Composition for efficiently removing mycoplasma contamination in cell culture process

Abstract

The invention discloses a composition for efficiently removing mycoplasma contamination in a cell culture process. The composition is composed of antibacterial peptides BMAP-18 and BMAP-18-R9, whereinthe BMAP-18 has the function of killing mycoplasmas outside cells, and the BMAP-18-R9 enters the cells under the effect of R9 cell-penetrating peptides to kill the mycoplasmas in the cells. Through the combination of the BMAP-18 and the BMAP-18-R9, the mycoplasmas inside and outside the cells can be thoroughly killed. In this way, the BMAP-18 and the BMAP-18-R9 can be used separately or combinedly, and no toxic effect is caused on the cells when the peptides are within the concentration of 200 micromoles.

Description

technical field [0001] The invention belongs to the field of life sciences, and in particular relates to a formula for removing mycoplasma pollution in the cell culture process. Background technique [0002] Mycoplasma is a prokaryotic organism with a size of only 0.2-0.3 um, no cell wall, and can pass through a general filter membrane (0.22-0.45um). In the process of cell culture, the incidence of mycoplasma infection reaches 63%. Contamination by mycoplasma is a worldwide problem. When cells (especially passage cells) are contaminated by mycoplasma, the expression of DNA, RNA and protein in the cells will change, but the growth rate of the cells generally does not have a significant impact, so the contamination of cells by mycoplasma is generally difficult to detect, eventually, leading to There are serious problems such as poor reproducibility of experimental results. [0003] At present, it has been reported that BMAP-18 is an antimicrobial peptide that can clear commo...

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Problems solved by technology

Through experiments, we found that BMAP-18 has a certain removal effect on mycoplasma contaminated by cell cul

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