Reagent, kit and treatment for treating mycoplasma contamination
A kit and mycoplasma technology are applied in the field of reagents for treating mycoplasma contamination, which can solve the problems of reliability discount and loss of research results, and achieve the effects of safe, fast and effective treatment, safe use, and reduced damage.
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Embodiment 1
[0018] Embodiment 1: the preparation of the reagent that handles mycoplasma pollution and kit
[0019] Take the 10ml kit as an example:
[0020] Dissolve 200mg tiamulin, 50mg minocycline and 150mg antibiotic ciprofloxacin in 10ml sterile double-distilled water in turn, and mix thoroughly to obtain the prepared reagent for treating mycoplasma contamination; then, the reagent Assembled into kit.
Embodiment 2
[0021] Embodiment 2: the method for processing mycoplasma pollution
[0022] The reagent prepared in Example 1 (or the reagent in the kit) is added to fresh RMPI 1640 medium according to the volume ratio of 1:100 (that is, the diluted volume is 100 times the volume of the reagent in Example 1) (The cells to be treated are 293T cells, which adopt RMPI 1640 medium) to obtain the antibiotic culture medium, wherein the concentration of the antibiotic tiamulin is 0.2mg / ml, the concentration of minocycline is 0.05mg / ml, and the antibiotic ring The concentration of profloxacin was 0.15mg / ml.
[0023] Add the cell culture to be treated to the above-mentioned antibiotic medium, and after culturing for 3 days, discard the old medium; replace with a new antibiotic medium, and culture for 3 days; and so on, until no mycoplasma contamination is detected.
[0024] After no mycoplasma contamination can be detected, normal cell culture or subculture can be carried out.
[0025] In this em...
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