46 results about "Sperms viability" patented technology

Cryopreserved boar spermatozoa is much less fertile than that of other species, which could be due to damage to sperm membrane lipids during the cryopreservation process. Incorporation of selected lipids improves the survival of boar spermatozoa following cryopreservation.Liposomes were made from lipids extracted from head plasma membrane (HPM) of boar spematozoa or from selected lipids (SL) which contained specific phospholipids. At a fixed lipid concentration, fusion efficiency with spermatozoa as measured by flow cytometry and R18 dequenching was affected by lipid type, sperm concentration and incubation time.SL and HPM improved sperm viability (SYBR-14 and propidium iodide) and motility during cooling to 5C., with SL±egg yolk better than or equal to HPM (P<0.05). Post-thaw, egg yolk showed a strong cryoprotective effect. Compared to HPM, SL-treated sperm had higher post-thaw viability, progressive motility and total motility in the extender including egg yolk and higher viability in the extender excluding egg yolk (P<0.05).

The invention relates to preserving liquid for sperm, in particular to the preserving liquid for swine sperm, and belongs to the technical field of animal reproduction. The preserving liquid for the swine sperm comprises the following components: 25.5 to 35.0g / l of anhydrous dextrose, 5.1 to 7.0 g / l of trisodium citrate, 1 to 10 g / l of sodium bicarbonate, 2 to 4g / l of EDTA, 1 to 3g / l of potassium chloride, 2.4 to 3.4g / l of citric acid, 8.5 to 9.5g / l of trihydroxymethylaminomethane, 0.26 to 1g / l of amikacin and 0.2 to 1g / l of vitamin C. Through detection, in the swine sperm preserved by the preserving liquid, the sperm viability reaches 0.60 to 0.85 and the integrity rate of sperm acrosome reaches 90 percent, which excess the requirement of the current artificial fertilization.

The invention belongs to the technical field of screening of molecular markers of boars, and particularly relates to a molecular marker relative to sperm viability and a total sperm number of a boar and application. The molecular marker is cloned from a gene segment with a login number of ALGA 0001958. Through a gene chip technology, a gene is typed, screening is conducted to obtain the molecularmarker relative to characters of the sperm viability and the total sperm number of the boar, a marked nucleotide sequence is as shown in SEQ ID NO:1, A / G allele mutation exists in a base position of asite 101 of the sequence, and when nucleotide of the site 101 on the nucleotide sequence as shown in the SEQ ID NO:1 is A, the fact that the boar has higher sperm viability and a larger total sperm number is judged. The invention discloses a screening method of a molecular marker relative to a boar seminal fluid character and correlation analysis thereof. New SNP marker resources are provided formarker assisted selection of the characters of the sperm viability and the total sperm number of the boars.

The invention relates to a special clinical nutritional formula for male sterility. The special clinical nutritional formula is prepared from 15-40 parts of protein, 2-8 parts of fat, 40-55 parts of carbohydrates, 5.8-8 parts of dietary fibers, 0.6-1.2 parts of major elements, 0.01-0.04 part of minor elements, 0.005-0.02 part of fat-soluble vitamins, 0.008-0.2 part of water-soluble vitamins, 0.05-1 part of dietary essence, 0.1-2 parts of medicine-food homologous ingredient, 0.1-3 parts of natural plant compound and 0.005-5 parts of new resource food, wherein the medicine-food homologous ingredient is at least one selected from poria cocos, red raspberries, lycium barbarum, dioscorea opposita, wild jujube seeds, oysters and radix glycyrrhizae; all the ingredients are prepared into powder in advance, and the fat and the vitamins are prepared into powder by adopting a microcapsule coating technology. Through screening and proportioning the ingredients, the nutritional formula provided by the invention provides a more reasonable and more comprehensive nutritional support for male sterility sufferers so as to improve potential and occurred malnutrition state; meanwhile, kidney tonifying, spleen invigorating and liver soothing ingredients are added, so that the body condition of the male sterility sufferers is improved, the sperm production capability is promoted, and the sperm viability is improved.

