7results about How to "Avoid false negative results" patented technology

The invention belongs to the technical field of biological genes, and discloses a method for identifying RNA interaction protein based on a Turbo ID technology and an MCP / MS2 system, the method can be used for identifying protein interacting with RNA in vitro or in vivo, and the method specifically comprises the following steps: firstly, respectively constructing RNA expression vectors inserted with MS2 tags, then constructing fusion protein vectors expressing MCP-Turbo ID, and finally constructing fusion protein vectors for expressing MCP-Turbo ID; the method comprises the following steps of: inserting RNA (Ribonucleic Acid) into an MS2 tag, transgenosis into a plant receptor by utilizing an agrobacterium tumefaciens mediated genetic transformation method to obtain a transgenic plant material expressing MCP-Turbo ID, combining the RNA inserted with the MS2 tag with the MCP-Turbo ID in vivo / in vitro by utilizing a hybridization or in-vitro incubation method, further enabling the Turbo ID to be adjacent to the RNA, biotinylating the RNA binding protein, and obtaining a transgenic plant material expressing the MCP-Turbo ID. And then enriching biotinylated protein by adopting streptavidin magnetic beads, and identifying and analyzing through Western Blot and mass spectrometry. The method not only solves the problem that the interaction protein of specific RNA in a living cell environment cannot be effectively identified in the prior art, but also can capture a transient or low-affinity RNA-protein interaction relationship.

This disclosure belongs to the field of biotechnology, specifically relating to anti-P[8] genotype rotavirus antibodies or their antigen-binding fragments, their preparation methods, and applications. The antibody or its antigen-binding fragment can specifically recognize and bind to the VP8 protein of P[8] genotype rotavirus, and can be used for the diagnosis, prevention, and / or treatment of P[8] genotype rotavirus infection or diseases caused by it, the detection of the presence or level of P[8] genotype rotavirus, its VP8 protein, or fusion proteins containing its VP8 protein in samples, or the development or screening of drugs for the prevention and / or treatment of P[8] genotype rotavirus infection or diseases caused by it.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The invention provides a Parkinson's disease rapid RT-QuIC diagnostic kit based on A30P mutant alpha-synuclein, and belongs to the technical field of detection kits. The specific A30P mutant protein is used as a reaction substrate, and rapid and high-sensitivity amplification detection can be carried out on trace misfolded alpha-synuclein in a skin sample through an optimized RT-QuIC system. The kit has the characteristics of short detection period and convenience in operation, provides a new tool for molecular diagnosis of Parkinson's disease, is suitable for definite diagnosis of clinical PD suspected cases, and can also be used for screening high-risk groups. The kit has the advantages of mature preparation process, easily available raw materials, easiness in industrial production and large-scale popularization, and important clinical application value.

The invention discloses a totally-enclosed avian influenza H5, H7, H9 and universal virus quadruple detection kit. The kit comprises primers and probes of a universal avian influenza virus M gene, an H5 subtype avian influenza virus HA gene, an H7 subtype avian influenza virus HA gene, an H9 subtype avian influenza virus HA gene and a reference gene LconR. According to the invention, the ligand-dependent nuclear receptor corepressor protein is introduced as a reference gene, and the gene widely exists in mammals and birds and is very suitable for detection of multi-species infection epidemic diseases such as avian influenza. The internal reference can participate in nucleic acid extraction and amplification processes, strictly controls the quality of each detection step, fundamentally avoids false negative results, and significantly improves the reliability of detection results. The internal reference also expands the species application range of the detection method, so that avian influenza detection is not limited to poultry any more, and the method can be widely applied to all mammals possibly infected with avian influenza.

The invention belongs to the technical field of biology, and particularly relates to an anti-novel coronavirus antibody or an antigen binding fragment thereof as well as a preparation method and application thereof. The antibody or the antigen binding fragment of the antibody can specifically recognize and bind the Omicro BA.4 / 5 type novel coronavirus or the RBD protein of the Omicro BA.4 / 5 type novel coronavirus, and has no cross reaction on wild type SARS-CoV-2, Delta (B.1.617.2), Omicro BA.1 or the RBD protein of the Omicro BA.1; the recombinant plasmid provided by the invention has good neutralizing activity on Omicro BA.4 / 5 type new coronavirus, Omicro XBB.1 type new coronavirus and / or Omicro BQ.1.1 type new coronavirus; the compound can be used for diagnosing, preventing and / or treating novel coronavirus infection or diseases caused by the novel coronavirus infection, detecting existence or level of the novel coronavirus or RBD protein of the novel coronavirus in a sample or developing or screening drugs for preventing and / or treating the novel coronavirus infection or the diseases caused by the novel coronavirus infection.

This invention discloses a positive control for mycoplasma detected by quantitative real-time PCR (qPCR) that can distinguish between positive control contamination and positive control contamination, along with its applications. The invention provides a positive control obtained by inserting an irrelevant sequence into a highly conserved region of mycoplasma. Using this positive control, an internal probe is designed to distinguish between positive samples and the positive control, avoiding potential cross-contamination and establishing a method for detecting mycoplasma that can distinguish between positive control contamination and positive control contamination. This method can determine the accuracy of qPCR results, eliminate interference from positive control contamination of the test sample, and is highly sensitive with a wide detection range. It can be used for detecting mycoplasma contamination in scientific research and the biopharmaceutical industry.