Process for producing nucleoside compound

Abstract

In reacting pentose-1-phosphoric acid with a nucleic acid base or a nucleic acid base analogue in an aqueous reaction medium in the presence of a metal cation to produce a nucleoside compound, the timing or method of addition of at least one of these components to the aqueous reaction medium is varied; thereby, a nucleoside compound can be produced at a high yield efficiently without inviting the high viscosity or solidification of the reaction mixture, even when the above components are used in such amounts that the reaction mixture becomes highly viscous or is solidified when the components are used without the above variation of the addition timing or method. Thus, there can be provided a process for producing a nucleoside compound, which comprises a step of reacting pentose-1-phosphoric acid with a nucleic acid base or a nucleic acid base analogue in the presence of nucleoside phosphorylase activity, which gives a nucleoside compound at an improved conversion, and which has wide applicability.

Description

technical field [0001] The present invention relates to methods of producing nucleoside compounds using nucleoside phosphorylases. Various nucleosides and their analogous compounds are used as raw materials for antiviral drugs, antitumor drugs, antisense drugs, etc., or as components in these drugs. Background technique [0002] Nucleoside phosphorylase is a generic term for enzymes which, in the presence of phosphoric acid, cause the phosphorylation of the N-glycosidic bond of nucleosides and, in the case of ribonucleosides, catalyze the reaction represented by the following reaction reaction: [0003] <chemistry num="001"> <chem file="01819483_cml001.xml" / > < / chemistry> [0004] Nucleoside phosphorylases are mainly divided into purine nucleoside phosphorylases and pyrimidine nucleoside phosphorylases, which widely exist in the biological world and exist in tissues of mammals, birds, fish, etc., as well as yeast and in bacteria. The above enzymatic...

AI Technical Summary

Problems solved by technology

[0006] However, since the reaction of producing a nucleoside compound from pentose-1-phosphate or its salt and a nucleic acid base by using the above-mentioned reverse reaction in the presence of an enzyme is a reaction in an equilibrium state, the disadvantage of this reaction is is a low conversion rate