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Hbv B genotype 1799G>C mutation as molecular marker application and kit

A kit and genotype technology, applied in the field of prevention and treatment of hepatitis B virus

Inactive Publication Date: 2015-08-05
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although the relationship between HBV genome mutation and drug therapeutic drug resistance, the relationship between mutation and HBeAg expression, and the relationship between genotype and disease prognosis have been reported, the 1799 base G>C mutation in the BCP region (basic core promoter) of HBV genome is associated with The relationship between HBV infection, genotype, and cirrhosis / hepatocellular carcinoma has not been reported

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  • Hbv B genotype 1799G>C mutation as molecular marker application and kit

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Embodiment Construction

[0029] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0030] For HBV virus, the whole genome sequence of HBV genotype B, genotype B2, genotype C, genotype C1, and genotype C2 of HBV infected by Chinese population was searched through the database of the National Center for Health Information (NCBI).

[0031] Perform Clustal comparison of all genotypes and gene subtypes to find the inter-type conserved regions of PreS2, S gene region and BCP region. The comparison results are as follows figure 1 Shown, where the arrow marks the 1799 site.

[0032] 1. Analyze and design the amplification region and primers

[0033] On the basis of the above analysis, use Primer5 to design amplification primers as shown in Table 1, and the corresponding amplification procedures are shown in Table 2; the goal is to stably amplify the PreS2, S subtype detection sequ...

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Abstract

The invention discloses an application of HBV (hepatitis B virus) B genetype 1799G>C mutation as a molecular marker and a kit, based on extremely high correlation of the mutation at the site 1799 of the HBV B genetype with liver cirrhosis / liver cancer; the polymorphism of the 1799th site G / C as the molecular marker can be applied to preparing a reagent or a kit for prompting the disease progress and tendency of an HBV B genetype infected person, and also serves as a target spot for preparing a reagent or a medicament for reminding the HBV B genetype infected person of the possibility of the progress of evolving into liver cirrhosis / liver cancer; as a result, the progress of the course of disease of being infected by genetype B hepatitis B virus can be predicted at the early stage of infection; furthermore, assistance can be provided for treating the patient at the initial stage of infection, the progress of the liver diseases is prevented from accelerating to evolving into heavy liver diseases and even the liver cancer; moreover, the monitoring of a prognosis target can be carried out smoothly.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of hepatitis B virus, and relates to the application of HBV B genotype 1799G>C mutation as a molecular marker and a kit. Background technique [0002] A large number of literatures have reported that hepatitis B virus (HBV) is the most important factor in the occurrence of liver cirrhosis / liver cancer in my country, and more than 90% of liver cirrhosis / liver cancer patients have been found to be infected with hepatitis B virus. my country is the country with the highest incidence of hepatitis B, so early diagnosis of hepatitis B virus infection is particularly important for controlling the development of liver disease. The mechanism of liver cirrhosis / liver cancer caused by hepatitis B virus infection is not yet clear, but the phenomenon of chronic liver disease and even liver cirrhosis / liver cancer caused by the integration of HBV into the genome of human liver cells has been ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 颜真包晗王伟华
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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