17 results about "Mutant genotype" patented technology

The invention discloses a molecular marker remarkably related to the amylose content of wheat and application of the molecular marker, and belongs to the technical field of wheat breeding. Three mutation sites of wheat starch branching enzyme gene SBEIIa on 2A, 2B and 2D chromosomes and two mutation sites of SBEIIb on 2A and 2B chromosomes are identified, a KASP molecular marker system closely linked with SBEIIa and / or SBEIIb is developed based on the SNP sites, the detection process is simple and convenient, the cost is low, the throughput is high, and the application prospect is wide. According to the method, the high-amylose wheat material which contains the mutant genotype and can be stably inherited can be accurately and efficiently screened out, the breeding process of wheat variety quality improvement can be accelerated, and the method has important significance on creation of the high-amylose wheat material or molecular assisted breeding of the high-amylose wheat.

The invention relates to the technical field of corn molecular breeding, in particular to a molecular marker, a primer pair and a kit aiming at an acquired shearing site of a corn dwarf gene Br2 and application of the molecular marker, the primer pair and the kit. An acquired cleavage site of a corn dwarf gene Br2 is located at a 206632482th basic group of a corn chromosome 1, a wild type of a nucleotide sequence of the molecular marker is shown as a sequence 1, a basic group of a variation site is G, it is indicated that a Br2 genotype of corn to be detected is a wild genotype, a basic group of the variation site is A, and it is indicated that the Br2 genotype of the corn to be detected is a homozygous mutation genotype; the plant height of the corn of which the genotype is the homozygous mutation genotype is lower than that of the wild genotype. The gene locus mutation provided by the invention can reduce the corn plant height, the molecular marker can be used for mutation locus detection, and accurate targets and genetic resources can be provided for corn plant type improvement in breeding production.

Embodiments of the present application provide a sample pollution detection method, device, system and related equipment, the method comprises: obtaining nucleic acid information of a sample; calculating the mutation genotype frequency value BAF of the sample at a preset site; wherein the preset site is multiple, and in a non-polluted sample, the proportion of homozygous sites in the preset site is greater than the first proportion; determining the homozygous site in the preset site according to the BAF of the preset site; calculating the proportion of the homozygous site in the preset site, if the proportion of the homozygous site is less than the first proportion, the sample is a contaminated sample. Embodiments of the present application can simply and directly judge whether the sample is contaminated.

Compositions, kits, and methods that implement neoantigens specific to cancer cells having recurrent mutations in SRSF2 or ZRSR2, wherein the mutations are shared across patients with those mutational genotypes, as well as cognate T cell receptors (TCRs) that specifically recognize those neoantigens.

The invention is applicable to the field of molecular biology and biotechnology, and provides application of a corn hypertonic gated calcium ion channel gene ZmERD4 in corn plant height regulation, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the mutation mode of the mutation genotype Zmerde4 is c.1201Ggt; glu401Lys is used as a molecular marker of Ap. According to the embodiment of the invention, the application of the corn hypertonic gating calcium ion channel gene ZmERD4 in the aspect of corn plant type establishment is analyzed and studied, and the growth and development of a corn plant are hindered and the plant height is reduced due to the overexpression of Zmede4.

This invention discloses a SNP molecular marker associated with skin color traits in Ma Huang chickens and its application, belonging to the field of genetic breeding technology. This invention identifies an SNP locus at position 49,442,841 in the intron region of the SCARB2 gene on chicken chromosome 4. This locus contains a T>G mutation, with genotypes including TT, TG, and GG. This locus is significantly correlated with the yellowness of the skin on the chest, back, and legs. Individuals with the TT genotype show significantly higher yellowness values ​​on the chest, back, and legs than individuals with the TG genotype. This indicates that this SNP locus can accurately identify skin color traits in chickens and can serve as an important genetic marker for early screening of skin color traits during chicken breeding. Early selection of chickens based on the genotype at this locus can save production costs and accelerate genetic progress.

The present application relates to the technical field of sheep high multiplication mutant genotype distinguishing, and particularly relates to a rapid genotyping method of sheep FecB mutation site based on SfaTnpB nuclease and a kit thereof.The method can not only effectively identify the genotype of the 746th position (FecB mutation) of the BMPR1B gene exon of a sheep individual, but also accurately distinguish the genotype of an individual with additional mutation at the 743th position in addition to the mutation at the 746th position in the actual production and breeding process.Compared with the prior art, the present application has the advantages of simple operation, rapid detection result judgment (within 45 min) and the like, and can provide a rapid, sensitive and good specificity FecB mutation site genotyping technical means for on-site screening of target genotype individuals in the sheep breeding process.

