The invention discloses a polymorphism detection system and a kit for a gene relevant to personalized medication for a cardiovascular disease. The system relates to a quantitive fluorescent PCR (Polymerase Chain Reaction) amplification (Quantitive Fluorescent PCR, QF-PCR) technique and a capillary electrophoresis detection technique. The system has the characteristics that 1, through the multiplePCR, the amplification of 27 polymorphic sites of 17 genes is realized in a one-tube manner; 2, the reference can be provided for the use of common medicines of an antihypertensive drug, an antiplatelet drug, oral anticoagulant drug, a blood lipid regulation drug and the like by a detection result; 3, the system can be used for realizing the direct amplification of blood and blood card, and is used for avoiding the step of extracting DNA (Deoxyribonucleic Acid); 4,the system can be used for integrating a UDG-dUTP (Uracil DNA Glycosylase-Deoxyuridine Triphosphate) pollution prevention measure,and can be used for effectively preventing a product from being polluted. The polymorphism detection system and the kit have the advantages of being complete in detection site coverage, high in specificity, high in sensitivity, high in reliability, simple and convenient to operate, high in practicability and low in cost, and has large-batch detection ability.