A natural vegetable feed additive for stud ram relates to a feed additive for stud ram, which solves the problems of body situation reducing, libido and semen quality reducing of the ram during the service period, not high conception rate of ewe and service life reducing of the ram during the service period. The invention employs the prescription of the following components, which namely consists of Epimedium, dodder, actinolite, campanumaea pilosula, Rehmannia Glutinosa and Charred Triplet. The product of the invention enhances the sexual behavior of the ram, and remarkably improves the indexes like the sperm viability, sperm density, and ejaculatory amount, does not have side effects to the cloud characteristic of the sperm, the pH, the sperm abnormal rate, and the abnormal rate of acrosome, and can simultaneously prolong the service life of the stud ram by 1 to 1.5 years.

The invention discloses a trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof. The trace sperm cryopreservation carrier is in a rectangular, square or circular horizontal or groove shape and is made of a transparent plastic or transparent glass, and the size of the trace sperm cryopreservation carrier can bear 1-3 0.1-10.0 mu L freezing liquid micro drops. The freezing and thawing method comprises the following steps: after preparing the freezing liquid micro drops on the trace sperm cryopreservation carrier, inserting the trace sperm cryopreservation carrier into a sperm culture dish covered with oil, grabbing sperms by a microinjection needle, putting into the freezing liquid micro drops, taking out the cryopreservation carrier, soaking up mineral oil by virtue of an absorbent paper, rapidly freezing, directly inserting the cryopreservation carrier into an ICSI vessel when thawing, and thawing the sperms by utilizing the mineral oil with the temperature of 37 DEG C. According to the trace sperm cryopreservation carrier and the corresponding freezing and thawing method thereof disclosed by the invention, as the cryopreservation carrier is inserted into the sperm culture dish covered with oil, and the sperms are grabbed and frozen, a special moisture retention device is not needed, and evaporation of the freezing liquid micro drops is not needed to be worried about; the operation is very simple, the flexibility and reliability of assisted reproductive treatment for a patient with azoospermia are greatly improved, the sperm recovery rate can reach 100 percent, and the sperm viability is about 60 percent.

The invention relates to the technical field of feed, in particular to a functional composite dietary fiber, a preparation method and application. The functional composite dietary fiber provided by the invention is prepared from composite dietary fiber, composite grease, composite lactic acid bacteria and carriers. Wheat bran in the conventional feed can be replaced; through a cooperated matching effect, the intestinal environment can be improved; the intestinal tract movement can be promoted; the feed utilization rate is improved; the intestine lubrication and bowel relaxing effects can be achieved; meanwhile, the production performance of the functional composite dietary fiber can be enhanced. The functional composite dietary fiber provided by the invention can be added to pig feed of stock boars, replacement sows, pregnant sows and lactation sows; the sperm viability of the boars can be enhanced; the constipation rate of sows is reduced; the milk yield is improved; the piglet survival rate, the piglet body weight and the like are improved.

The invention discloses an anti-heat stress feed additive for improving reproductive function of male rabbits. The anti-heat stress feed additive is added to a basal diet feed prepared for maintaining the right amount of recommended NRC (National Research Council) (1977) adult rabbits for uniformly mixing. The anti-heat stress feed additive added to a ton of feed comprises the following components by weight: 100 to 800g of lipoic acid, 100 to 600g of vitamin E, 100 to 600g of vitamin C and 0 to 600g of Saccharicterpenin. A result shows that the additive can obviously improve sperm viability and density and reduce abnormal rate (P<0.05), wherein anti-stress test 3 has the advantage of improving sperm viability; anti-stress test 2 has the advantage of improving sperm density; and all of the three additives can obviously improve the content (P<0.05) of testosterone in serum of heat stress bucks and do not have obvious difference (P>0.05) for improving the content of T3 and cortisol.

