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Automatic device for evaluating memory function of primate experimental animal
ActiveCN104663490AIncrease motivation to eatAvoid interferenceTaming and training devicesDiseaseBrain function
The invention discloses an automatic device for evaluating the memory function of a primate experimental animal and a method for evaluating the memory function of the primate experimental animal. The device comprises an experimental operation cage (1), a touch display screen (15), a horizontal exhibition stand (12), an infrared ray sensing automatic award food distributor (16) and a main control computer (19). Through interactive operation of the touch display screen and the animal, direct interactive operation between experiment personnel and the animal is avoided, and the evaluation of the cognitive ability is more objective. Through the device, the brain function can be evaluated subjectively according to a judgment made by a tester. The device and the method are particularly suitable for evaluating the cognitive memory of primate animals, particularly old non-human primate experimental animals and disease model non-human primate experimental animals.
Owner:广西南宁灵康赛诺科生物科技有限公司
System and method for detecting performance of mobile electronic tags
InactiveCN103279774AMeet the requirements of personalized customizationImprove mobilityTicket-issuing apparatusTesting sensing arrangementsPersonalizationSoftware engineering
The invention discloses a system and method for detecting the performance of mobile electronic tags. The system comprises a testing device, radio-frequency identification electronic tags to be tested and testing equipment, wherein the radio-frequency identification electronic tags to be tested pass through a testing passage; the testing device consists of a mobile trolley and the testing passage; the radio-frequency identification electronic tags to be tested are stuck to objects; and the testing equipment comprises a computer, a read-write device and a read-write device antenna device. The objects with the electronic tags to be tested can pass through the testing passage through the mobile trolley, and a read-write device antenna is mounted in the testing passage; and the positions / states of the read-write device and the antenna and the speed of the mobile trolley are adjusted, and the read-write device reads the electronic tags on the objects through the antenna, so that the optimal electronic tag reading rate can be obtained through repeated testing. Through the system and the method, disclosed by the invention, the position / direction of the antenna and the moving speed of the objects are subjected to individualized customization on the premise that the electronic tags on fixed sticking positions of the objects are subjected to 100% reading, so that a reference is provided for the use of the electronic tags in manufacturing production lines and logistics.
Owner:INST OF AUTOMATION CHINESE ACAD OF SCI
Temperature control method and device for polymerase chain reaction biochip
InactiveCN103135634AReduction of biological actual useImprove reaction efficiencyTemperatue controlData acquisitionEngineering
The invention provides a temperature control method and a device for a polymerase chain reaction biochip. The temperature control device for the polymerase chain reaction biochip comprises a power module, a temperature collecting module, a signal amplifying module, a data collecting card module, a chip heating module and a chip cooling module. The temperature collecting module converts a resistance signal of a platinum temperature sensor on the biochip into a voltage signal through the stimulating of a power supply. The signal is inserted into a Lab VIEW signal colleting card after amplification and noise filtering. The collecting card possesses an A / D conversion function and a signal conditioning function, and then the digital voltage signal enters a LABVIEW temperature control program. An analog control signal is output through the data collecting card, the temperature of the chip is enabled to meet a reaction requirement by starting a chip heating or cooling module. A temperature control curve is displayed on a PC (personal computer) display screen in real time in a reaction process. The device provided is capable of achieving loosed-loop automatic temperature control, good in heating and cooling processes, good in stability and high in human-machine interactivity. The temperature control method and the device for a polymerase chain reaction biochip are a novel detection analysis method and novel detection analysis equipment.
