35results about How to "The reaction procedure is simple" patented technology

The invention discloses a RT-RPA-lateral flow tomography kit for doubly detecting hepatitis e virus and hepatitis a virus. The kit comprises an upstream primer, an intermediate probe and a downstreamprimer which are applicable to hepatitis e virus ORF2 gene sequence in the RT-RAP amplification technology, and/ or an upstream primer, an intermediate probe and a downstream primer which are applicable to hepatitis a virus VP1 gene sequence, general agents for recombinase polymerase amplification technology, and a lateral flow tomography test strip, wherein the lateral flow tomography test stripcomprises a sample adding pad, a control line, a #1 detecting line and/ or a #2 detecting line; the control line, the detecting lines and the primers are combined with a probe label. With the adoptionof the kit, the hepatitis e virus ORF2 gene and/ or hepatitis a virus VP1 gene in a sample can be rapidly, sensitively and specifically detected on site in a non-lab environment.

The present invention is process of activating carbon nanotube in great specific surface area, and belongs to the field of nanometer material technology. The technological process includes the following steps: 1. mixing KOH and carbon nanotube in the mass ratio of 3-5 physically and stoving; 2. reacting in a horizontal tubular quartz furnace for 0.5-2 hr through introducing inert gas, raising the temperature in the rate of 15 deg.c/min to the reaction temperature 700-900 deg.c and regulating gas carrier flow rate to 80-100 l/hr; and 3. collecting the reaction product, acid washing the product with dilute hydrochloric acid solution, water washing, filtering and stoving. The process has facile material, low cost, no environmental pollution, obvious activating effect and other advantages, and the activated carbon nanotube possesses specific surface area of 400-1100 sq m/g and pore volume of 0.8-1.7 cu cm/g.

The invention is suitable for the field of chemical pharmacy and provides a method for preparing ceftazidime hydrochloride. The method comprises a reaction of carrying out acylation to form ester and a hydrolysis reaction. The method for preparing the ceftazidime hydrochloride, which is disclosed by the invention, greatly simplifies the reaction process an reduces use of raw materials and energy by omitting steps of filtering, extracting, drying and the like after the esterification reaction, so that production cost is greatly reduced; and moreover, the method prevents loss of ceftazidime tert-butyl ester and improves production benefits.

The invention discloses a method for rapidly synthesizing a nanometer 3A molecular sieve. The method is implemented in a way that aluminum hydroxide, sodium hydroxide, potassium hydroxide, potassium silicate and amorphous silicon dioxide are taken as raw materials for synthesizing the nanometer 3A molecular sieve. The method for synthesizing the nanometer 3A molecular sieve comprises the following steps of: dissolving an aluminum hydroxide solid into a mixed solution of sodium hydroxide, potassium hydroxide and potassium silicate; heating and crystalizing while stirring, wherein the alkalinity of the solution is gradually increased along with crystallization; adding aluminum hydroxide and amorphous silicon dioxide for reacting with an alkali; continually crystalizing a product; and filtering and drying a crystallization product to obtain a nanometer 3A molecular sieve product. The method has the characteristics that: (1) the reaction program and process flow are simple, and the synthesis time is short; (2) the raw material utilization ratio is high, and the prepared 3A molecular sieve has low pH value and is not required to be washed; and (3) the prepared nanometer 3A molecular sieve has the advantages of high product purity, large specific surface area, high selective adsorption performance and the like, and has low price.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

In reacting pentose-1-phosphoric acid with a nucleic acid base or a nucleic acid base analogue in an aqueous reaction medium in the presence of a metal cation to produce a nucleoside compound, the timing or method of addition of at least one of these components to the aqueous reaction medium is varied; thereby, a nucleoside compound can be produced at a high yield efficiently without inviting the high viscosity or solidification of the reaction mixture, even when the above components are used in such amounts that the reaction mixture becomes highly viscous or is solidified when the components are used without the above variation of the addition timing or method. Thus, there can be provided a process for producing a nucleoside compound, which comprises a step of reacting pentose-1-phosphoric acid with a nucleic acid base or a nucleic acid base analogue in the presence of nucleoside phosphorylase activity, which gives a nucleoside compound at an improved conversion, and which has wide applicability.

The present invention provides a solid phase synthesis method of ceftazidime hydrochloride, and the solid phase synthesis method is characterized in that: a raw material A is bridged on a solid support, and the solid support is removed after amidation, nitrosation, condensation cyclization reaction and etherification to obtain the ceftazidime hydrochlorid; according to the solid phase synthesis method for preparation of the ceftazidime hydrochloride, a postprocessing process after the reaction can be omitted, reaction procedures are greatly simplified, product loss in the postprocessing process can be reduced, the total yield is increased to more than 60%, the purity is increased to more than 99.5%, and production efficiency is improved.