The present invention relates to the technical field of culture and particularly discloses a simple method for comprehensively evaluating the quality of boar semen. The method comprises the following steps of: firstly, detecting four indexes, namely sperm density, semen quantity, sperm viability and malformation rate; secondly, calculating the total number of active sperms according to a calculation formula; and thirdly, evaluating the quality of the boar semen according to the total number of the active sperms. According to the method, the quality of the boar semen can be objectively and accurately evaluated.

The invention relates to the field of livestock food, in particular to breeder cattle feed and a preparation method thereof. The breeder cattle feed is prepared from raw materials in parts by weight as follows: 50-70 parts of corn, 50-75 parts of bran, 30-50 parts of straw, 11-16 parts of lotus roots, 25-35 parts of carrots, 18-37 parts of bone meal, 15-25 parts of soybean cakes, 2-5 parts of radix codonopsis, 1-3 parts of herba epimedii, 5-7 parts of Chinese chives, 1-3 parts of Eucommia ulmoides, 2-4 parts of sesame oil, 1.1-1.8 parts of vitamins, 0.8-3 parts of microelements and 0.1-0.8 parts of aluminium hydroxide. The breeder cattle feed has the characteristics of comprehensive nutrition and good absorptivity, appetite of breeder cattle can be effectively improved, physical fitness and disease resistance of the breeder cattle are enhanced, seminal fluid quality of the breeder cattle is improved, furthermore, sperm viability and density are effectively improved, breeding rate is effectively increased, meanwhile, culturing cost is reduced, and earnings are increased.

The invention relates to a method for cryopreservation of donkey semen. The method comprises (1) preparing a base solution from 3.04 parts by mass of Tris, 1.70 parts by mass of citric acid, 1.50 parts by mass of glucose, 1.50 parts by mass of fructose, 0.06 parts by mass of penicillin, 0.10 parts by mass of streptomycin and 100 parts by mass of double distilled water and preparing a semen diluent from 85 parts by mass of the base solution and 15 parts by mass of an egg yolk solution, (2) using a rutting mare as a platform ass and collecting semen of a stud ass through a false vaginal method, wherein a motility rate is greater than 0.70, (3) taking the semen diluent and semen at 28 DEG C and carrying out dilution according to a ratio of 2: 1, (4) putting the semen diluent into a centrifuge tube, putting the centrifuge tube in a water bath at 28 DEG C and transferring the centrifuge tube into a refrigerator at 4 DEG C, and (5) in cryopreservation, carrying out uniform mixing every 24 hours and simultaneously detecting sperm viability through a microscope. The method obviously prolongs semen storage time, solves the problem of a large amount of artificial insemination in mare concentrated rutting in a large-scale donkey field, and effectively improves the utilization efficiency of the stud ass.

The invention discloses a compound feed for breeding boars. The compound feed comprises the following components in percentage by weight: 60-66% of corn flour, 3-5% of fish meal, 4-7% of fermented soybean meal, 10-15% of soybean meal, 10-11% of bran, 0.3% of multiple minerals, 0.5% of table salt, 0.04% of multiple vitamins, 0.12% of choline, 1.2% of dicalcium phosphate, 0.02% of phytase, 1.2% of stone powder, 0.12% of lysine, 0.03% of a probiotics compound and 0.5-1.0% of a Chinese herbal medicine compound for producing sperm. The compound feed disclosed by the invention is reasonable in formula design, provides comprehensive balanced nutrition for breeding boars, and creatively provides the Chinese herbal medicine compound for producing sperm, so that difficult problems that the semen quality is reduced and the sexual desire of the boars is poor caused by large sperm collection intensity of the breeding boars are solved; and the Chinese herbal medicine compound for producing sperm has the effects of nourishing the liver, nourishing the kidney, benefitting sperm, replenishing blood, reinforcing the spleen, invigorating the stomach and the like, so that after the breeding boars eat the compound feed, the sexual desire of the breeding boars is strengthened, the sperm viability, the sperm density and the semen volume are obviously improved, and the sperm abnormality rate is obviously reduced.

