Muscovy duck egg laying performance associated gene SMAD3 molecular marker screening method

Abstract

A muscovy duck egg laying trait associated gene SMAD3 molecular marker screening method comprises the following steps: (1) carrying out DNA extraction: extracting female muscovy duck genome DNA in anegg laying period by adopting a phenol-chlorine method, and then determining an OD value and concentration of the purity of the genome DNA; (2) carrying out primer design: carrying out SMAD3 gene amplification primer design by applying NCBI online software and Primer 5.0, and sending a primer sequence to a related biological company for synthesis; (3) carrying out PCR amplification: adopting a 15[mu]L reaction system, setting PCR amplification conditions, carrying out PCR amplification, and carrying out electrophoresis detection on a product by using 1% agarose gel; (4) sequencing amplification product fragments and screening SNP sites; and (5) directly sequencing and typing the SNP mutation sites, carrying out correlation analysis on the SNP mutation sites and the egg laying performance,and determining the SNP closely correlated with the egg laying performance. When the molecular marker is applied to the muscovy duck seed selection and breeding process, the mutant genotype of the gene SMAD3 associated with the muscovy duck egg laying performance detected by the method is used as the molecular marker.

Description

technical field [0001] The invention belongs to a poultry molecular marker selective breeding method, in particular to a screening method and application of a gene SMAD3 molecular marker associated with egg production traits of Muscovy ducks. Background technique [0002] Muscovy duck is one of the excellent meat duck species in China, because of its large size, easy feeding, high lean meat rate, special wild bird flavor, good fat liver production performance, rough feeding resistance, strong disease resistance, and feed conversion It has high efficiency and other advantages, and is favored by the majority of farmers and consumers. It has a broad market breeding prospect. However, compared with domestic ducks, the female Muscovy ducks take a long time to grow (24~26 weeks), which leads to increased feeding costs. The egg production is less (80-120 pieces), and the female Muscovy duck has a strong nesting ability, and the strong nesting ability will cause her ovaries to degen...

AI Technical Summary

Problems solved by technology

In recent years, research reports on the application of SNP molecular markers in poultry selection and breeding are frequent, but there are few reports on the mining and application of molecular markers for egg-laying traits of Muscovy ducks, and the mining of molecular markers of SMAD3 gene and their application in Muscovy ducks Egg production traits are still new territory

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