A processing method for contaminated suspension cultured cell

A technology for suspension culture cells and treatment methods, which is applied in the field of treatment after suspension culture cells are polluted, and can solve problems such as cell impurity and cell cross-contamination, and achieve good treatment effects

Inactive Publication Date: 2018-03-09
金时代进出口贸易重庆有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

In addition, as the types of cells used increase, different cells are cross-contaminated, resulting in

Method used

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Embodiment 1

[0018] A treatment method after contamination of suspension culture cells, the specific steps of the treatment method after contamination of suspension culture cells are as follows:

[0019] S1. Identification of pollution types: 1. Identification of fungi. There are many types of fungi with different shapes, but they are easy to find after contamination. Most of them are white or light yellow dots floating on the surface of the culture medium, which can be seen with the naked eye; some are scattered and grown under a microscope. It can be seen that it is filamentous, tubular, and dendritic, criss-crossing between cells; 2. Mycoplasma identification. After mycoplasma contaminates cells, the cells will not change significantly, and can be identified by observing cell growth and PCR detection; 3. Bacteria For identification, take 5ml of suspected contaminated stem cell suspension and centrifuge at 800-1000r / min for 5-7 minutes, add 5ml of sterile bacterial culture solution withou...

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Abstract

The invention discloses a method for treating contamination of suspension culture cells. The specific steps of the treatment method for suspension culture cells after contamination are as follows: S1, identification of pollution types, S2, treatment of fungal pollution, S3 treatment of mycoplasma pollution, S4 Bacterial contamination treatment. The method first distinguishes different pollution categories through different identification methods, and then treats different pollution sources separately. Compared with the traditional treatment method after cell pollution occurs, the present invention is simple and easy to implement, and has better treatment effect.

Description

technical field [0001] The invention belongs to the field of cell culture, and more specifically relates to a treatment method for contamination of suspension culture cells. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells, pluripotent stem cells, and unipotent stem cells (multipotent stem cells). Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. There are many ways to extract it: if it is hematopoietic stem cells or bone marrow mesenchymal stem cells, the most commonly u...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/0081
Inventor 宋彩霞黄启程王仁杰邱阳唐玲李国炎
Owner 金时代进出口贸易重庆有限公司
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