The invention relates to a pomfret sperm cryopreservation method including the steps as follows: selecting sexually matured parental pomfrets to gather fine-quality sperm; selecting suitable base liquid and anti-freezing liquid; mixing the sperm with the anti-freezing liquid by the volume ratio of 1:1 and then preserving the mixture on ice for 4-5 min; after reducing the temperature to minus 150 DEG C according to a three-step temperature reduction method, staying for 5 min; directly placing the mixture in liquid nitrogen for preservation; in defreezing, staying a freeze pipe in liquid nitrogen steam for 2 min, rapidly placing the freeze pipe in water bath of 28 DEG C to defreeze for 90 s and then placing the freeze pipe at room temperature to dissolve completely; and activating the defrozen sperm with sea water, wherein the rapid swimming time of the sperm is 57.41 s averagely, and the sperm viability (percent of the sperms moving rapidly accounting for all sperms) is 83.62% averagely. The fertilization rate of the pomfrets is greatly enhanced.

The invention discloses sperm piRNA (Piwi-interacting Ribonucleic Acid) marker combination related to male reproductive functional disturbance and application thereof. A marker comprises ten piRNAs (mainly comprising piR-1207 and piR-2107), and the sperm piRNA marker combination can be used for detection and prediction of sperm viability. In the invention, sperm can be relatively easily obtained, other tissues are not required, and the detection belongs to non-traumatic examination; sperm piRNA and MitoPLD (Phospholipase D) protein can be used for reflecting pathological and physiological status of a whole spermatogenic process at a molecular level, the accurate level of detection can be increased by taking the sperm piRNA and the MitoPLD protein as auxiliary detection indexes, and a potential target spot is provided for treatment of the male reproductive functional disturbance.

The invention relates to a Chinese herbal medicine nutritional feed for cultivating giant salamanders. The Chinese herbal medicine nutritional feed is prepared from the following components in parts by weight: 9-17 parts of astragalus membranaceus, 3-6 parts of bighead atractylodes rhizome, 3-5 parts of divaricate saposhnikovia root, 5-9 parts of semen cuscutae, 4-6 parts of epimedium herb, 4-8 parts of prepared rehmannia root, 0.3-0.6 part of calcium hydrogen phosphate, 2-4 parts of algae powder, 1-2 parts of unsaturated fatty acid, 1-2 parts of bacillus subtilis, 0.3-0.6 part of L-lysine hydrochloride, 0.05-0.1 part of zinc citrate, 0.05-0.1 part of organic iron, 0.1 part of vitamin and 0.2 part of microelement. The vitamin comprises VA, VB1, VB2, VB6, VB12, VC, VD and VE. The microelement is microelement amino acid chelate or is one, two or three of zinc, iron and calcium. The Chinese herbal medicine nutritional feed for cultivating the giant salamanders can meet the nutritional requirements of the giant salamanders, the immunity and the sexual gland maturity in mating seasons of the giant salamanders can be improved, and the sperm viability of male giant salamanders is enhanced.

The invention provides a conductive condom and a contraception device. The conductive condom comprises an electric conductor. The contraception device can emit an adjustable pulse electric signal. The conductive condom can conduct electricity and generate heat when the conductive condom is connected with the external electric signal. A key module of the contraception device is used for starting and stopping a whole intelligent device and setting the amplitudes, the widths and the delay times of pulse signals emitted by a pulse generation module. The pulse generation module adjusts the amplitudes, the widths and the delay times according to the setting of the key module under the control of a central processing unit. The electric signals generated by the pulse generation module are transmitted to a condom conductive set through a lantern ring conductive set. The pulse signals with different amplitudes, widths and delay times carry different sorts of energy, the sperm viability can be reduced to different extents, the capacity of sperms to be combined with ova is weakened, and accordingly the success rate of contraception is increased; the vaginas of females can be irritated to contract regularly, injured pelvic floor nerve muscles can be aroused, the muscle strength and the elasticity of the pelvic floor nerve muscles are improved, and accordingly vaginal relaxation of females is treated.

