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Method for identifying red feather-induced mutant genotype of white leghorns and breeding method for complete set line of red feather pink shell laying hens

A genotype and heterozygous genotype technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. To solve problems such as the influence of the size of the expansion group, it can speed up the breeding process, avoid cumbersome and reduce the breeding cost.

Active Publication Date: 2018-06-22
BEIJING HUADU YUKOU POULTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional breeding method adopts the method of test crossing, and screens the white legion chickens carrying the red feather mutation according to the feather color phenotype of the hybrid offspring. 100% accuracy cannot be achieved

Method used

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  • Method for identifying red feather-induced mutant genotype of white leghorns and breeding method for complete set line of red feather pink shell laying hens
  • Method for identifying red feather-induced mutant genotype of white leghorns and breeding method for complete set line of red feather pink shell laying hens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Establishment of primers and method for genotype identification of red feather causative mutation in Bailaihang chicken

[0050] 1. Discovery of causative mutation sites in red feathers of Bailaihang chicken

[0051] 1. Extract the genomic DNA of the chicken to be tested

[0052] Using 2000 Beijing Huadu Yukou Poultry Industry Co., Ltd. Bailaihang strain hens to cross with Luodao red roosters, the feather color phenotypes of the hybrid offspring were counted, and the number of offspring hens was more than 5 and 100% were red feathers. 9 aircraft carrier chickens were used as the red feather group, and 9 white aircraft carrier chickens whose offspring hens were greater than 5 and 100% white were selected as the white feather group; these 2 groups were used as chickens to be tested.

[0053] Blood was collected from the wing veins of the chickens to be tested, treated with anticoagulants, lysed and digested with protease, and then the genomic DNA was extracted...

Embodiment 2

[0119] Example 2. Breeding of red-feather causative mutation homozygous genotype White Legion female parent and establishment of matching line of red-feather powder-shell layer hens

[0120] 1. Breeding of female parent of Hongyu Causal Mutation Homozygous Genotype White Legion Chicken

[0121] 1. Genomic DNA extraction

[0122] Genomic DNA of 1573 Bailai aircraft carrier chickens to be tested was extracted.

[0123] 2. PCR amplification

[0124] Using the genomic DNA of 1573 Bailai aircraft carrier chickens to be tested as templates, PCR amplification was performed in step 2 of the above three methods in Example 1 to obtain PCR amplification products.

[0125] 3. Digestion

[0126] Nru I digest the PCR amplified product of each of the above-mentioned chickens to obtain the digested product.

[0127] Detect the size of the digested product:

[0128] If the size of the enzymatic digestion product of the Bailai aircraft carrier chicken to be tested is only 253bp, then the Ba...

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Abstract

The invention discloses a breeding method for a complete set line of red feather pink shell laying hens and provides a primer pair for identifying a red feather-induced mutant genotype of white leghorns. The primer pair consists of a single-stranded DNA molecule shown as a sequence 2 in a sequence table and a single-stranded DNA molecule shown as a sequence 3 in the sequence table. The primer pairis characterized in that primers are designed according to nucleotide sequences on the upstream and downstream of 18,288,303th deoxynucleotide on a positive-sense strand of an eleventh chromosome ina chicken reference genome Gallus_gallus-4.0 version sequence information disclosed by NCBI; a locus genotype is detected by restriction fragment length polymorphism, the white leghorns with homozygous red feather-induced mutant genotype are propagated, and pure line female parents are bred; hybrid hens obtained by female parents and Rhode Island Red male parents are completely in a red feather phenotype; the breeding method can meet market demand and has a broad popularization prospect.

Description

technical field [0001] The invention belongs to molecular biology and poultry breeding technology, and in particular relates to a genotype identification of red-feather causative mutation of white-legged chickens and a method for cultivating matching lines of red-feather powder-shell layer hens. Background technique [0002] China is a country with traditional gourmet food. Consumers' demand for eggs has obvious regional characteristics, so the demand for laying hen breeds is also diversified and regionalized. With the improvement of living standards, consumers' demand for eggs not only pays attention to the internal quality, but also pays attention to the size of eggs and the color of eggshells. The types of egg demand can be roughly divided into luxury consumption demand, high-end consumption demand, mass consumption demand and egg processing demand. The pink-shell layer breed has become the "new favorite" of residents' egg consumption due to the small egg weight and larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/683C12N15/11A01K67/02
CPCA01K67/02C12Q1/683C12Q1/6888C12Q2600/124C12Q2600/156C12Q1/6876C12N9/22C12Q1/6806C12Q2531/113
Inventor 吴桂琴杨宁孙皓李光奇孙从佼李花妮刘爱巧
Owner BEIJING HUADU YUKOU POULTRY
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