Owner:SHANGHAI JIAO TONG UNIV
Experimental device for underground two-dimensional elevation sandiness mud flat petroleum pollution
InactiveCN103234874AFlexible controlFlexible adjustment performanceSurface/boundary effectManufacturing technologyPetroleum Pollution
The invention belongs to an equipment manufacturing technology in the field of beach petroleum pollution and remediation, and relates to an experimental device for underground two-dimensional elevation sandiness mud flat petroleum pollution. Various pressure measurement tubes arranged on a pressure measurement raft are connected with sand soil sampling holes; seawater level measurement holes are communicated and connected with the pressure measurement tubes in the pressure measurement raft; a seawater storage tank evacuation tube valve is arranged on one side of the bottom of a seawater storage tank; the upstream tank bottom of a first overflow partition plate arranged in the middle of a seawater level adjustment tank is connected with a water inlet hose; the downstream tank bottom is connected with an overflow tube; a first self-locking gear rack elevator is arranged on the left side face of an elevation steel framework tank body; the upstream tank bottom of a second overflow partition plate arranged in the middle of an underground water level adjustment tank is connected with an overflow region water outlet end vertical shaft; the downstream tank bottom is connected with an underground percolation water outlet overflow tube; a gravimetric method measurement water tank is placed on an electronic platform scale; and the lower end of a wave paddle is fixedly mounted on a bearing fixed bracket on the inner side of a left elevation dismountable door plate. The device is reasonable in structure, simple to operate, easy to manufacture and grasp, and flexible to control and adjust.
Owner:QINGDAO TECHNOLOGICAL UNIVERSITY
Simulation experiment method for tunnel drivage blasting
The invention discloses a simulation experiment method for tunnel drivage blasting. The simulation experiment method includes the steps of: (1) manufacturing a simulation blasting experiment device, (2) utilizing the blasting experiment device to simulate tunnel cutting blasting, (3) utilizing millisecond initiation technology to perform blasting experiment, and (4) studying cutting holes, auxiliary holes, peripheral hole blasting rock breaking mechanisms, rock movement characteristics after the blasting and blasting effects. According to the simulation experiment method tunnel drivage blasting, a size design and a blasting parameter calculation design are performed through a law of similitude and a corresponding ratio of similitude and a blasting network is arranged, tunnel drivage blasting processes of various kinds of sections and processes of rock breaking and slag throwing of cutting drivage blasting are more completely and truly simulated, and a speed of blasting slag leaving cutting chamber is calculated according to law of motion of blasting slag. Accordingly, a more reliable experiment method and a more reliable experiment result are provided for a mechanism study of mine tunnel drivage blastings.
Owner:SHANDONG UNIV OF SCI & TECH
Device for intravenous injection of mouse tail and superficial intravenous injection of other parts
InactiveCN104799968AEasy to fixEnsure safetyDiagnostic recording/measuringSensorsVein injectionClosed cavity
The invention relates to a device for intravenous injection of a mouse tail and superficial intravenous injection of other parts. The device comprises a base, a transparent tube, a front fixing part and a rear fixing part, wherein a notch is formed in the rear fixing part, and ventilation holes are formed in two side edges of the transparent tube; the base is a closed cavity with a light source inside, the base comprises a top plate, a soleplate and four side plates, an adjustable light-transmitting operation panel which is hinged to the top plate is arranged between the side plate facing one end of the tail part of a mouse and the top plate, the side edge of the top plate is provided with a fixing plate facing the interior of the base, the adjustable light-transmitting operation panel is provided with an angle adjusting screw for adjusting an angle between the adjustable light-transmitting operation panel and the fixing plate, and other three side edges of the adjustable light-transmitting operation panel are respectively provided with an elastic light screening plate which is connected to the side edge of the adjacent base. By adopting the device, the safety of the operator is adequately guaranteed, and the operator can be prevented from being bitten by the mouse when in injection; moreover, the mouse is firmly fixed, the stress response of the mouse can be prevented from affecting the experiment result, and the experiment result is more reliable.
