4 results about "Sperms viability" patented technology

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The present application relates to the field of animal reproduction technology, and in particular to a sperm protection agent for improving sperm preservation quality and embryo development potential and application thereof, wherein the effective components of the sperm protection agent are selected from any one or several of acetyl carnitine, glycerophosphocholine, tauroursodeoxycholic acid, glutamic acid, sunflower acylcarnitine, pyridoxal phosphate and glyco ursodeoxycholic acid. Based on the regulation of the OR4C13-G13 / Golf signal axis related to sperm capacitation of the effective components, sperm viability can be maintained during preservation, premature capacitation can be inhibited, and the fertilization rate and embryo development potential can be improved. The present application is suitable for the normal temperature preservation of pig semen and can be extended to other mammalian or human assisted reproduction fields.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.

The present invention discloses a method for assessing the activity of Takifugu sperm cryopreservation solution based on computer vision. [Solution] Takifugu sperm are cryopreserved for long periods using a cryopreservation solution, thawed, and then activated. After observation under a microscope, sperm motility videos are recorded. Data augmentation is performed on the video using a binarization method. Based on two deep learning algorithms for target detection and target tracking, the positional changes of multiple Takifugu sperm targets between consecutive frames are tracked and captured, and the significant movements and trajectories of the recognized sperm are analyzed for imitation training. The sperm's movement and viability are determined by statistically analyzing their displacement between different frames. Finally, a visualized sperm viability graph is generated. This method enables long-term cryopreservation of highly active Takifugu sperm, providing technical support for year-round reproduction and species resource conservation. It also improves the accuracy of Takifugu sperm activity detection, simplifies the detection and recognition process, and reduces costs.