65 results about "ALDH2 gene" patented technology

The invention discloses a genetically engineered bacterium for expressing a human derived acetaldehyde dehydrogenase gene and application thereof, and belongs to the technical field of gene engineering and enzyme. The ALDH2 gene efficient expression component Trp1L-URA3-TPIp-ALDH2-TPIt-Trp1R is integrated into the genome of Saccharomyces cerevisiae W303-1A, the stability of gene expression is improved compared with free plasmid expression, a constitutive promoter is used, no induction agent is added during fermentation, and the product is relatively safe. When fermentation is performed for 96hours, the specific activity of producing acetaldehyde dehydrogenase reaches 0.97 U / mg. The specific activity of the producing acetaldehyde dehydrogenase reaches 6.256 U / mg in ethanol with the concentration of 250 mg / 100 mL, and the specific activity of the producing aldehyde dehydrogenase in a buffer with pH of 3 reaches 0.301 U / mg. The Saccharomyces cerevisiae is selected as a host strain, on the one hand, the activity of the enzyme is kept, on the other hand, product safety is improved, and a new approach and a theoretical basis are provided for the research and development of antialcoholicdrugs.

The invention belongs to the technical field of molecular biological gene polymorphism detection, and in particular relates to a kit and a method for genotyping detection. The kit consists of a firstprimer pair and a first molecular beacon probe for ADH1B gene rs1229984 site, a second primer pair and a second molecular beacon probe for ALDH2 gene rs671 site, a third primer pair and a third molecular beacon probe for CYP1A2 gene rs762551 site, and a fourth primer pair and a fourth molecular beacon probe for TMPRSS6 gene rs855791 site. With the application of the kit provided by the invention,four genotypes corresponding to the genes and corresponding metabolic absorption types can be rapidly and conveniently detected.

The invention belongs to the technical field of biology and specifically relates to a human ALDH2 gene polymorphism detection kit as well as a preparation method and application thereof. The kit is prepared from PCR premixed reaction liquid, a positive control product and a negative control product, wherein the PCR premixed reaction liquid is used for amplifying a G1510A site of ALDH2 gene; the PCR premixed reaction liquid is prepared from a specificity primer sequence set, a probe set and PCR reaction liquid; the specificity primer sequence set is used for amplifying all the sites; the specificity primer set is prepared from an ordinary outer primer and an ARMs primer with fluorescent label specificity. The kit disclosed by the invention is used for detecting the human ALDH2 gene polymorphism and has the advantages of high flexibility, high specificity, convenience in operation, reliable results and the like; furthermore, detection can be finished within 1 hour, and result reading issimple and objective.

The invention discloses a primer and a kit for detecting genetic typing of human aldehyde dehydrogenase 2 (ALDH2). The primer group for detecting human ALDH2 gene typing comprises an ALDH2*1 primer pair, an ALDH2*2 primer pair and an internal reference primer pair. The application of the kit helps to realize good typing for ALDH2 gene, and has reference value for hereditary dangerous factors such as drinking behavior, alcoholic cirrhosis, liver cancer, nasopharyngeal carcinoma, oral cancer and the like, and glyceryl trinitrate nitroglycerin guiding pharmacy.

The invention discloses a kit for detecting acetaldehyde dehydrogenase 2 (ALDH2) gene polymorphism by a pyro-sequencing method and a method. By the kit, the ALDH2 gene polymorphism, particularly RS671 single nucleotide polymorphism can be detected. The kit comprises primers which are shown as SEQ ID NO.2-4. By the kit, the accurate, quick and high-throughout detection of ALDH2 gene polymorphism can be realized, so that the alcohol tolerance can be effectively predicted and prevented, and the clinical individual administration of nitroglycerin can be guided.

The invention provides a gene detection reagent kit for evaluating alcohol tolerance. The gene detection reagent kit comprises six amplification primers for three genes including an internal reference gene GAPDH (glyceraldehyde phosphate dehydrogenase), a gene ADH1B (ethanol dehydrogenase 1B) and a gene ALDH2 (acetaldehyde dehydrogenase 2) and five Taq-man probe primers. One of the Taq-man probe primers is positioned at the internal reference gene GAPDH and is used for carrying out FAM fluorescence labeling; two other Taq-man probe primers are SNP (single nucleotide polymorphism) probes positioned at the gene ADH1B and are used for carrying out VIC and ROX (roxithromycin) labeling; the remaining two Taq-man probe primers are SNP probes positioned at the gene ALDH2 and are used for Cy5 and Quasar705 labeling, BHQ is used as a quenching group, and delta ct values can be computed. The gene detection reagent kit has the advantages that the alcohol tolerance of healthy persons can be evaluated by the gene detection reagent kit from the aspect of genes, and accordingly the healthy persons can know the strength and the weakness of alcohol tolerance, can drink alcohol scientifically and can carry on occasion such as party, gathering and banquet related to alcohol culture.

A primer set and probe set for predicting alcohol-degrading ability and hangover development is provided to improve rapidness and convenience of prediction by amplifying at least one target sequence selected from ALDH2(acetaldehyde dehydrogenase 2) gene, CYP2E1(cytochrome P450 2E1) gene and ADH2(alcohol dehydrogenase 2) gene. An oligonucleotide probe set capable of hybridizable with at least one target sequence selected from exon XII region of ALDH2 gene, 5'-regulating region of CYP2E1 gene and the exon III region and exon IX region of ADH2 gene is selected from (1) an oligonucleotide probe capable of hybridizable with the exon XII region of ALDH2 gene containing 10 or more consecutive nucleotide fragments containing a 11st base in the nucleotide sequence of SEQ ID NO:15, (2) an oligonucleotide probe capable of hybridizable with the 5-regulating region of CYP2E1 gene containing 10 or more consecutive nucleotide fragments containing a 12nd base in the nucleotide sequence of SEQ ID NO:17, (3) an oligonucleotide probe capable of hybridizable with the exon III region of ADH gene containing 10 or more consecutive nucleotide fragments containing a 12nd base in the nucleotide sequence of SEQ ID NO:19, (4) an oligonucleotide probe capable of hybridizable with the exon IX region of ADH2 gene containing 10 or more consecutive nucleotide fragments containing a 12nd base in the nucleotide sequence of SEQ ID NO:21, and (5) an oligonucleotide probe capable of hybridizable with the exon XII region of ADH2 gene containing 10 or more consecutive nucleotide fragments containing a 13rd base in the nucleotide sequence of SEQ ID NO:23.

The invention discloses a method, primer and detection kit for polymorphism detection of two genes of ethanol dehydrogenase ADH1B and acetaldehyde dehydrogenase ALDH2 which are relative to alcohol metabolism. Both the primer and the detection kit comprise forward and reserve primers amplifying polymorphism sites of the ethanol dehydrogenase ADH1B and the acetaldehyde dehydrogenase ALDH2 and sequencing primers. According to the method, primer and detection kit for polymorphism detection of the two genes of the ethanol dehydrogenase ADH1B and the acetaldehyde dehydrogenase ALDH2 which are relative to alcohol metabolism, a Sanger sequencing method is adopted, and the primer is used for fast detecting mutation situations of the polymorphism sites of the ethanol dehydrogenase ADH1B and acetaldehyde dehydrogenase ALDH2.