Owner:THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF SCI & TECH
Shale gas well simulated reservoir fracture fracturing liquid flowback rate experimental measurement device and method
ActiveCN109490507AExperimental results are reliableImprove guidanceEarth material testingMaterial weighingMeasurement deviceFracturing fluid
The invention discloses a shale gas well simulated reservoir fracture fracturing liquid flowback rate experimental measurement device and method. The shale gas well simulated reservoir fracture fracturing liquid flowback rate experimental measurement device comprises a clamp, a press machine, a thermostat, a constant-speed and constant-pressure pump, an intermediate container, a constant-speed andconstant-pressure pump outlet valve, a fluid inlet valve, a fluid outlet valve, a nitrogen cylinder outlet valve, a nitrogen cylinder and an outlet valve. The method mainly comprises the steps of 1)rock sample preparation, 2) shale physical property parameter testing, 3) testing parameter calculation, 4) fracturing liquid preparation and density testing, 5) testing parameter testing, and 6) shale flowback rate calculation. The shale gas well simulated reservoir fracture fracturing liquid flowback rate experimental measurement device takes into comprehensive consideration the influence of stratum temperature, stratum stress and fluid pressure on shale flowback rate and quantifies influence factors to reflect the shale flowback rate under real stratum conditions and accordingly to providea theoretical basis for shale flowback scheme optimization.
Owner:SOUTHWEST PETROLEUM UNIV
Testing device for simulating equipment corrosion of continuous distillation industrial device
ActiveCN102445411AAvoid revaporizationEnable accurate simulationWeather/light/corrosion resistanceBoiling pointControl system
The invention relates to a testing device for simulating the equipment corrosion of a continuous distillation industrial device. The testing device comprises a continuous distillation system, a pressure control system and a corrosion sample system, wherein the top interface of a multi-mouth flask is connected with the bottom of a main reactor; the top interface of the main reactor is connected with the bottom of a main condenser; the top of the main condenser is connected with a non-condensable gas receiving tank, a cold trap, a gas buffering tank and an exhaust system through connecting pipes respectively, and is connected to a vacuum pump through the cold trap; and the corrosion sample system comprises a sample hanging device for placing samples into a steam phase and a liquid phase. A skillful reflow design is adopted in the invention, so that a condensed low-boiling-point liquid does not flow through the high-temperature main reactor, so that re-vaporization before reflowing to the bottom of the multi-mouth flask is avoided. Under the action of a reflow bypass, condensed low-boiling-point components and high-boiling-point components left at the bottom of the multi-mouth flask are fully mixed forcedly, and the distillation process is started once again, so that accurate simulation of continuous distillation is realized.
Owner:CHINA PETROLEUM & CHEM CORP +1
Determination method for content of tofacitinib citrate and related substances of tofacitinib citrate by reversed phase high-performance liquid chromatography
The invention discloses a determination method for the content of tofacitinib citrate and related substances of the tofacitinib citrate by reversed phase high-performance liquid chromatography. The determination method is characterized in that octadecylsilane chemically bonded silica is taken as filler; 0.02mol / L potassium dihydrogen phosphate solution and acetonitrile with volume ratio of 83:1 are taken as a mobile phase to perform isocratic elution, and the flow speed is 1.0ml / min; the 0.02mol / L potassium dihydrogen phosphate solution contains trimethylamine with mass concentration of 0.2 percent, and pH value is adjusted to 5.2 by using phosphoric acid; the detection wavelength is 290nm, and the column temperature is 30DEG C. According to the determination method disclosed by the invention, by adopting the isocratic elution, requirements on an instrument are reduced, as well as stable pressure and accurate and reliable experimental result are obtained; meanwhile, by adopting the method disclosed by the invention, the concentration of linear range is improved, and the preparation for a test solution during experiment is facilitated.
