The invention discloses an anther culture method for indica japonica hybrid rice. The method comprises the following steps: sampling, identifying a microspore development period, low-temperature pretreatment, young wheatear sterilization, anther inoculation, still dark cultivation at 25+/- 2 DEG C until the diameter of embryonic callus is 2-3mm; carrying out a redifferentiation stage to form complete regenerated plants with haploid or double haploids. The double haploids are good breeding materials, and can be directly used for breeding of new varieties or varietal improvement; the breeding age limit is greatly shortened; the selection efficiency is improved; the double haploids also are ideal materials for molecular markers, such as amplified fragment length polymorphism (AFLP), restricted fragment length polymorphisms (RFLP), randomly amplified polymorphic deoxyribonucleic acid (RAPD) and the like, genetic map drawing, and researches on animal evolution, genetic analysis, plant gene cloning and screening, genetic engineering breeding and the like.