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Klebsiella pneumoniae nucleic acid detection kit (PCR-fluorescent probe method)

A technology of Klebsiella pneumoniae and oligonucleotide probes, applied in the fields of fluorescence/phosphorescence, microbial measurement/testing, biochemical equipment and methods, etc., can solve problems such as atypical clinical manifestations and difficult diagnosis, and achieve Avoid false positive results, fast detection speed, and the effect of preventing contamination

Inactive Publication Date: 2013-06-19
DAAN GENE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in patients with serious primary diseases, the clinical manifestations are often atypical, and the diagnosis is difficult

Method used

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  • Klebsiella pneumoniae nucleic acid detection kit (PCR-fluorescent probe method)
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  • Klebsiella pneumoniae nucleic acid detection kit (PCR-fluorescent probe method)

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: KP kit detection method of Klebsiella pneumoniae

[0038] (1) Specimen collection, transportation and preservation

[0039] Specimens were collected by clinicians according to the actual situation. Testable specimens include throat swabs, nasopharyngeal secretions, or sputum. The collection methods are as follows: ① Pharyngeal swab: take the pharyngeal secretions with a cotton swab, put the cotton swab into a sterile glass tube, and seal it for inspection; ② nasopharyngeal secretions: absorb the nasopharyngeal secretions under negative pressure, seal them Submit for inspection; ③Human sputum specimen: Aseptically collect and instruct the patient to cough up sputum (not containing saliva) from the deep part of the respiratory tract, put it in a 40ml sterile plastic container, cover the container, and seal it for inspection. Specimens can be used for testing immediately, or can be stored at -70°C for testing, and the storage period is 6 months. Specimens ...

Embodiment 2

[0063] Example 2: Preparation and use of quality control products in the Klebsiella pneumoniae nucleic acid detection kit

[0064] The quality control products in the Klebsiella pneumoniae nucleic acid detection kit include positive quality control products and negative quality control products, which are used for quality control in clinical trials. KP positive quality control products are directly added to the machine after centrifugation, and negative quality control products are operated The method is the same as that of the sample to be tested.

[0065] Quality control standard: It is required to meet the following conditions in one experiment at the same time - the positive quality control product is positive (see attached Figure 5 ), the negative quality control was negative (see attached Image 6 ); otherwise, the result is invalid and needs to be tested again.

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Abstract

The invention relates to a real-time fluorescent PCR kit, and especially relates to a kit used for detecting klebsiella pneumonia (KP) by using a real-time fluorescent PCR technology. With the kit, KP in samples can be detected. The kit can be widely applied in auxiliary diagnosis of KP infection.

Description

technical field [0001] The invention relates to a real-time fluorescent PCR kit, in particular to a kit for detecting Klebsiella pneumoniae (Klebsiella pneumoniae, KP) by using real-time fluorescent PCR technology. The kit can be widely used in auxiliary diagnosis of Klebsiella pneumoniae infection by detecting Klebsiella pneumoniae in samples. Background technique [0002] Klebsiella pneumoniae is the most common Gram-negative bacillus in clinic, and it is an important pathogen that causes hospital-acquired urinary system infection, hospital-acquired pneumonia and abdominal infection. At the same time, Klebsiella pneumoniae is also a potential pathogen of community-acquired infections such as community-acquired pneumonia. Klebsiella pneumoniae (KP) is widely distributed in nature and in the gastrointestinal tract of humans and animals. It is a common bacterium in clinical specimens. When the body's resistance is reduced, it enters the lungs through the respiratory tract an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04G01N21/64
Inventor 李明肖湘文高秀洁陈华云程钢何蕴韶
Owner DAAN GENE CO LTD
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