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Fluorescence immunochromatography device for detecting COVID-19 and using method thereof

A fluorescent immunochromatography and coronavirus technology, which is applied in measuring devices, immunoassays, analytical materials, etc., can solve problems such as high technical requirements, inability to apply early preliminary screening, and long detection time for new coronaviruses, and meet personnel requirements low, improve the accuracy of inspection, and avoid false negative results

Inactive Publication Date: 2020-04-24
SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The nucleic acid detection of the new coronavirus COVID-19 has high specificity and sensitivity, but the detection method has high technical requirements, the specimen needs special treatment, and requires professional equipment such as PCR amplification instrument and gel electrophoresis. Virus detection takes a long time, requires professional technicians to operate and judge the test results, and cannot be applied to early preliminary screening at grassroots level such as community hospitals, customs, and CDC

Method used

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  • Fluorescence immunochromatography device for detecting COVID-19 and using method thereof
  • Fluorescence immunochromatography device for detecting COVID-19 and using method thereof
  • Fluorescence immunochromatography device for detecting COVID-19 and using method thereof

Examples

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Embodiment 1

[0080] A fluorescent immunochromatographic device for detecting novel coronavirus COVID-19, including a test strip.

[0081] The test strip includes a bottom plate and a sample pad, a binding pad, a reaction pad and a water-absorbing pad that are connected in sequence above the bottom plate; the new coronavirus NP protein monoclonal antibody labeled with europium microspheres and europium are coated on the binding pad. Microsphere-labeled rabbit IgG antibody, the particle size of the europium microspheres used in the europium microsphere labeling is 610nm; the mass concentration ratio between the novel coronavirus NP protein monoclonal antibody and rabbit IgG antibody is 1.5 : 1; the reaction pad is provided with a detection line and a quality control line successively along the sample flow direction, and the detection line is coated with a new type of coronavirus NP protein monoclonal antibody, and the quality control line is coated with a goat anti-rabbit IgG antibodies.

...

Embodiment 2

[0087] A fluorescent immunochromatographic device for detecting novel coronavirus COVID-19, including a test strip and a sample diluent.

[0088] The test strip includes a bottom plate and a sample pad, a binding pad, a reaction pad and a water-absorbing pad that are connected in sequence above the bottom plate; the new coronavirus NP protein monoclonal antibody labeled with europium microspheres and europium are coated on the binding pad. Microsphere-labeled rabbit IgG antibody, the particle size of the europium microspheres used in the europium microsphere labeling is 600nm, and the mass concentration ratio between the novel coronavirus NP protein monoclonal antibody and the rabbit IgG antibody is 2 : 1; the reaction pad is sequentially provided with a detection line and a quality control line along the flow direction of the sample, the detection line is coated with a new type of coronavirus NP protein monoclonal antibody, and the quality control line is coated with a goat an...

Embodiment 3

[0096] A fluorescent immunochromatographic device for detecting novel coronavirus COVID-19, including a test strip and a sample diluent.

[0097] The test strip includes a bottom plate and a sample pad, a binding pad, a reaction pad and a water-absorbing pad that are connected in sequence above the bottom plate; the new coronavirus NP protein monoclonal antibody labeled with europium microspheres and europium are coated on the binding pad Microsphere-labeled rabbit IgG antibody, the particle size of the europium microspheres used in the europium microsphere labeling is 550nm, and the mass concentration ratio between the novel coronavirus NP protein monoclonal antibody and the rabbit IgG antibody is 1 : 1; the reaction pad is sequentially provided with a detection line and a quality control line along the flow direction of the sample, the detection line is coated with a new type of coronavirus NP protein monoclonal antibody, and the quality control line is coated with a goat ant...

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Abstract

The invention discloses a fluorescence immunochromatography device for detecting a novel coronavirus COVID-19 and a using method of the fluorescence immunochromatography device. The fluorescence immunochromatography device disclosed by the invention is high in sensitivity and strong in specificity, the accuracy is high, the detection sensitivity can reach 10pg / ml, the detection speed is high, theoperation is simple, the device is portable, the requirement for personnel is low, the operation of professionals is not needed, the detection cost is low, the device can be applied to preliminary screening of various places such as hospitals, airports, customs and disease control centers, the detection time (10-15 min) is short, a simpler, more convenient and faster field detection means is provided for suspected patient investigation and asymptomatic infected person screening, and therefore epidemic spreading is prevented as soon as possible.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a fluorescent immunochromatographic device for detecting novel coronavirus COVID-19 and its application method. Background technique [0002] In mid-December 2019, atypical pneumonia of unknown etiology was first discovered in Wuhan, China, and scientific research showed that it was caused by a new type of coronavirus, COVID-19. Pneumonia caused by the novel coronavirus COVID-19 is mainly characterized by fever, fatigue, and dry cough, and a small number of patients are accompanied by upper respiratory and gastrointestinal symptoms such as nasal congestion, runny nose, and diarrhea. Severe cases often develop dyspnea one week later, and severe cases rapidly progress to acute respiratory distress syndrome, septic shock, difficult-to-correct metabolic acidosis, and coagulation dysfunction. So far, there is no specific drug for COVID-19. The therapeutic drugs reported...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/577G01N33/569G01N33/558
CPCG01N33/558G01N33/56983G01N33/577G01N33/582G01N2333/165G01N2469/10
Inventor 周宏伟吴玉章温坤陈嘉慧王佑春赵晨燕黄维金梁昊宇聂建辉颜文豪陈奕耀陈立许稳健禄亚峰潘晓妹王云燕赵晋冯舒婷袁克湖魏世塨
Owner SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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