Human ALDH2 gene polymorphism detection kit as well as preparation method and application thereof

A detection kit and gene technology, which can be used in biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, etc., can solve the problems of long experiment time, low sensitivity of PCR-sequencing method, and unsuitable detection of mutation frequency. , to prevent false negative and false positive results, improve specificity and accuracy, and interpret the results easily and objectively
CN109055367AActive Publication Date: 2018-12-21WUHAN HEALTHCHART BIOLOGICAL TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN HEALTHCHART BIOLOGICAL TECH
Publication Date
2018-12-21

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Abstract

The invention belongs to the technical field of biology and specifically relates to a human ALDH2 gene polymorphism detection kit as well as a preparation method and application thereof. The kit is prepared from PCR premixed reaction liquid, a positive control product and a negative control product, wherein the PCR premixed reaction liquid is used for amplifying a G1510A site of ALDH2 gene; the PCR premixed reaction liquid is prepared from a specificity primer sequence set, a probe set and PCR reaction liquid; the specificity primer sequence set is used for amplifying all the sites; the specificity primer set is prepared from an ordinary outer primer and an ARMs primer with fluorescent label specificity. The kit disclosed by the invention is used for detecting the human ALDH2 gene polymorphism and has the advantages of high flexibility, high specificity, convenience in operation, reliable results and the like; furthermore, detection can be finished within 1 hour, and result reading issimple and objective.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a human ALDH2 gene polymorphism detection kit and its preparation method and application. Background technique

[0002] The human aldehyde dehydrogenase 2 (aldehyde dehydrogenase, ALDH2) gene is located on chromosome 12, contains 13 exons, and encodes two isomerases of acetaldehyde dehydrogenase and nitrate esterase. ALDH2 is an NAD(P)+-dependent enzyme, which is widely involved in the oxidation reaction of aldehydes produced during the metabolism of ethanol, amino acids, biogenic amines, vitamins, cholesterol and lipids in the body, and becomes the corresponding carboxylic acid, which is beneficial for alleviating Aldehydes play an important role in the toxicity of the body. Many mutants such as His47Arg, Glu479Lys, and Glu504Lys have been found so far, among which the main mutant is Glu504Lys (rs671), which is the single-base mutation G1510A located in exon 12. The no...

Claims

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