The invention relates to a diluent for preserving rabbit sperms at normal temperature and a preparation method thereof and belongs to the technical field of animal reproduction. The diluent is prepared by adding water into 30-40g of tris(hydroxymethyl)aminomethane, 15-20g of citric acid, 6-15g of dextrose, 0.5-1g of potassium chloride, 0.2-0.5g of glutathione and 0.05-0.1g of kanamycin until the total volume reaches 1L; and the pH value of the diluent is 6.8-7.0. By using the diluent, the rabbit sperms can be preserved for a short time, and the sperm viability can be 0.7 and the acrosome integrity rate can be 87% after the rabbit sperms are preserved by using the diluent for 48 hours at normal temperature, thus the sperm viability and the acrosome integrity rate exceed the requirements of artificial insemination of rabbits, the conception rate is up to 89.4%, and the conception rate reaches or is superior to those obtained by using the similar import diluents. Meanwhile, the diluent provided by the invention is used and stored more conveniently, and the diluent can be instant availably at the time of artificial insemination, thus being convenient and quick. The diluent is prepared simply and can be used for lowering the cost of the artificial insemination of the rabbits.

A method of assessing sperm viability by measuring the ability of the sperm to reduce a tetrazolium dye and allowing color development over a range of weakly acidic pHs.

In order to solve the technical problems, the invention provides a method for identifying sperm viability of a large yellow croaker, relates to fish sperm viability detection, and provides a kit and an ultraviolet spectrophotometer to collect sperm of the large yellow croaker. The method is characterized in that the kit detects the viability of related energy metabolism enzyme in sperm by using the ultraviolet spectrophotometer; in the sperm, if the viability of ATP enzyme is higher than 60.16+ / -5.88U / ml, the viability of CK is higher than 11.91+ / -0.76U / ml, the viability of SDH is higher than 51+ / -2.16U / ml, and the viability of LDH is higher than 7,806.44+ / -110.11U / L, the sperm has higher viability; and in the sperm, if the viability of the ATP enzyme is lower than 3.54+ / -0.37U / ml, the viability of the CK is lower than 10.22+ / -0.32U / ml, the viability of the SDH is lower than 31.5+ / -2.08U / ml, and the viability of the LDH is lower than 2,654.13+ / -70.06U / L, the sperm has low viability.

The invention discloses a Chinese traditional medicine for curing sterility, which is made by mixing the following materials by weight: 20 to 40g of radix rehmanniae preparatas, 10 to 30g of radix salviae miltiorrhizae, 10 to 30g of angelicas, 10 to 30g of paeonia lactiflora pallas, 10 to 30g of cornels, 10 to 30g of red ginsengs, 10 to 30g of fructus cnidiis, 10 to 30g of raspberries, 10 to 30g of deerhorn glues, 10 to 30g of glue of tortoise plastrons, 10 to 30g of nutgrass galingale rhizomes, 10 to 30g of dried human placentas and 10 to 30g of amomum fruits. The invention is used by oral taking after being decocted to be soup or made into powder. The Chinese traditional medicine formed by the invention has the effects of benefiting vital energy, nourishing the blood, warming and invigorating the kidney essence, coordinating Chong and Conception vessels; moreover, the invention can increase sexual function for men, and improve sperm viability and sperm quantity, thereby women can getconceived; at the same time, the Chinese traditional medicine can adjust menstruation and ovulation state for men, improve impregnation condition, thereby achieving the effect of impregnation. Therefore, the invention is suitable for curing male-female sterility.