Owner:NINGBO LIWAH PHARM CO LTD
Comprehensive experiment method and experiment device for vibration stress analysis of HDD (horizontal directional drilling) rod
The invention provides a comprehensive experiment method for vibration stress analysis of an HDD (horizontal directional drilling) rod. According to the method, firstly, an experimental environment is established, the state is initialized, the rotating speed, the axial pull force and the torque are adjusted, hole wall limitation is simulated, and the vibration stress state of the drill rod in a hole under the condition of different rotating speeds, pull force, torque and drilling tool combinations is tested in real time. The invention also provides a comprehensive experiment device for vibration stress analysis of the HDD rod. The device comprises a drilling rod arranged horizontally and a drilling tool rotation system and further comprises a pull force torque control system, a stress monitoring system, a vibration displacement detecting system and an image acquisition system. The vibration stress of the drilling rod can be monitored and analyzed under condition of the different rotating speeds, axial pull force, torque and drilling tool combinations, an experiment system has a wide application range, displacement, stress and spatial form can be acquired simultaneously, and an experiment result is more real and reliable.
Owner:新疆鼎立非开挖工程有限公司
Small size simulation experiment table for underground coal mine exogenous fire
ActiveCN105894936AExperimental results are reliableMonitor the combustionEducational modelsFiberCollection system
The invention discloses a small size simulation experiment table for underground coal mine exogenous fire. The small size simulation experiment table comprises a tunnel and an auxiliary measurement and control system, wherein the auxiliary measurement and control system comprises a flue gas temperature collection system, a heat loss measurement system, a flue gas measurement alarm system, a mechanical ventilation and flue gas exhaust system and a flue gas flow field display system; the flue gas temperature collection system comprises a distributed fiber temperature measurement circuit mounted in the tunnel and a thermal infrared imager mounted at the opening of one end of a simulated tunnel; the heat loss measurement system comprises a heat flux sensor mounted in the tunnel; the flue gas measurement alarm system comprises a flue gas analyser measurement point and an ion flue gas sensing detection alarm which are mounted in the tunnel. The small size simulation experiment table can simulate the situation, flue gas components, flue gas flow rule, temperature field variation rule, poisonous and pernicious gas concentration variation rule and heat loss during fuel combustion in case of fire in a laboratory, and develops a suitable coal mine tunnel detection and control system.
Owner:XIAN UNIV OF SCI & TECH
High-pressure vapor explosion test device and system
InactiveCN105527316AHigh degree of simulationHigh simulationMaterial exposibilityTest analysisEngineering
The invention discloses a high-pressure vapor explosion test device and system. The test device comprises a pressure-bearing container, a pressure relief device, a heating device for heating the test medium in the pressure-bearing container, and a sensor, wherein the pressure-bearing container is provided with a cavity consistent with a high-pressure vapor medium injection pipe in inner diameter and is used for providing a load and test place for a high-pressure vapor medium, the side face of the bottom of the pressure-bearing container is provided with a medium injection opening for injecting the test medium into the cavity of the pressure-bearing container, the pressure relief device is of a hollow structure, is arranged at the top of the pressure-bearing container and is connected and communicated with the pressure-bearing container, a rupture disk is arranged in a hollow cavity of the pressure relief device, the sensor is arranged in the cavity of the pressure-bearing container and is used for collecting physical parameters of the test medium in the cavity of the pressure-bearing container. The high-pressure vapor medium injection pipe can be better simulated, the simulation degree of the high-pressure vapor explosion test device is improved, and more reliable technical parameter support is provided for high-pressure vapor explosion test analysis.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Preparation method for electron back scattered diffraction test sample of zirconium oxide thermal barrier coating
InactiveCN104089801ANo pollution in the processNo residual shear stressPreparing sample for investigationPollutionElectron
The invention relates to a preparation method for an electron back scattered diffraction test sample of a zirconium oxide thermal barrier coating. The preparation method comprises the steps of a. performing surface treatment on the electron back scattered diffraction test sample of the zirconium oxide thermal barrier coating; b. fixing the electron back scattered diffraction test sample of the zirconium oxide thermal barrier coating on a sample table of an argon ion beam grinder, and adjusting the center of the electron back scattered diffraction test sample of the zirconium oxide thermal barrier coating; c. vacuumizing to form a pressure being 1*10<-5>-1*10<-3>Pa, adjusting an inclination angle of the sample table for three times, wherein the inclination angle of the sample table is adjusted to be 60-85 degrees each time, and performing argon ion beam grinding on the electron back scattered diffraction test sample of the zirconium oxide thermal barrier coating; and d. resetting the inclination angle of the electron back scattered diffraction test sample of the zirconium oxide thermal barrier coating to zero degree, and performing spray-plating on the electron back scattered diffraction test sample of the zirconium oxide thermal barrier coating. The preparation method has the characteristics of no shear stress residue, no grinding material pollution, no scratches, low injury to the test sample and the like; a kikuchi band is relatively high in definition; the calibration rate of an electronic diffraction pattern is over 95 percent.
Owner:SHANGHAI INST OF MEASUREMENT & TESTING TECH +1
Top air-injection gravity auxiliary driving oil-extraction two-dimensional physical simulation experiment system
ActiveCN102654046AEnsure consistencyExperimental results are reliableSurveyFluid removalPilot systemGas phase
The invention discloses a top air-injection gravity auxiliary driving oil-extraction two-dimensional physical simulation experiment system, comprising a sample injection system, a simulation system, an output system, a PC (personal computer) monitoring system and a gas chromatograph-mass spectrometer, wherein the sample injection system comprises an ISO (international standard organization) high-precision calculation pump, a dead oil piston container, a natural gas piston container and a sample preparer; the simulation system comprises the ISO high-precision calculation pump, a formation water piston container, an air piston container, a three-way valve, a high-pressure bin, an inclined angle marking device and a two-dimensional sand filling model; the output system comprises a separator, a helium tank and a gas meter; and the PC monitoring system comprises a PC monitoring computer and a gas flow quality controller. Through an experiment system disclosed by the invention, the consistency of a sample and in-place oil can be ensured so that an experiment result is more real and reliable, and an online monitoring function for samples of an output system is exerted, and the real-time and automation degrees are improved.
Owner:PETROCHINA CO LTD
Nano island film with enhanced Raman signal and method for preparing nano island film
InactiveCN102706852ALow costIncrease manufacturing costMaterial nanotechnologyRaman scatteringPolystyreneSilicon dioxide
The invention discloses a nano island film with an enhanced Raman signal. The nano island film comprises a polystyrene film and silicon dioxide layers which are laminated together, wherein metal nano material layers wrap the surfaces of silicon dioxide particles, which are protruded on the polystyrene film. A method for preparing the nano island film comprises the following steps of: firstly, tightly arranging silicon dioxide particle layers on the surface of slide glass by using a vertical deposition method; secondly, injecting polystyrene into the silicon dioxide layers, drying, and aminating the surface, namely connecting amino to the surface of silicon dioxide, which is protruded on the polystyrene; thirdly, absorbing gold nano particles to the surface of the silicon dioxide; and finally, adding growth liquid and a hydrogen dioxide solution, stirring and growing, and thus obtaining the nano island film with the enhanced Raman signal. The island film is low in preparation cost and high in uniformity. The island film is a good Raman enhanced substrate.
Owner:SOUTHEAST UNIV
Reaction mechanism research and analysis method for amphoteric water-soluble catalyst aerobic oxidation of benzyl alcohol to benzaldehyde
ActiveCN108256286ADoes not affect the alcohol oxidation processShorten the development cycleChemical processes analysis/designSpecial data processing applicationsRate-determining stepBenzaldehyde
The invention discloses a reaction mechanism research and analysis method for amphoteric water-soluble catalyst aerobic oxidation of benzyl alcohol to benzaldehyde. A density functional theory (DFT) is used for research of reaction mechanism of an amphoteric water-soluble CuII / TEMPO catalyst system in catalysis and oxidization of alcohol into aldehyde in an alkaline aqueous solution. The calculation results show that zwitterionic properties of catalysts have no effect on oxidation reaction of the alcohol; the catalytic reaction mechanism includes catalyst activation, substrate oxidation and catalyst regeneration steps; the conversion efficiency (TOF=3.89h-1) is calculated based on an energetic span model and consistent with an experimental measurement result (TOF=5.40h-1). The rate-determining step is the substrate oxidation step, and is a process in which hydrogen atoms are transferred from alkoxide to oxygen atoms of TEMPO. By exploring the reaction mechanism of the catalytic system,the reaction process can be observed, and a theoretical basis is provided for designing a new high-efficiency catalyst.
