Screening method of molecular marker of circadian rhythm behavior related gene Cry1 of egg ducks

A circadian rhythm, molecular marker technology, applied in biochemical equipment and methods, DNA/RNA fragments, DNA preparation, etc., to achieve the effect of eliminating abnormal behavior

Inactive Publication Date: 2017-08-22
GUIZHOU UNIV
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  • Claims
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Problems solved by technology

In recent years, there have been a large number of research reports on molecular markers and their application in breeding, but there are few reports on the mining and a...

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  • Screening method of molecular marker of circadian rhythm behavior related gene Cry1 of egg ducks

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Embodiment 1

[0019] The molecular marker and application of the gene Cry1 associated with the circadian rhythm behavior of laying ducks comprises the following steps:

[0020] 1. DNA extraction

[0021] Carry out DNA extraction with blood / cell / tissue genome extraction box to the laying duck blood sample (50 parts) that needs to detect, after DNA extraction, detect with 2% agarose gel electrophoresis, and utilize ultraviolet spectrophotometer to measure its OD value and sample Concentration, stored in -20 ℃ refrigerator. The DNA pool was constructed to finally obtain the mixed DNA pool of laying ducks.

[0022] 2. Primer Design

[0023] According to the duck Cry1 gene sequence (serial number is NW_004676465.1) published in the GeneBank database, use the software Oligo6.0 and Primer5.0 to design and screen primers, and send them to Biotechnology Co., Ltd. for synthesis. The primer sequence information is shown in Table 1:

[0024] Cry1 Gene PCR Amplification Primer Sequence

[0025]

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Abstract

The invention discloses a screening method of a molecular marker of a circadian rhythm behavior related gene Cry1 of egg ducks. The screening method comprises the following steps: (1) extraction of DNA: extracting DNA by using a blood/cell/tissue genome DNA kit, and then assaying the OD value and concentration; (2) designing of a primer: designing the primer by using Primer Primer 5.0, and delivering the primer to a biology company for synthesis; (3) PCR amplification: conducting PCR amplification by using a 20mu L reaction system and setting the PCR amplification conditions, and taking and detecting the product by agarose gel electrophoresis; (4) fragment purification of the amplification product, vector linking, conversion, positive clone screening and sequencing; and (5) SNP mutant site screening and correlation analysis for determining the circadian rhythm behavior related SNP of egg ducks. In the molecular breeding process, the mutant gene type of the circadian rhythm behavior related gene Cry1 detected by the method is regarded as the molecular marker.

Description

technical field [0001] The invention belongs to a poultry molecular marker selection method, in particular to a molecular marker selection method and application of the circadian rhythm behavior-associated gene Cry1 of laying ducks. Background technique [0002] Behavior, as a complex quantitative trait, is regulated by multiple genes and affected by various environmental factors. Among these various behaviors, the circadian rhythm behavior of poultry is often ignored by people, but it affects the welfare of poultry in large-scale intensive farming in Europe, America and China. It not only affects the egg production of poultry, but also affects the growth of poultry. Studies have shown that appropriate light intensity and time can make laying birds feel comfortable and can better develop the productivity of poultry, which is related to the cryptochrome gene Cry 1 to a certain extent. [0003] In the past, light was used as an environmental factor to study how it affects the...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6806C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨胜林陆曼王单娜柳诚刚王旭平周美迪谭斌杨汝才
Owner GUIZHOU UNIV
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