The invention discloses an LAMP primer group for detecting broad-spectrum mycoplasmas. The LAMP primer group consists of an outer primer F3, an outer primer B3, an inner primer FIP, an inner primer BIP, a loop primer LF and a loop primer LB, wherein the primers are respectively shown as SEQ ID NO.1-6. The invention further discloses a mycoplasma visible detection kit with the primer group, and application of the mycoplasma visible detection kit. The kit disclosed by the invention has advantages of good mycoplasma specificity, sensitivity, rapid and efficient amplification, visible identification and the like. By adoption of the detection system disclosed by the invention, normal mycoplasma contamination can be rapidly and sensitively detected in the cell culture process under an isothermal condition of 60-62 DEG C, no complex instrument is needed, and mycoplasma contamination detection in a cell laboratory at regular time can be relatively well achieved.