7 results about "Sperms viability" patented technology

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The present application relates to the field of animal reproduction technology, and in particular to a sperm protection agent for improving sperm preservation quality and embryo development potential and application thereof, wherein the effective components of the sperm protection agent are selected from any one or several of acetyl carnitine, glycerophosphocholine, tauroursodeoxycholic acid, glutamic acid, sunflower acylcarnitine, pyridoxal phosphate and glyco ursodeoxycholic acid. Based on the regulation of the OR4C13-G13 / Golf signal axis related to sperm capacitation of the effective components, sperm viability can be maintained during preservation, premature capacitation can be inhibited, and the fertilization rate and embryo development potential can be improved. The present application is suitable for the normal temperature preservation of pig semen and can be extended to other mammalian or human assisted reproduction fields.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.

The present invention discloses a method for assessing the activity of Takifugu sperm cryopreservation solution based on computer vision. [Solution] Takifugu sperm are cryopreserved for long periods using a cryopreservation solution, thawed, and then activated. After observation under a microscope, sperm motility videos are recorded. Data augmentation is performed on the video using a binarization method. Based on two deep learning algorithms for target detection and target tracking, the positional changes of multiple Takifugu sperm targets between consecutive frames are tracked and captured, and the significant movements and trajectories of the recognized sperm are analyzed for imitation training. The sperm's movement and viability are determined by statistically analyzing their displacement between different frames. Finally, a visualized sperm viability graph is generated. This method enables long-term cryopreservation of highly active Takifugu sperm, providing technical support for year-round reproduction and species resource conservation. It also improves the accuracy of Takifugu sperm activity detection, simplifies the detection and recognition process, and reduces costs.

The invention discloses a semen freezing liquid and its application. The semen freezing liquid of the invention comprises melatonin and N,N-dimethylformamide. The invention also discloses the application of melatonin in the cryopreservation of semen of poultry animals. The semen freezing liquid of the invention can significantly improve the indexes such as sperm motility rate, plasma membrane integrity, acrosome integrity, etc. after thawing, reduce the level of ROS in semen, improve the level of sperm mitochondrial membrane potential, and improve the hatching rate of artificial insemination of semen after thawing, and can be used in the fields of poultry semen cryopreservation, germplasm resource preservation and utilization.

The invention provides sperm diluting powder for maintaining sperm motility, and belongs to the technical field of animal breeding. Heat shock proteins HSP 90-alpha and HSPB1 are expressed through genetic engineering escherichia coli, are purified and are compounded with inorganic salt and the like to form a sperm diluent, and the sperm diluent is freeze-dried to prepare the sperm freeze-dried powder. The sperm dilution powder containing the heat shock protein HSP 90-alpha or the heat shock protein HSPB1 can reduce the injury of chicken sperms and pig sperms and maintain the sperm activity, and meanwhile, the sperm dilution powder containing the heat shock protein HSP 90-alpha and the heat shock protein HSPB1 has the advantages that the sperm activity maintaining effect is better than that of the sperm dilution powder containing the heat shock protein HSP 90-alpha and the heat shock protein HSPB1 under the synergistic effect compared with that of the sperm dilution powder containing the heat shock protein HSP 90-alpha and the heat shock protein HSPB1 containing the heat shock protein HSP 90-alpha and the heat shock protein HSPB1 containing the heat shock protein HSP 90-alpha.

The present application belongs to the field of ultra-low temperature cryopreservation technology, and in particular, relates to a cryopreservation tube and a method and application of cryopreservation of grouper. The bottom of the cryopreservation tube of the present application is concave upward to form a hollow tube that exceeds the top of the tube, and the two ends of the hollow tube are connected. Since there is no blockage at both ends of the hollow tube, the airflow is smooth, and the hollow tube also increases the surface area of ​​the hollow cryopreservation tube, thereby accelerating the cooling / heating rate, improving the uniformity of cooling / heating, and improving the freezing effect. The sperm motility rate after thawing is high, thereby solving the technical problem of poor sperm freezing effect of cryopreservation tubes in the prior art.