Polymorphism detection system and kit for gene relevant to personalized medication for cardiovascular disease

A gene detection and cardiovascular technology, applied in the fields of multiplex fluorescent PCR technology and capillary electrophoresis technology, can solve the problems of few detection sites, poor repeatability, cumbersome operation process, etc.

Active Publication Date: 2018-01-30
BEIJING MICROREAD GENE TECH
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  • Abstract
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  • Claims
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Problems solved by technology

These detection methods have defects of various degrees, such as cumbersome operation process, poor repeatability, difficul

Method used

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  • Polymorphism detection system and kit for gene relevant to personalized medication for cardiovascular disease
  • Polymorphism detection system and kit for gene relevant to personalized medication for cardiovascular disease
  • Polymorphism detection system and kit for gene relevant to personalized medication for cardiovascular disease

Examples

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[0174] Example: Detecting blood and DNA samples with a kit for detecting genetic polymorphisms related to personalized medication for cardiovascular diseases. 1. Detection system

[0175] The kit contains PCR Master Mix, positive control, negative control, and internal standard. The main components of PCR Master Mix include hot-start DNA Taq enzyme, UDG enzyme, amplification buffer, and primers for each site.

[0176] According to the allelic characteristic PCR principle, each specific primer can only be combined with the corresponding genotype DNA template and amplified. For this purpose, a series of specific changes or modifications were made to each primer. In order to coordinate amplification efficiency, improve product peak shape, and facilitate capillary electrophoresis detection, a series of specific changes or modifications have been made to the primers. The improved and optimized primer sequences used in this example are as follows:

[0177] ACE gene insertion / deletion si...

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Abstract

The invention discloses a polymorphism detection system and a kit for a gene relevant to personalized medication for a cardiovascular disease. The system relates to a quantitive fluorescent PCR (Polymerase Chain Reaction) amplification (Quantitive Fluorescent PCR, QF-PCR) technique and a capillary electrophoresis detection technique. The system has the characteristics that 1, through the multiplePCR, the amplification of 27 polymorphic sites of 17 genes is realized in a one-tube manner; 2, the reference can be provided for the use of common medicines of an antihypertensive drug, an antiplatelet drug, oral anticoagulant drug, a blood lipid regulation drug and the like by a detection result; 3, the system can be used for realizing the direct amplification of blood and blood card, and is used for avoiding the step of extracting DNA (Deoxyribonucleic Acid); 4,the system can be used for integrating a UDG-dUTP (Uracil DNA Glycosylase-Deoxyuridine Triphosphate) pollution prevention measure,and can be used for effectively preventing a product from being polluted. The polymorphism detection system and the kit have the advantages of being complete in detection site coverage, high in specificity, high in sensitivity, high in reliability, simple and convenient to operate, high in practicability and low in cost, and has large-batch detection ability.

Description

technical field [0001] The invention relates to a multiple fluorescent PCR technology and capillary electrophoresis technology. At the same time, the polymorphisms of 27 gene loci related to personalized medicine for cardiovascular diseases were detected, which provided a reference for personalized medicine in the treatment of cardiovascular-related diseases in clinical practice. It belongs to the clinical molecular diagnosis detection technology in the field of biomedicine. Background technique [0002] The incidence of cardiovascular disease is increasing year by year all over the world, and has become the number one killer affecting human health. However, due to individual differences, the therapeutic effects of the drugs commonly used in clinical practice for cardiovascular diseases are obviously different for different patients taking the same dose of drugs, which makes clinicians have certain troubles when using the drugs for treatment. More and more studies have fou...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6883C12N15/11
Inventor 张奇刘朝晖张晔周洲陈初光
Owner BEIJING MICROREAD GENE TECH
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