Respiratory tract infection pathogen nucleic acid combined detection kit

Abstract

The invention discloses a respiratory tract infection pathogen nucleic acid combined detection kit. The invention develops a primer and probe combination for detecting various respiratory tract infection pathogens such as novel corona virus, influenza A virus, influenza B virus, respiratory syncytial virus, human parainfluenza virus, adenovirus, mycoplasma pneumoniae and chlamydia pneumoniae by combining a multiple fluorescent quantitative PCR technology and a diversion hybridization gene chip technology. The nucleotide sequences of the primer and probe combination are shown in SEQ ID NO: 1-36in sequence. The respiratory tract infection pathogen nucleic acid combined detection kit is constructed. Synchronous joint detection of eight respiratory tract infection pathogens can be realized, the detection accuracy is good, the specificity is strong, the sensitivity is high, the repeatability is good, false negative and false positive are low, the detection time is short, the cost is low, apatient can be comprehensively detected, the pathogens can be accurately positioned, timely treatment is carried out or corresponding isolation measures are carried out, and the kit has important significance for effectively controlling respiratory tract infection to prevent related infectious infection outbreak.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and clinical diagnosis. More specifically, it relates to a nucleic acid combined detection kit for respiratory infection pathogens. Background technique [0002] Respiratory tract infection (Respiratory tract infection, RTI) is the most common type of disease in humans, which can occur in any gender, age and region, and is one of the most important causes of morbidity and mortality worldwide. The clinical symptoms and signs caused by respiratory tract infection are relatively similar, and its clinical manifestations are mainly rhinitis, pharyngitis, laryngitis, tonsillitis and other symptoms, and severe cases can cause tracheitis, bronchitis and pneumonia, etc. Treatment methods, efficacy, and course of disease vary. It has been proven that most respiratory diseases are caused by pathogens other than bacteria, among which respiratory viruses are the most common. [0003] Common respiratory ...

AI Technical Summary

Problems solved by technology

However, in the clinical detection of respiratory tract infection, single detection of a pathogen of respiratory tract infection is time-consuming, laborious and costly.

At present, multiple detection is mainly based on the antigen or antibody detection of immunological methods, but the sensitivity and specificity of immunological methods are low, and there is a window period in antibody detection, which is easy to miss in the early diagnosis of diseases

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