Identification method and special marker for red feather mutagenic genotype of white leghorn

A genotype and heterozygous genotype technology is applied in the field of identification of genotypes caused by red feathers in White Leghorn chickens and special markers. It does not show problems such as red feather phenotype, and achieves the effect of saving breeding cost and time, increasing variety benefits and improving use value.

Active Publication Date: 2018-05-29
CHINA AGRI UNIV +1
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] White Leghorn chickens carry the gene mutation sites related to red feathers, but do not show the red feather phenotype. It can only be speculated whether the hen carries the red feather mutation by crossing with Luodaohong and other colored feather roosters, while the rooster Can only speculate based on the presumed results of its full sibling hens
This traditional method cannot accurately distinguish between homozygous and heterozygous individuals for the red feather causative mutation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Discovery of causative mutations in Bailaihang chicken red feathers and preparation of amplification primers and establishment of methods

[0054] 1. Discovery of the causative mutation in the red feather of Bailaihang chicken

[0055] 1. Extract the genomic DNA of the chicken to be tested

[0056] Using 2000 Beijing Huadu Yukou Poultry Industry Co., Ltd. Bailaihang strain hens to cross with Luodao red roosters, the feather color phenotypes of the hybrid offspring were counted, and the number of offspring hens was more than 5 and 100% were red feathers. 9 aircraft carrier chickens were used as the red feather group, and 9 white aircraft carrier chickens with more than 5 offspring hens and 100% white feathers were selected as the white feather group; these two groups were used as chickens to be tested.

[0057] Blood was collected from the wing veins of the chickens to be tested, treated with anticoagulants, lysed and digested with protease, and then the geno...

Embodiment 2

[0112] Example 2. Identifying the genotype of the causative mutation in the red feather of the White Legion chicken to be tested by using the SNP loci of the red feather of the White Legion chicken

[0113] 1. Use the SNP loci of Bailaihang chicken red feather to identify the genotype of the causative mutation in Bailaihang chicken red feather

[0114] 1. Genomic DNA extraction

[0115] Genomic DNA of 1573 Bailai aircraft carrier chickens to be tested was extracted.

[0116] 2. PCR amplification

[0117] Using the genomic DNA of 1,573 white-born aircraft carrier chickens to be tested as templates, PCR amplification was carried out by the method described above in Example 1 to obtain the PCR amplification products of each chicken to be tested.

[0118] 3. Sequencing and analysis

[0119] The PCR amplification products of the above-mentioned chickens to be tested were subjected to DNA Sanger sequencing analysis, and the sequencing result of the PCR was sequence 1. According t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a red feather mutant of white leghorn as well as a marker and a detection method of the red feather mutant. The invention provides application of a substance for detecting thegenotype of red feather SNP (Single Nucleotide Polymorphism) luci in a genome of the white leghorn in identification of color characters of feather of filial generations of to-be-detected white leghorns and Rhode Island Red cocks; the red feather SNP loci are the 18th,, 288th and 303rd loca of a positive-sense strand of No.11 chromosome of a chicken genome. The experiment of the invention proves that one SNP locus is found and can realize identification of the red feather mutagenic genotype; by using the method, the red feather mutagenic genotype of white leghorn strain chicken is successfullyidentified. The identification method is convenient and quick, can be used for quickly cultivating a novel red feather mutant homozygotic type strain and further is applied to cultivation of a novelcomplete strain of 100 percent red feather phenotype red feather pink egg-shell chickens; cultivation cost is reduced, cultivation time is shortened and variety benefits are increased.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to the identification and special markers of the genotype of the red feather of the Bailaihang chicken causing the mutation. Background technique [0002] Bailaihang chicken is a famous egg-type breed in the world. It is widely used in the cultivation of new matching lines and has made great contributions to poultry breeding. The whole body feathers of Bailaihang laying hens are white, belonging to dominant white feathers. Previous studies have shown that the 9 bp insertion mutation in exon 10 of the PMEL17 gene in Bailaihang chickens is the main cause of the dominant white feather trait, and the corresponding allele is I (Kerje S, Sharma P U, Kim H, et al. al. The Dominant white, Dun and Smoky color variants in chicken are associated with insertion / deletion polymorphisms in the PMEL17 gene. [J]. Genetics, 2004, 168(3):1507-1518.). The dominant allele I will inhibit the s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
Inventor 杨宁李光奇吴桂琴孙从佼孙皓石凤英
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap