Detection chip, detection kit and detection method for bioterrorism

Abstract

The invention relates to a detection chip, a detection kit and a detection method for bioterrorism factors. Probe molecules and PCR amplification primers are designed according to the gene conservation regions of various bioterrorism factors, and multiple probe molecules are integrated into one detection method. On the chip, the detection throughput is high. At the same time, due to the application of gene chip detection, the detection sensitivity is high and the speed is fast; according to the analysis of the designed PCR amplification primer sequence, multiple PCR technology can be used to detect nucleic acid substances of various bioterrorism factors at one time. Amplification, thereby further speeding up the detection speed.

Description

【Technical field】 [0001] The invention relates to the field of biomolecular detection, in particular to a detection chip, a detection kit and a detection method for bioterrorism factors. 【Background technique】 [0002] The traditional detection of important bioterrorism factors (such as Bacillus anthrax, Lassa virus, Clostridium botulinum toxin, Brucella, etc.) is still in the routine biochemical culture stage. Factor screening will appear helpless; similarly, real-time fluorescent PCR technology can usually only amplify one or at most several bioterrorism factors at the same time, and when faced with a sample containing multiple or even dozens of bioterrorism factors to be detected , it is necessary to carry out multiple PCR reactions to amplify all the bioterrorism factors in the sample to achieve the detection purpose, which makes the experimental operation not only cumbersome and time-consuming, but also causes a great waste of experimental consumables, and cannot quickl...

AI Technical Summary

Problems solved by technology

[0002] The traditional detection of important bioterrorism factors (such as Bacillus anthrax, Lassa virus, Clostridium botulinum toxin, Brucella, etc.) is still in the routine biochemical culture stage. Factor screening will appear helpless; similarly, real-time fluorescent PCR technology can usually only amplify one or at most several bioterrorism factors at the same time, and when faced with a sample containing multiple or even dozens of bioterrorism factors to be detected , it is necessary to carry out multiple PCR reactions to amplify all the bioterrorism factors in the sample to achieve the detection purpose, which makes the experimental operation not only cumbersome and time-consuming, but also causes a great waste of experimental consumables, and cannot quickly detect the bioterrorism factors. Make screening and make timely judgments, and it is easy to lose the best time to make accurate analysis of bioterrorism factors in samples

Images