The invention relates to the technical field of fish genetic breeding, in particular to a gene FTR100 related to a crucian carp interferon antiviral immune response and application of the gene FTR100. Gene related to the antiviral immune response of the crucian carp interferon is FTR100, the invention further discloses three specific knockout targets of the FTR100 gene, the crucian carp FTR100 gene is specifically knocked out by utilizing a CRISPR / Cas9 gene editing technology, and a mutant genotype individual with three alleles terminated in advance, namely the crucian carp with high resistance to the crucian carp herpesvirus, is obtained. According to the invention, the function of the FTR100 in the prussian carp is verified for the first time, a new germplasm with improved capability of resisting the herpesvirus of the prussian carp is rapidly obtained by knocking out a single gene of the FTR100, and the method has important industrial value.

This invention relates to the field of soybean molecular breeding technology, and in particular to a molecular marker associated with the distichous (diragian leaf arrangement) plant architecture trait of soybean and its application. This invention identified a mutant from a soybean mutant library. From a top-down perspective, this mutant exhibits one leaf per node, with trifoliate leaves arranged in a straight line ("I"), meaning all trifoliate leaves are arranged in a planar pattern. This reduces the space occupied by a single plant and decreases plant spacing, which is beneficial for increasing density and yield. This mutant has a non-synonymous mutation (C to T) at base 121 of the Glyma.03g232100 gene, leading to the development of the CAPS molecular marker. The CAPS molecular marker of this invention can identify wild-type and mutant genotypes, contributing to the improvement of soybean plant architecture and density-tolerant breeding. Utilizing the SNP loci identified in this invention and the developed CAPS molecular marker can help improve soybean plant architecture and density-tolerant breeding, demonstrating significant breeding application potential.

The present invention discloses a method and application for rapidly detecting single nucleotide polymorphisms at the Chr1:g.61542435 site in sheep using KASP. Using sheep genomic DNA as a template and the published specific KASP primer sequence, the KASP detection method is used to rapidly and accurately type the single nucleotide polymorphisms at the Chr1:g.61542435 site in sheep. The different genotypes of the SNP site are significantly correlated with the milk protein rate trait of sheep milk, and play an important role in the molecular marker-assisted selection of milk protein rate in sheep milk. The present invention can assist in establishing a sheep genetic resource group with a high milk protein rate by rapidly and accurately detecting the mutant genotype of the Chr1:g.61542435 site related to the milk protein trait of sheep milk, thereby improving the quality of sheep milk products.

The invention discloses a KASP molecular marker remarkably related to the amylose content of wheat and application of the KASP molecular marker, and belongs to the technical field of wheat breeding. A KASP molecular marker system closely linked with SBEIIa and / or SBEIIb is developed on the basis of information of one mutation site of a wheat starch branching enzyme gene SBEIIa on a 2B chromosome and information of three mutation sites of SBEIIb on 2A, 2B and 2D chromosomes, and the KASP molecular marker system is simple and convenient in detection process, low in cost, high in throughput, high in sensitivity and high in sensitivity. According to the method, the high-amylose wheat material which contains the mutant genotype and can be stably inherited can be accurately and efficiently screened out, the breeding process of wheat variety quality improvement can be accelerated, and the method has important significance on creation of the high-amylose wheat material or molecular assisted breeding of the high-amylose wheat.

The invention relates to a cervical cancer risk assessment detection system and a kit, comprising an SNP (Single Nucleotide Polymorphism) site composite amplification detection system and a kit containing the SNP site composite amplification detection system. The SNP locus composite amplification detection system comprises a primer combination for typing detection of 10 SNP loci, and the SNP loci are respectively related to a folic acid metabolic pathway, a DNA damage repair function, detoxification metabolism, immunoregulation and a cancer suppression pathway. The kit is designed on the basis of the ARMS-PCR technology, wild type and mutant genotypes can be accurately recognized through specific primers aiming at specific SNP sites, and the kit has the advantages of being high in specificity and sensitivity; capillary electrophoresis can realize synchronous and efficient separation of multiple fragments by virtue of high resolution (fragments with length difference of 1-2bp can be distinguished), and has the characteristics of high flux and rapidness.

