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Product for prevention and control of pollution of cell culture

A cell culture and product technology, applied in the field of biological sciences, can solve the problems of narrow antibacterial spectrum of double antibodies, inability to store at room temperature, and short drug effect time.

Inactive Publication Date: 2007-04-25
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antibacterial spectrum of double antibodies is narrow, and the drug effect time is extremely short under cell culture conditions, making it difficult to maintain long-term cell culture
In recent years, a new antibacterial drug has been introduced abroad, its name is Plasmocin, which can prevent and remove common pollution in cell culture, but the drug is extremely expensive and cannot be stored at room temperature, causing many The unit is unaffordable and abandoned

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Mix and dissolve the following components into 1 liter of aqueous solution, adjust the pH value to 7.4 after mixing thoroughly, then filter and sterilize, and obtain this product after packaging:

[0040] KCl 0.15g

[0041] K H 2 PO 4 0.15 grams

[0042] NaCl 6.00 g

[0043] Na 2 HPO 4 0.86 grams

[0044] Ethyl Ciprofloxacin 1.0g.

Embodiment 2

[0046] Mix and dissolve the following components into 1 liter of aqueous solution, adjust the pH value to 7.4 after mixing thoroughly, then filter and sterilize, and obtain this product after packaging:

[0047] KCl 0.20g

[0048] K H 2 PO 4 0.20 grams

[0049] NaCl 8.00 g

[0050] Na 2 HPO 4 1.14 grams

[0051] Ethyl Ciprofloxacin 5.0g.

Embodiment 3

[0053] Mix and dissolve the following components into 1 liter of aqueous solution, adjust the pH value to 7.4 after mixing thoroughly, then filter and sterilize, and obtain this product after packaging:

[0054] KCl 0.25g

[0055] K H 2 PO 4 0.25 grams

[0056] NaCl 10.00 g

[0057] Na 2 HPO 4 1.43 grams

[0058] Ethyl Ciprofloxacin 50.0g.

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Abstract

The invention provides a kind of product that can prevent the contamination in cell cultivating. The characteristic of the product is that every liter product contains 1-50g ethyl ciprofloxacin. The invention adds drug of certain density which can prevent and clear the contamination to the cell culture fluid to prevent the common contamination of bacteria and mycoplasma. The invention has overcome many defects of common drugs such as short drug action period, short shelf life, high price and unable to preserve in ambient temperature. The invention can not only be used in cell cultivating, it can also be used to clarify other organic medium materials, wash the commonly used apparatus or clarify the separating and purifying materials in emergency.

Description

technical field [0001] The invention relates to a product for preventing and controlling cell culture pollution, belonging to the technical field of biological sciences. Background technique [0002] The bioeconomy is a new economy in the 21st century, and the bioindustry supported by biotechnology has become a new high-tech industry and an important part of the national economy. Cell culture is the basic technology of cell engineering in biotechnology. The quality of cells directly affects the accuracy of scientific research results and the quality and safety of biological products. The prevention and control of pollution in cell culture has become a major topic in the field of biotechnology. In existing cell culture, in order to prevent and control cell contamination, the most commonly used method is to use antibiotics. Commonly used antibiotics include penicillin, streptomycin, kanamycin, etc., among which penicillin and streptomycin are the most used, namely double an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00A61L2/16
Inventor 张念祖李志华高林
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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