The invention provides a hybrid boar fodder and a preparation method thereof and relates to the technical field of poultry feeding. The hybrid boar fodder comprises a primary material, an auxiliary material and a fodder additive, wherein the primary material comprises corn, fish meal, bean pulp, egg powder, a biological fermentation fodder and wheat bran; the auxiliary material comprises phytase, an antioxidant, a mildew preventive, mildew decomposer, choline, sodium bicarbonate, multi-vitamins, compound organic microelement, calcium hydrophosphate, mountain flour and salt; and the fodder additive comprises coconut oil, soya-bean oil and a healthcare agent. After the hybrid boar is fed with the fodder, the quality of the seminal fluid of the hybrid boar can be increased; the technical problems of low yield and poor reproductive capacity of the hybrid boar caused by less seminal fluid, low sperm viability and teratospermia of the hybrid boar can be solved; and the technical effects of increasing the semen quality and increasing the conception rate of the hybrid boar can be achieved.

The invention discloses sperm piRNA (Piwi-interacting Ribonucleic Acid) marker combination related to male reproductive functional disturbance and application thereof. A marker comprises ten piRNAs (mainly comprising piR-1207 and piR-2107), and the sperm piRNA marker combination can be used for detection and prediction of sperm viability. In the invention, sperm can be relatively easily obtained, other tissues are not required, and the detection belongs to non-traumatic examination; sperm piRNA and MitoPLD (Phospholipase D) protein can be used for reflecting pathological and physiological status of a whole spermatogenic process at a molecular level, the accurate level of detection can be increased by taking the sperm piRNA and the MitoPLD protein as auxiliary detection indexes, and a potential target spot is provided for treatment of the male reproductive functional disturbance.

The invention belongs to the technical field of foods and functional foods, and provides a protein composition capable of improving sperm quality and sperm viability and a preparation method so as tosolve the technical problem that the sperm quality and the sperm viability cannot be effectively improved in the prior art. The composition comprises the following raw materials in parts by weight of0.5-50 parts of vegetable protein, 0.1-30 parts of an oyster extract, 5-30 parts of soluble dietary fibers, 0.5-20 parts of gamma-aminobutyric acids, 0.001-5 parts of organic selenium and 0.001-5 parts of organic zinc. The preparation method disclosed by the invention comprises the following step of uniformly mixing the vegetable protein with the oyster extract, the soluble dietary fibers, the gamma-aminobutyric acids, the organic selenium and the organic zinc in a weight ratio by a dry method, so as to obtain the protein composition. The protein composition disclosed by the invention has thecharacteristics of being delicately fragrant in flavor, fine and smooth in mouth feel, and capable of reducing the deformity rate of sperm heads, increasing the number of sperms and improving the sperm viability.

The invention provides a medicine used for treating varicocele induced sterility. The medicine comprises the following active pharmaceutical ingredients: epimedium herb, Chinese ligusticum rhizome, barbary wolfberry fruit, Chinese dodder seed, malaytea scurfpea fruit, tamariskoid spikemoss herb, radix astragali, red paeony root, common anemarrhena rhizome, rhizoma dioscoreae, herb of incisory spleenwort, all-grass of inconspicuous bulbophyllum, Chinese taxillus twig, gordon euryale seed, fruit of largeflower gardenia, suberect spatholobus stem, root of entire micromelum, penis and testis of ox, partition in fruit of English walnut, root of trifoliate acanthopanax, herb of henbit deadnettle and morinda root. The medicine has the beneficial effects that being matched with operative treatment, the medicine can achieve the effects of further promoting blood circulation of testes, improving ischemic hypoxia and metabolism and excretion of toxic substances, promoting improvement of the function of spermatogenesis and the quality of semen and reinforcing the sperm viability.