The invention provides a DNA library construction kit based on an illumina sequencing platform, a library construction method and application. The kit comprises a P7 linker, a P5 linker, an enzyme composition or a buffer solution, wherein the enzyme composition comprises any one or a combination of at least two of a terminal repair enzyme, a PCR amplification enzyme or a T4DNA ligase; and the buffer solution comprises a tail end repairing buffer solution and/or a connecting buffer solution. According to the kit disclosed by the invention, a reaction solution is prepared by adopting commercially available reagents, so that the library construction cost is reduced, the reaction procedure is simplified, a terminal repair reaction system and an A-adding reaction system are combined in one system, the reaction time is shortened, and the enzyme connection efficiency is improved.

The invention discloses a primer pair composition for identification or assisted identification of H6N1 subtype of avian influenza virus and application of the primer pair composition. The primer pair composition for identification or assisted identification of H6 and/or N1 subtype of avian influenza virus is composed of two primer pairs which are independently packaged, namely a polymerase chain reactor (PCR) primer pair named as H6 and a PCR primer pair named as N1; the H6 is composed of two single-stranded DNA as shown in SEQ ID NO. 1 and SEQ ID NO. 2; and the N1 is composed of two single-stranded DNA as shown in SEQ ID NO. 3 and SEQ ID NO. 4.

The invention discloses a primer, kit and method for detecting bordetella pertussis (BP), and belongs to the field of molecular biology. The primer is formed by a forward primer with a nucleotide sequence as shown in SEQ ID NO: 1 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO: 2; the forward primer is labeled with biotin; and the reverse primer is labeled with FITC (Fluorescein Isothiocyanate) or Dig. According to the invention, a recombinase-polymerase nucleic acid amplification technology and a solid-phase developing technology are adopted; only isothermal amplification is performed after DNA (Deoxyribonucleic acid) extraction is performed on a clinical sample; a PCR (Polymerase Chain Reaction) instrument does not need to perform a heat cycling reaction; and a detection result can be directly read by naked eyes from a solid-phase developing test strip. The primer disclosed by the invention is strong in specificity for detecting the bordetella pertussis BP, andhigh in amplification efficiency. The kit and the detection method disclosed by the invention are simple in reaction program, capable of effectively and rapidly detecting the bordetella pertussis BP,and suitable for convenient, rapid and accurate detection of pertussis on site.

The invention provides a solid-phase synthesizing method of ceftriaxone sodium. The solid-phase synthesizing method is characterized by including: bridging raw material A to a solid-phase carrier, performing substitution and acylation reactions, and separating from the solid-phase carrier to obtain the ceftriaxone sodium. By using the solid-phase synthesizing method of the ceftriaxone sodium, the post-processing process after the reactions can be omitted, the reaction procedures are simplified greatly, product loss during the post-processing is lowered, the total yield of the reactions is increased to 90% and above, the purity of the ceftriaxone sodium is increased to 99.5% and above, and production benefits are increased.

The invention discloses a primer, a reagent kit and a detection method of detecting a short spine syndrome of dairy cattle and belongs to the field of molecular biology. The primer comprises a forwardprimer with a nucleotide sequence shown as SEQ ID NO (sequence identifier number): 1, and a reverse primer with a nucleotide sequence shown as SEQ ID NO: 2, wherein the forward primer is labeled withbiotin; and the reverse primer is labeled with digoxin. A recombinase-polymerase nucleic acid amplification technology and a lateral flow chromatographic technology are adopted; isothermal amplification is conducted on a clinical sample after DNA (deoxyribonucleic acid) extraction; a PCR (polymerase chain reaction) instrument is not required for a thermal circulation reaction; and a detection result can be directly read from a lateral flow chromatographic test strip by naked eyes. The primer has strong specificity and high amplification efficiency for detecting the short spine syndrome of thedairy cattle. The reagent kit and the detection method are simple in reaction procedure, can effectively and quickly detect the short spine syndrome of the dairy cattle and are applicable to simple,convenient, quick and accurate diagnosis of the short spine syndrome in a cattle farm.

The invention discloses a primer pair combination and an application thereof for identifying or helping identifying an avian influenza virus and an H6N1 subtype thereof. The primer pair combination and the application thereof for identifying or helping identifying the avian influenza virus and the H6N1 subtype thereof, which are provided by the invention, refer to a combination 1, a combination 2 or a combination 3. The combination 1 consists of a PCR (Polymerase Chain Reaction) primer pair of which the name is H6, a PCR primer pair of which the name is N1 and a PCR primer pair of which the name is M; the H6 consists of two single-chain DNAs shown as SEQ ID No.1 and SEQ ID No.2 in a sequence list; N1 consists of two single-chain DNAs shown as SEQ ID No.3 and SEQ ID No.4 in the sequence list; M consists of two single-chain DNAs shown as SEQ ID No.5 and SEQ ID No.6 in the sequence list; the combination 2 consists of the H6 and the M; the combination 3 consists of the N1 and the M.

The invention is applicable to the field of chemical pharmaceutical preparation, and provides a method for preparing cefotaxime through a solid-phase synthetic method. The method comprises the steps of bridging connection of raw material 7-ACA and a solid phase, solid phase synthesis and a separation reaction of a product and the solid phase. The preparation method for the cefotaxime provided by the invention avoids the steps such as post-treatment, purification and drying in the reaction process, greatly simplifies the reaction operation, reduces impurities in the reaction process, and improves the production benefits and the product quality.