collection intensity of the breeding boars are solved; and the Chinese herbal medicine compound for producing sperm has the effects of nourishing the liver, nourishing the kidney, benefitting sperm, replenishing blood, reinforcing the spleen, invigorating the stomach and the like, so that after the breeding boars eat the compound feed, the sexual desire of the breeding boars is strengthened, the sperm viability, the sperm density and the semen volume are obviously improved, and the sperm abnormality rate is obviously reduced.

The invention relates to a pomfret sperm cryopreservation method including the steps as follows: selecting sexually matured parental pomfrets to gather fine-quality sperm; selecting suitable base liquid and anti-freezing liquid; mixing the sperm with the anti-freezing liquid by the volume ratio of 1:1 and then preserving the mixture on ice for 4-5 min; after reducing the temperature to minus 150 DEG C according to a three-step temperature reduction method, staying for 5 min; directly placing the mixture in liquid nitrogen for preservation; in defreezing, staying a freeze pipe in liquid nitrogen steam for 2 min, rapidly placing the freeze pipe in water bath of 28 DEG C to defreeze for 90 s and then placing the freeze pipe at room temperature to dissolve completely; and activating the defrozen sperm with sea water, wherein the rapid swimming time of the sperm is 57.41 s averagely, and the sperm viability (percent of the sperms moving rapidly accounting for all sperms) is 83.62% averagely. The fertilization rate of the pomfrets is greatly enhanced.

The invention relates to a Chinese herbal medicine nutritional feed for cultivating giant salamanders. The Chinese herbal medicine nutritional feed is prepared from the following components in parts by weight: 9-17 parts of astragalus membranaceus, 3-6 parts of bighead atractylodes rhizome, 3-5 parts of divaricate saposhnikovia root, 5-9 parts of semen cuscutae, 4-6 parts of epimedium herb, 4-8 parts of prepared rehmannia root, 0.3-0.6 part of calcium hydrogen phosphate, 2-4 parts of algae powder, 1-2 parts of unsaturated fatty acid, 1-2 parts of bacillus subtilis, 0.3-0.6 part of L-lysine hydrochloride, 0.05-0.1 part of zinc citrate, 0.05-0.1 part of organic iron, 0.1 part of vitamin and 0.2 part of microelement. The vitamin comprises VA, VB1, VB2, VB6, VB12, VC, VD and VE. The microelement is microelement amino acid chelate or is one, two or three of zinc, iron and calcium. The Chinese herbal medicine nutritional feed for cultivating the giant salamanders can meet the nutritional requirements of the giant salamanders, the immunity and the sexual gland maturity in mating seasons of the giant salamanders can be improved, and the sperm viability of male giant salamanders is enhanced.

The invention provides a conductive condom and a contraception device. The conductive condom comprises an electric conductor. The contraception device can emit an adjustable pulse electric signal. The conductive condom can conduct electricity and generate heat when the conductive condom is connected with the external electric signal. A key module of the contraception device is used for starting and stopping a whole intelligent device and setting the amplitudes, the widths and the delay times of pulse signals emitted by a pulse generation module. The pulse generation module adjusts the amplitudes, the widths and the delay times according to the setting of the key module under the control of a central processing unit. The electric signals generated by the pulse generation module are transmitted to a condom conductive set through a lantern ring conductive set. The pulse signals with different amplitudes, widths and delay times carry different sorts of energy, the sperm viability can be reduced to different extents, the capacity of sperms to be combined with ova is weakened, and accordingly the success rate of contraception is increased; the vaginas of females can be irritated to contract regularly, injured pelvic floor nerve muscles can be aroused, the muscle strength and the elasticity of the pelvic floor nerve muscles are improved, and accordingly vaginal relaxation of females is treated.

A method of assessing sperm viability by measuring the ability of the sperm to reduce a tetrazolium dye and allowing color development over a range of weakly acidic pHs.

The invention discloses a Chinese traditional medicine for curing sterility, which is made by mixing the following materials by weight: 20 to 40g of radix rehmanniae preparatas, 10 to 30g of radix salviae miltiorrhizae, 10 to 30g of angelicas, 10 to 30g of paeonia lactiflora pallas, 10 to 30g of cornels, 10 to 30g of red ginsengs, 10 to 30g of fructus cnidiis, 10 to 30g of raspberries, 10 to 30g of deerhorn glues, 10 to 30g of glue of tortoise plastrons, 10 to 30g of nutgrass galingale rhizomes, 10 to 30g of dried human placentas and 10 to 30g of amomum fruits. The invention is used by oral taking after being decocted to be soup or made into powder. The Chinese traditional medicine formed by the invention has the effects of benefiting vital energy, nourishing the blood, warming and invigorating the kidney essence, coordinating Chong and Conception vessels; moreover, the invention can increase sexual function for men, and improve sperm viability and sperm quantity, thereby women can getconceived; at the same time, the Chinese traditional medicine can adjust menstruation and ovulation state for men, improve impregnation condition, thereby achieving the effect of impregnation. Therefore, the invention is suitable for curing male-female sterility.