The invention discloses a medicine for treating urinary tract infection and a preparation method thereof, and belongs to the field of traditional Chinese medicines. Effective components of the medicine comprise the following raw materials: leaf of Chinese toona, common cephalanoplos herb, ammannia auriculata, leaf of shrubby baeckea, ludwigia prostrate, manyinflorescaned sweetvetch root, root of toothed habenaria, fruit of greywhitehair raspberry, herb of umbellate hawkweed, herb of clavateleaf oberonia, herb of smallflower beggarticks, clematoclethra argentifolia, bottle brush herb, root of Chinese clovershrub, root of wintersweet and stem of moupin dutchmanspipe. Medicinal materials adopted by the medicine disclosed by the invention have appropriate compatibility and can complement each other, so that the medicine has the effects of clearing heat and diminishing swelling, inducing diuresis and treating stranguria, nourishing the kidneys and invigorating the lung, eliminating dampness and removing toxicity, dispelling wind and relieving pain, and regulating qi and activating blood; various medicines can be combined to directly reach lesion sites so as to ensure that the medicine has the advantages of quick action, high cure rate, no toxic or side effects, safety and convenience in treating symptoms such as seminal vesicle enlargement, body pain, high blood pressure, repeatedly-unhealed infections, urine ammonia smell, seminal vesicle shrinkage, low sperm viability, painless scrotal mass and low sperm survival rate caused by urinary tract infection; and moreover, the medicine is cheap in price and easy to prepare, and can be widely applied to clinical treatment of urinary tract infection.

The invention discloses a method for cryopreserving large yellow croaker sperm: through steps such as antifreeze preparation, sperm collection, preparation of a mixture of antifreeze and semen, subpackaging of the mixture and freezing of the mixture, the method is a convenient operation , the sperm survival rate is higher, the preservation quality of the sperm is better, and the activation rate of the sperm after the cryopreservation is relatively high. The large yellow croaker sperm cryopreservation method; There is liquid nitrogen built in the foam box, the depth of the foam box is 18 cm, and the wall thickness is 2.5 cm. On the two long walls of the foam box, grooves are symmetrically arranged from the top of the foam box downwards, and the grooves are formed from the foam box. The depth of the top of the box is 0.5 cm, and the distance between the liquid level of liquid nitrogen and the groove is 2.5 to 3.5 cm; the device is easy to operate, avoids the phenomenon of liquid nitrogen vapor covering after opening the cover, and saves freezing time , It also avoids the advantages of the straw falling into the liquid nitrogen due to rolling.

The invention relates to the field of animal reproduction, in particular to a method for separating sex-controlled semen, comprising the following steps: filtering raw semen through a 40-60 micron filter for the first time to obtain filtered semen from which sperm masses and impurities are removed; detecting the filtered semen; The viability of the semen is to obtain the semen to be dyed and filtered with a viability of 0.6-0.85; the semen to be dyed and filtered with a viability of 0.6-0.85 is dyed to obtain the dyed semen; the dyed semen is separated by a flow cytometer to obtain an X-chromosome-containing semen semen and semen containing Y chromosomes. The method for separating the sex-controlled semen provided by the invention can ensure the uniformity and stability of the semen dyeing after dyeing and filtration, thereby facilitating the efficient separation of the semen containing the X chromosome and the semen containing the Y chromosome. In addition, the filtered semen is less likely to block the nozzle of the flow cytometer, thereby ensuring the smooth progress of the entire separation process.

The invention belongs to the technical field of artificial insemination of pigs, and more specifically relates to a pig sperm diluent and application thereof. The pig sperm diluent is characterized inthat glucose is used as a nutritional agent; sodium citrate hydrate, dipotassium EDTA (Ethylene Diamine Tetraacetate) and anhydrous sodium bicarbonate are used as buffer agents; trehalose is used asa protective agent; berberine is used as an antibacterial agent; naringin is used as an antioxidant, and by utilizing stronger anti-radical action of the antioxidant, sperms are prevented from being subjected to peroxidation damage, and sperm plasma membranes are protected. By adopting the pig sperm diluent disclosed by the invention, the sperm viability is detected to be up to 85 percent after pig sperms are preserved for three days, and the sperm viability still can be enabled to be up to 75 percent after the pig sperms are preserved for seven days.