The application discloses a molecular marker related to a breast muscle weight trait of Zhongshan Muscovy duck and application thereof, and belongs to the technical field of molecular marker assisted poultry breeding. The molecular marker is a SNP site located at the 7467101th position of a duck genome 5th chromosome, and a G / A mutation exists, and the genotype is GG, GA or AA. By detecting the genotype of the site, the breast muscle weight trait can be determined: the breast muscle weight of an individual with the GG genotype is significantly higher than that of individuals with the GA and AA genotypes. The application can realize early and accurate selection and breeding of the breast muscle weight of the Zhongshan Muscovy duck at the living stage, avoid slaughter determination, reduce costs, improve genetic gain, and provide a reliable tool for improving the meat performance of the Zhongshan Muscovy duck.

This invention belongs to the field of animal molecular breeding technology, specifically disclosing a SNP molecular marker associated with the trait of black peritoneum in chickens and its application. Through genome-wide association analysis, this invention obtained an SNP molecular marker that is highly significantly associated with the black peritoneum trait in yellow-feathered broilers. This marker, using the chicken GRCg7b genome as a reference, is located at position 120222 on chromosome 3, and is an A / T mutation with genotypes including AA, AT, and TT. Association analysis showed that this locus was highly significantly associated with the black peritoneum phenotype observed after slaughter at 11 weeks of age. The incidence of black peritoneum in individuals with the AA genotype was significantly lower than that of individuals with the AT and TT genotypes. A is the dominant allele reducing the occurrence of black peritoneum, and T is the risk allele for black peritoneum. This invention also provides specific primers for detecting this molecular marker and a marker-assisted selection method, enabling early, non-destructive, and precise selection for the black peritoneum trait, reducing slaughter defect rates, and improving the carcass quality and economic benefits of yellow-feathered broilers.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a group of Indel molecular markers related to soybean phytophthora resistance traits and application. The molecular marker provided by the invention is developed aiming at a GmLLG1 gene and a GmLLG2 gene in a soybean Gmlg1Gmlg2 double mutant. The molecular marker group provided by the invention can accurately identify the double-mutation genotype of soybean Gmlg1Gmlg2, and soybean materials containing the double-mutation genotype can be quickly and efficiently screened by utilizing the group of molecular markers, so that early directional selection and molecular marker-assisted breeding of soybean phytophthora resistance traits are realized; the breeding efficiency of a new soybean variety resisting phytophthora sojae is remarkably improved, and a key technical support is provided for green prevention and control of the phytophthora sojae.

This application provides a detection primer, method, and application for the Y4667D / C mutation of the nicotine receptor in the rice stem borer, belonging to the field of agricultural pest resistance detection technology. The detection primer set is designed for the Y4667D / C mutation site, a target receptor for the rice stem borer's high resistance to diamide insecticides. It includes a first primer set for simultaneous detection of Y4667D and Y4667C, a second primer set for genotypic specific detection of Y4667D, and a third primer set for genotypic specific detection of Y4667C; each primer set contains 6-8 primers (outer, inner, and loop primers). The detection method primarily relies on visual interpretation of genotypes through color changes in the reaction solution, eliminating the need for a thermal cycler. It offers advantages such as multiple tests per tube, flexible splitting, and high sensitivity. This invention is suitable for rapid field detection of mutant genotypes and frequencies in the rice stem borer, providing technical support for diamide insecticide resistance monitoring and scientific management.

The invention relates to the technical field of fish genetic breeding, in particular to a method for improving carassius auratus gibelio herpesvirus resistance based on herc4 gene editing. The invention discloses three specific knockout targets of a serc4-B gene, and utilizes a CRISPR / Cas9 gene editing technology to specifically knock out the serc4-B gene of carassius auratus gibelio to obtain a mutant genotype individual with at least two alleles of serc4-B terminated in advance, namely the carassius auratus gibelio with high resistance to carassius auratus gibelio herpesvirus. According to the invention, the function of the herc4-B in the prussian carp is verified for the first time, a new germplasm with improved capability of resisting the herpesvirus of the prussian carp is rapidly obtained by knocking out the single gene of the herc4-B, and the herpesvirus of the prussian carp has important industrial value.