Owner:INNER MONGOLIA UNIV OF TECH
Tire hydroplaning performance dynamic test experiment table and experiment method
ActiveCN105092266ACompact structureExperimental results are reliableVehicle tyre testingRoad surfaceContact position
The invention provides a tire hydroplaning performance dynamic test experiment table and experiment method, and belongs to the field of tire mechanical property test. The experiment table comprises a base table which is provided with a combined framework, a tire contact disc, a camera and data and image acquisition equipment. The combined framework is provided with a motor and a pressurization water container. The pressurization water container is provided with a water film ejector. The tire contact disc is arranged below a tested tire. The camera is arranged exactly below the contact position of the tested tire and the tire contact disc. The data and image acquisition equipment and the camera are connected via a data line. The experiment method comprises performance tests of the tested tire under the normal state and the side inclination and side deflection states and the state of combination of side inclination and side deflection. Various working conditions of the tire in high-speed operation on dry and wet pavements under the vertical or side inclination state can be simulated, and the tire hydroplaning performance dynamic test experiment table and experiment method are used for testing the mechanical property of the tire so that improved experiment data can be provided for research and development and performance analysis of the tire.
Owner:SHANDONG JIANZHU UNIV
Water-based heat-fluid-solid coupling true triaxial shear seepage test apparatus for coal body and test method using apparatus
ActiveCN105092449ASimple structureEasy to adjustPermeability/surface area analysisWater basedHeat flow
The invention discloses a water-based heat-fluid-solid coupling true triaxial shear seepage test apparatus for coal body and a test method using the apparatus. According to the test apparatus, on the basis of the prior art, a true triaxial test box with a separated structure is employed, and two rubber pallets each possessing a special structure matched with the test box are employed, so that the simulation authenticity, accuracy and reliability are substantially improved during shear deformation operation of a test piece, and monitoring is facilitated. According to the test apparatus, because the to-be tested test piece is subjected to the force along the three axial directions of a stereo coordinate system, the test apparatus is a true triaxial shear seepage test apparatus in a real sense. Compared with the prior art, the test method using the test apparatus is high in simulation degree, and possesses the characteristics of true and accurate result, good reliability and the like.
Owner:SHANDONG UNIV OF SCI & TECH
Method for detecting content of free sodium hyaluronate in medical cross-linking sodium hyaluronate gel
InactiveCN106053369AThe experimental results are accurateExperimental results are reliableColor/spectral properties measurementsWater bathsFiltration membrane
The invention relates to a method for detecting the content of free sodium hyaluronate in medical cross-linking sodium hyaluronate gel. The detection method comprises the following steps: weighing a certain number of samples, adding an appropriate quantity of a PBS solution, enabling the weighed samples to be dissolved in the PBS solution so as to obtain a solution, uniformly mixing the solution, filtering the mixed solution with a filtration membrane, rinsing a vials with an appropriate quantity of PBS, taking a filtrate, and determining the content of glucuronic acid; (2) preparing a series of glucuronic acid standard fluid; (3) separately adding 5 mL of a 0.025 mol / L sodium tetraborate sulfuric acid solution to a tube loaded with the series of the standard fluid and a sample tube, performing uniform mixing, performing heating in boiling water bath for 10 minutes, performing cooling to room temperature, adding 0.2 ml of a carbazole ethanol solution to the tubes, performing uniform mixing, performing heating in boiling water bath for 15 minutes, performing cooling to room temperature, and determining the absorbance of the standard tube and the sample tube at the position of 530 nm with a spectrophotometer; and (4) checking the content of the glucuronic acid in the sample tube according to the absorbance of the sample tube from a standard curve, and then calculating the content of the free sodium hyaluronate according to a calculation formula. The method disclosed by the invention is used for detecting the content of the free sodium hyaluronate, the experimental results are stable and reliable, and the repeatability is good.
