Reagent for eliminating mycoplasma contamination in cell culture and use method thereof

Abstract

The invention provides a reagent for eliminating mycoplasma contamination in cell culture and a use method thereof. The reagent is prepared by mixing liquid A and liquid B isometrically or mixing liquid A, liquid B and liquid C isometrically, wherein the liquid A is prepared by mixing semisynthetic pleuromutilin derivatives and 0.01MPBS, and the mass-volume percentage concentration of the solute after mixing is 0.5-1.5%; the liquid B is prepared by mixing quinolone derivatives and 0.01MPBS, and the mass-volume percentage concentration of the solute after mixing is 0.5-1.5%; and the liquid C is prepared by mixing tetracycline derivatives and 0.01MPBS, and the mass-volume percentage concentration of the solute after mixing is 0.25-0.75%. The reagent and method provided by the invention have the following beneficial effects: if contamination is not severe, the effect can be displayed within a week; and after the treatment cycle is finished, black spots do not exist among the treated contaminated cells, the growth cycles of the cells return to normal, the cell morphology is normal, and aggregated growth and netting phenomena are avoided.

Description

technical field [0001] The invention relates to a reagent for removing mycoplasma contamination in cell culture and a method for using the same. Background technique [0002] Mycoplasma is the most common pollutant in cell culture, which will interfere with the experimental results. However, since the binary division time of mycoplasma is 1-6 hours, it is difficult to detect the initial contamination of cultured cells by mycoplasma. Common contaminating mycoplasmas in cell cultures were derived from M. orale, M. arginini, M. hyorhinis and A. laidlawii. After the cultured cells are contaminated with mycoplasma, they will have different pathogenicity according to the antigenicity and biochemical metabolism of different mycoplasmas, but the general performance is the same. At first, the experimenter noticed that the cultured cells grew slowly, and then the culture medium was very slow. It will soon turn yellow, and the cell growth cycle will be prolonged, and then a large numb...

AI Technical Summary

Problems solved by technology

Because the classification of mycoplasma is different from that of bacteria, the antibiotics generally used for bacteria cannot kill and inhibit mycoplasma very well. Due to the variety of mycoplasma, it is necessary to use targeted broad-spectrum antibiotics in combination to achieve maximum clearance and inhibition. , but no reagents and methods that can systematically eliminate mycoplasma contamination have been developed

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