Kit and method for detecting ALDH2 polymorphism based on FRET probe melting curve

A gene polymorphism and melting curve technology, which is applied in the field of kits for detecting ALDH2 gene polymorphism based on the FRET probe melting curve method, can solve the problems of complicated operation, high synthesis cost, aerosol pollution, etc., and achieves easy operation. , high sensitivity, the effect of avoiding pollution
CN105063178AInactive Publication Date: 2015-11-18SHANGHAI GOOD SCI BIOTECH
2 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI GOOD SCI BIOTECH
Publication Date
2015-11-18
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention discloses a kit and a method for detecting ALDH2 polymorphism based on a FRET probe melting curve. The kit comprises a forward primer and a reverse primer for specifically amplifying the gene ALDH2 (rs671) locus, and a pair of fluorescently labeled probes for specifically distinguishing the gene ALDH2 (rs671) polymorphism, wherein the specific sequence of the forward primer is F: 5'-AGCAGACCCTAAATCCCTGG-3, the specific sequence of the reverse primer is R: 5'-CACCAGCAGACCCTCAAG-3', the specific sequence of the sensor probe is 5'-TGCAGGCATACACTGAAGTGAAAACTGT-FAM-3', and the specific sequence of the anchor probe is 5'-ROX-AGTGTGGGACCTGCTGGGGGCT-P-3'. According to the present invention, the advantages of good specificity, high sensitivity, low price, simple operation and avoidance of pollution caused by tune opening operation are provided, and the result can be intuitively and clearly interpreted through the TM value read by the instrument.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of in vitro nucleic acid detection, and in particular relates to a kit and a method for detecting ALDH2 gene polymorphism based on a FRET probe melting curve method. Background technique

[0002] Human acetaldehyde dehydrogenase 2 (ALDH2) is one of the most important enzymes in the pathway of ethanol metabolism, and its coding gene ALDH2 has a high degree of polymorphism. In Asian yellow populations, ALDH2 is the most frequent and most important mutant. Recent studies have shown that ALDH2 can inhibit its carrier's dependence on alcohol to a certain extent, and has a profound relationship with alcoholism, alcoholic liver disease, upper gastrointestinal cancer and gastric cancer caused by alcoholism.

[0003] The ALDH2 gene mutation also reduces the activity of acetaldehyde dehydrogenase by more than 10 times, which hinders the metabolic process of alcohol from ethanol → acetaldehyde → acetic acid → water and carbon d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More