The invention provides a hybrid boar fodder and a preparation method thereof and relates to the technical field of poultry feeding. The hybrid boar fodder comprises a primary material, an auxiliary material and a fodder additive, wherein the primary material comprises corn, fish meal, bean pulp, egg powder, a biological fermentation fodder and wheat bran; the auxiliary material comprises phytase, an antioxidant, a mildew preventive, mildew decomposer, choline, sodium bicarbonate, multi-vitamins, compound organic microelement, calcium hydrophosphate, mountain flour and salt; and the fodder additive comprises coconut oil, soya-bean oil and a healthcare agent. After the hybrid boar is fed with the fodder, the quality of the seminal fluid of the hybrid boar can be increased; the technical problems of low yield and poor reproductive capacity of the hybrid boar caused by less seminal fluid, low sperm viability and teratospermia of the hybrid boar can be solved; and the technical effects of increasing the semen quality and increasing the conception rate of the hybrid boar can be achieved.

The invention belongs to the technical field of foods and functional foods, and provides a protein composition capable of improving sperm quality and sperm viability and a preparation method so as tosolve the technical problem that the sperm quality and the sperm viability cannot be effectively improved in the prior art. The composition comprises the following raw materials in parts by weight of0.5-50 parts of vegetable protein, 0.1-30 parts of an oyster extract, 5-30 parts of soluble dietary fibers, 0.5-20 parts of gamma-aminobutyric acids, 0.001-5 parts of organic selenium and 0.001-5 parts of organic zinc. The preparation method disclosed by the invention comprises the following step of uniformly mixing the vegetable protein with the oyster extract, the soluble dietary fibers, the gamma-aminobutyric acids, the organic selenium and the organic zinc in a weight ratio by a dry method, so as to obtain the protein composition. The protein composition disclosed by the invention has thecharacteristics of being delicately fragrant in flavor, fine and smooth in mouth feel, and capable of reducing the deformity rate of sperm heads, increasing the number of sperms and improving the sperm viability.

The invention discloses a medicine for treating urinary tract infection and a preparation method thereof, and belongs to the field of traditional Chinese medicines. Effective components of the medicine comprise the following raw materials: leaf of Chinese toona, common cephalanoplos herb, ammannia auriculata, leaf of shrubby baeckea, ludwigia prostrate, manyinflorescaned sweetvetch root, root of toothed habenaria, fruit of greywhitehair raspberry, herb of umbellate hawkweed, herb of clavateleaf oberonia, herb of smallflower beggarticks, clematoclethra argentifolia, bottle brush herb, root of Chinese clovershrub, root of wintersweet and stem of moupin dutchmanspipe. Medicinal materials adopted by the medicine disclosed by the invention have appropriate compatibility and can complement each other, so that the medicine has the effects of clearing heat and diminishing swelling, inducing diuresis and treating stranguria, nourishing the kidneys and invigorating the lung, eliminating dampness and removing toxicity, dispelling wind and relieving pain, and regulating qi and activating blood; various medicines can be combined to directly reach lesion sites so as to ensure that the medicine has the advantages of quick action, high cure rate, no toxic or side effects, safety and convenience in treating symptoms such as seminal vesicle enlargement, body pain, high blood pressure, repeatedly-unhealed infections, urine ammonia smell, seminal vesicle shrinkage, low sperm viability, painless scrotal mass and low sperm survival rate caused by urinary tract infection; and moreover, the medicine is cheap in price and easy to prepare, and can be widely applied to clinical treatment of urinary tract infection.

The invention belongs to the technical field of artificial insemination of pigs, and more specifically relates to a pig sperm diluent and application thereof. The pig sperm diluent is characterized inthat glucose is used as a nutritional agent; sodium citrate hydrate, dipotassium EDTA (Ethylene Diamine Tetraacetate) and anhydrous sodium bicarbonate are used as buffer agents; trehalose is used asa protective agent; berberine is used as an antibacterial agent; naringin is used as an antioxidant, and by utilizing stronger anti-radical action of the antioxidant, sperms are prevented from being subjected to peroxidation damage, and sperm plasma membranes are protected. By adopting the pig sperm diluent disclosed by the invention, the sperm viability is detected to be up to 85 percent after pig sperms are preserved for three days, and the sperm viability still can be enabled to be up to 75 percent after the pig sperms are preserved for seven days.