39 results about "Mycoplasma contamination" patented technology

The invention discloses a swine testicular cell strain ST-S suitable for suspension culture as well as an acquisition method and an application of the swine testicular cell strain ST-S. ST cells, which are subjected to adherent culture, are domesticated into ST cells which are suitable for suspension culture and can be passaged stably, and the ST cells are named as ST-S; and the ST-S cells keep sensitivity to classical swine fever virus (CSFV) and are capable of avoiding mycoplasma contamination. The invention provides a large-scale industrial suspension culture mode on the ST-S cells, and the suspension culture mode is applicable to culture by virtue of a shake flask and a bioreactor. By virtue of the ST-S cells obtained from the suspension culture, vaccines or other bio-products can be produced, so that a virus production process is simplified, product stability is enhanced, serum dosage and production cost are reduced and production efficiency is improved.

Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.

Mycoplasma contamination of a known cell line is detected by collecting a Raman spectrum of a targeted volume within a sample, the targeted volume containing a known cell line of interest, obtaining a reference spectrum uniquely associated with the known cell line where the obtained reference spectrum is known to be free of mycoplasma and comparing, using a processing device, the reference spectrum to the collected spectrum. Mycoplasma is further detected by identifying whether there are unnatural molecular compositions within the collected spectrum based upon the comparison of the reference spectrum to the collected spectrum and providing an indication as to whether mycoplasma is detected in the collected Raman spectrum based upon whether unnatural molecular compositions are identified within the collected spectrum.

The invention discloses a swine testicular cell strain ST-S suitable for suspension culture as well as an acquisition method and an application of the swine testicular cell strain ST-S. ST cells, which are subjected to adherent culture, are domesticated into ST cells which are suitable for suspension culture and can be passaged stably, and the ST cells are named as ST-S; and the ST-S cells keep sensitivity to classical swine fever virus (CSFV) and are capable of avoiding mycoplasma contamination. The invention provides a large-scale industrial suspension culture mode on the ST-S cells, and the suspension culture mode is applicable to culture by virtue of a shake flask and a bioreactor. By virtue of the ST-S cells obtained from the suspension culture, vaccines or other bio-products can be produced, so that a virus production process is simplified, product stability is enhanced, serum dosage and production cost are reduced and production efficiency is improved.

The invention provides a kind of product that can prevent the contamination in cell cultivating. The characteristic of the product is that every liter product contains 1-50g ethyl ciprofloxacin. The invention adds drug of certain density which can prevent and clear the contamination to the cell culture fluid to prevent the common contamination of bacteria and mycoplasma. The invention has overcome many defects of common drugs such as short drug action period, short shelf life, high price and unable to preserve in ambient temperature. The invention can not only be used in cell cultivating, it can also be used to clarify other organic medium materials, wash the commonly used apparatus or clarify the separating and purifying materials in emergency.

The invention provides a reagent for eliminating mycoplasma contamination in cell culture and a use method thereof. The reagent is prepared by mixing liquid A and liquid B isometrically or mixing liquid A, liquid B and liquid C isometrically, wherein the liquid A is prepared by mixing semisynthetic pleuromutilin derivatives and 0.01MPBS, and the mass-volume percentage concentration of the solute after mixing is 0.5-1.5%; the liquid B is prepared by mixing quinolone derivatives and 0.01MPBS, and the mass-volume percentage concentration of the solute after mixing is 0.5-1.5%; and the liquid C is prepared by mixing tetracycline derivatives and 0.01MPBS, and the mass-volume percentage concentration of the solute after mixing is 0.25-0.75%. The reagent and method provided by the invention have the following beneficial effects: if contamination is not severe, the effect can be displayed within a week; and after the treatment cycle is finished, black spots do not exist among the treated contaminated cells, the growth cycles of the cells return to normal, the cell morphology is normal, and aggregated growth and netting phenomena are avoided.