Reagent, kit and treatment for treating mycoplasma contamination

A kit and mycoplasma technology are applied in the field of reagents for treating mycoplasma contamination, which can solve the problems of reliability discount and loss of research results, and achieve the effects of safe, fast and effective treatment, safe use, and reduced damage.

Inactive Publication Date: 2016-10-26
SHANGHAI XP BIOMED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For relevant scientific researchers, the most frightening thing is that mycoplasma pollution changes man

Method used

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  • Reagent, kit and treatment for treating mycoplasma contamination
  • Reagent, kit and treatment for treating mycoplasma contamination
  • Reagent, kit and treatment for treating mycoplasma contamination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation of the reagent that handles mycoplasma pollution and kit

[0019] Take the 10ml kit as an example:

[0020] Dissolve 200mg tiamulin, 50mg minocycline and 150mg antibiotic ciprofloxacin in 10ml sterile double-distilled water in turn, and mix thoroughly to obtain the prepared reagent for treating mycoplasma contamination; then, the reagent Assembled into kit.

Embodiment 2

[0021] Embodiment 2: the method for processing mycoplasma pollution

[0022] The reagent prepared in Example 1 (or the reagent in the kit) is added to fresh RMPI 1640 medium according to the volume ratio of 1:100 (that is, the diluted volume is 100 times the volume of the reagent in Example 1) (The cells to be treated are 293T cells, which adopt RMPI 1640 medium) to obtain the antibiotic culture medium, wherein the concentration of the antibiotic tiamulin is 0.2mg / ml, the concentration of minocycline is 0.05mg / ml, and the antibiotic ring The concentration of profloxacin was 0.15mg / ml.

[0023] Add the cell culture to be treated to the above-mentioned antibiotic medium, and after culturing for 3 days, discard the old medium; replace with a new antibiotic medium, and culture for 3 days; and so on, until no mycoplasma contamination is detected.

[0024] After no mycoplasma contamination can be detected, normal cell culture or subculture can be carried out.

[0025] In this em...

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Abstract

The present invention discloses a reagent, a kit and a treatment method for treating mycoplasma contamination. The reagent is a solution containing antibiotic tiamulin, minocycline and antibiotic ciprofloxacin, wherein a concentration ratio of the antibiotic tiamulin to the minocycline to the antibiotic ciprofloxacin is 4:1:3. According to the present invention, with the reagent, the kit and the treatment method, the combination comprising the antibiotic tiamulin, the minocycline and the antibiotic ciprofloxacin according to the specific ratio is used, such that the mycoplasma contamination treatment effect can be significantly increased; and the mycoplasma contamination problem can be simply, safely, rapidly and effectively treated.

Description

technical field [0001] The invention relates to a reagent for treating mycoplasma pollution, a kit thereof and a treatment method, belonging to the field of biotechnology. Background technique [0002] Mycoplasma (Mycoplasma) pollution is one of the most common pollutions in the cell culture process, especially in the culture process of mammalian cells, the problem of mycoplasma pollution is particularly troublesome. The main reasons are the following three points: 1) Mycoplasma is very small in size, The diameter is 0.1 μm, which cannot be removed by general filter sterilization, and its morphological structure is difficult to see under an optical microscope; 2) Mycoplasma is tenacious, can survive in alkaline conditions (pH 7.6-8.0), and is resistant to penicillin; 3) At the initial stage of cells being contaminated by mycoplasma, some sensitive cells can see that the growth and proliferation of the cells slow down and the shape becomes round. However, most cells have no ...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0602C12N2500/30
Inventor 张智培熊延狮
Owner SHANGHAI XP BIOMED
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