Owner:ZHEJIANG JINGJIA MEDICAL TECH CO LTD
Floating sphere immunofluorescent staining method and staining device
The invention discloses a floating sphere immunofluorescent staining method and a staining device, belonging to methods for coloring a sample for test. The staining method comprises the following steps: separation of spheres from a culture medium, fixing by stationary liquid, adding of Triton X-100 transparent cells, closing by confining liquid, adding of an interest protein-resisting primary antibody working solution, adding of a primary antibody-resisting fluorescent secondary antibody working solution, and cell nucleus staining by DAPI dye liquor. The staining device comprises a cell chamber and a staining sleeve part arranged at the outer part of the lower end of the cell chamber in a sleeving way. The floating sphere immunofluorescent staining method has the advantages of high efficiency, simpleness, convenience and the like, an antibody usage amount is reduced, experiment cost is reduced, manpower and time are saved, an accurate and reliable experiment result is obtained on the basis that a fundamental principle that immunofluorescent staining is not changed and specially training experimenters is not needed, sphere loss in an experiment operation process is avoided, an experiment success rate is increased, and the efficiency is improved.
Owner:GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
Microfluidic chip applicable to medicine screening of zebra fish embryos
InactiveCN102757885ASave human effortSimple and cheap to manufactureBioreactor/fermenter combinationsBiological substance pretreatmentsElastomerControl layer
The invention relates to a microfluidic chip applicable to medicine screening of zebra fish embryos. The microfluidic chip is low in price, easy to prepare and suitable for large scale, high flux and automation. The microfluidic chip comprises at least one microfluidic layer, at least one control layer, a connecting layer and at least one micro cell, wherein the control layer is adjacent to the microfluidic layer; the connecting layer is used for connecting the adjacent microfluidic layer and the control layer; the micro cell is used for accommodating the zebra fish embryos and providing the growth environment for the zebra fish embryos; the microfluidic layer is provided with a microfluidic channel; the microfluidic channel is communicated with the internal space of the micro cell; the control layer is provided with at least one control channel; the microfluidic channel and the control channel are provided with at least one intersected point; and the connecting layer at the position of the at least one intersected point consists of an elastomer material and forms an elastomer valve. The communication state of the microfluidic channel can be controlled convenient through the elastomer valve, so that fully-automatic operation of medicine screening of the zebra fish embryos can be realized through an electromagnetic valve controlled by an external computer.
Owner:PEKING UNIV
Comprehensive shear tester for anchoring bodies and test method thereof
PendingCN109115632AAvoid affecting the experimental resultsSo as not to damageMaterial strength using steady shearing forcesEngineering
Owner:HENAN POLYTECHNIC UNIV
Photoelectrochemistry detection device with double detection pools and use method of photoelectrochemistry detection device
InactiveCN105203527AReduce or even eliminate photocurrent fluctuationsReduce or even eliminate limit driftAnalysis by subjecting material to chemical reactionSignal-to-noise ratio (imaging)Instability
The invention relates to a photoelectrochemistry detection device with double detection pools and a use method of the photoelectrochemistry detection device. The device comprises a sample pool, a reference pool, a light source, a dark box and a voltage measurement recording device, wherein one side surface of the dark box is provided with a light valve assembly; the sample pool and the reference pool are arranged in the dark box; the light source is arranged at one side of the light valve assembly; a first light-sensitive electrode, a first pair of electrodes and a solution to be tested are arranged in the sample pool; a second light-sensitive electrode, a second pair of electrodes and a blank solution are arranged in the reference pool; the voltage measuring recording device respectively measures and records the voltage between the first light-sensitive electrode and the first pair of electrodes and the voltage between the second light-sensitive electrode and the second pair of electrodes. By using the double-passage detection pools, on the basis of not changing the existing operation conditions, the detection signal fluctuation due to factors such as light source instability and temperature change in the detection pools in the detection process can be counteracted through reference. The signal-to-noise ratio of the detection system is improved; the trace ingredient determining sensitivity of an electrochemiluminescence method is improved.
