Identification method and special marker for red feather mutagenic genotype of white leghorn

Abstract

The invention discloses a red feather mutant of white leghorn as well as a marker and a detection method of the red feather mutant. The invention provides application of a substance for detecting thegenotype of red feather SNP (Single Nucleotide Polymorphism) luci in a genome of the white leghorn in identification of color characters of feather of filial generations of to-be-detected white leghorns and Rhode Island Red cocks; the red feather SNP loci are the 18th,, 288th and 303rd loca of a positive-sense strand of No.11 chromosome of a chicken genome. The experiment of the invention proves that one SNP locus is found and can realize identification of the red feather mutagenic genotype; by using the method, the red feather mutagenic genotype of white leghorn strain chicken is successfullyidentified. The identification method is convenient and quick, can be used for quickly cultivating a novel red feather mutant homozygotic type strain and further is applied to cultivation of a novelcomplete strain of 100 percent red feather phenotype red feather pink egg-shell chickens; cultivation cost is reduced, cultivation time is shortened and variety benefits are increased.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to the identification and special markers of the genotype of the red feather of the Bailaihang chicken causing the mutation. Background technique [0002] Bailaihang chicken is a famous egg-type breed in the world. It is widely used in the cultivation of new matching lines and has made great contributions to poultry breeding. The whole body feathers of Bailaihang laying hens are white, belonging to dominant white feathers. Previous studies have shown that the 9 bp insertion mutation in exon 10 of the PMEL17 gene in Bailaihang chickens is the main cause of the dominant white feather trait, and the corresponding allele is I (Kerje S, Sharma P U, Kim H, et al. al. The Dominant white, Dun and Smoky color variants in chicken are associated with insertion / deletion polymorphisms in the PMEL17 gene. [J]. Genetics, 2004, 168(3):1507-1518.). The dominant allele I will inhibit the s...

AI Technical Summary

Problems solved by technology

[0005] White Leghorn chickens carry the gene mutation sites related to red feathers, but do not show the red feather phenotype. It can only be speculated whether the hen carries the red feather mutation by crossing with Luodaohong and other colored feather roosters, while the rooster Can only speculate based on the presumed results of its full sibling hens

This traditional method cannot accurately distinguish between homozygous and heterozygous individuals for the red feather causative mutation