Kit for accurately detecting polymorphism of ALDH2 (Aldehyde Dehydrogenase 2) gene

Abstract

The invention belongs to the technical field of biological detection and discloses a kit for accurately detecting the polymorphism of an ALDH2 (Aldehyde Dehydrogenase 2) gene. The kit for accurately detecting the polymorphism of the ALDH2 gene can be used for determining mutation gene types (G>A, G>T and G>C) of rs671 polymorphism sites of the ALDH2 gene in one step. In order to realize the function, the kit applies a method combining a multi-PCR (Polymerase Chain Reaction) technology and a Taqman-MGB fluorescent probe labeling technology, and four pairs of specific primers and four specific probes for labeling different fluorescent dyestuffs are designed; the kit is characterized in that a target gene only can be combined with one pair of primers and one probe, so that the accuracy of a detection result is ensured. The kit provided by the invention has the advantages of strong specificity, high accuracy, simplicity in operation and the like. A sample to be detected can be selected from whole blood or oral cells; the kit is clinically used for guiding rational drug use of nitroglycerin and guiding healthy wine drinking to prevent alcohol intoxication, and has great significance inthe aspect of prompting major diseases.

Description

technical field [0001] The invention relates to gene polymorphism detection in the field of biomedicine, in particular to an acetaldehyde dehydrogenase 2 (ALDH2) gene polymorphism detection kit. Background technique [0002] Aldehyde dehydrogenase 2 (aldehyde dehydrogenase, ALDH2), located on chromosome 12, its main polymorphism is rs671. The ALDH2 gene contains 13 exons, and there is a single nucleotide polymorphism site at exon 12, where guanine nucleotides are mutated to adenine nucleotides, that is, G→A, alleles The mutation of ALDH2 causes the amino acid encoded by it to change from glutamic acid to lysine, thereby reducing or even inactivating the activity of ALDH2 enzyme. The dehydrogenase activities of ALDH2*1 / *2 and ALDH2*2 / *2 are only 13%-14% and 2% of ALDH2*1 / *1, respectively, and the catalytic efficiencies of nitroglycerin in people with mutations at rs671 are 8% to 15% and 6% to 7% of the population without mutations. [0003] ALDH2 can oxidize acetaldehyde t...

AI Technical Summary

Problems solved by technology

The above methods all need to extract the DNA in the sample to be tested, and some methodologies need to use gel electrophoresis to analyze the results, the operation process is complicated, and the detection cycle is long

During the detection process, only the mutation of the allele G to A can be detected, and it is easy to cause false negatives or false positives for samples with G mutations to T and C, resulting in wrong diagnostic results

Images