Owner:SHANDONG NORMAL UNIV
Acidic etching instrument and operation method thereof
InactiveCN103234971AAvoid errorsAvoid conditionsMaterial analysis by optical meansExperimental researchRock core
The invention discloses an acidic etching instrument and an operation method thereof. The acidic etching instrument comprises a visualized core holder made of transparent materials, wherein the visualized core holder is of a hollow sealed structure, the upper end of the visualized core holder is provided with an upper through hole, the lower end of the visualized core holder is provided with a lower through hole, and the upper through hole and the lower through hole are both communicated with the inside of the visualized core holder. The acidic etching instrument and the operation method thereof disclosed by the invention have the beneficial effects that the instrument adopts a one-inch columnar core, therefore, an effect of easy processing is achieved, and actual reservoir cores can be adopted for carrying out experimental researches; the etching form of cores can be subjected to real-time tracking; and the whole acidic rock reaction process is reflected really, and a study on the change of annular fracture diverting capacity can be performed, thereby achieving the purpose of optimizing an acidizing fluid system and an acidizing process.
Owner:SOUTHWEST PETROLEUM UNIV
Infiltration and suction system for enhancing carbonated water under high temperature and high pressure conditions
ActiveCN107727554ASpeed up the imbibition processShorten the cycle of imbibitionPermeability/surface area analysisLine tubingRock core
The invention relates to an infiltration and suction system for enhancing carbonated water under high temperature and high pressure conditions. The infiltration and suction system comprises a carbonated water preparation system, a carbonated water injection system and a rock core infiltration and suction system; the carbonated water preparation system is connected with the rock core infiltration and suction system through a pipeline; the carbonated water injection system injects the carbonated water into the rock core infiltration and suction system; the carbonated water in the rock core infiltration and suction system can rotationally flow; and the carbonated water is an aqueous solution in which CO2 is dissolved. An electromotor is mounted in the infiltration and suction system, can drive a rotating plate and a rotating rod in a metal container to rotate and further drives a fluid to rotate, so that on one hand, the end surface at the lower par of a rock core cannot be shielded, andon the other hand, the infiltration and suction process of the rock core is accelerated, and therefore, the infiltration and suction period of the rock core is greatly shortened, and the infiltrationand suction efficiency is improved; and the fluid rotation and the rotating speed setting can aim at different types of rock cores and different types of crude oil, so that the phenomenon that oil droplets are adsorbed on the surface of the rock core is effectively eliminated.
Owner:CHINA UNIV OF PETROLEUM (BEIJING)
Three-dimensional tumor model decellularization porous scaffold, construction method and application thereof
ActiveCN107988158AImprove adhesionPromote growthCell culture supports/coating3D cultureCell-Extracellular MatrixCell adhesion
The invention belongs to the fields of cell biology and tumor tissue engineering materials and provides a three-dimensional tumor model decellularization porous scaffold, a construction method and application thereof. The whole pig lung scaffold is prefrozen, a uniform part without obvious bronchi is selected and sliced, purified water and PBS are added for cleaning until no blood streak is formed, removing cells with sodium dodecyl sulfate and TritonX-100, and freezing, drying and chemical crosslinking methods are adopted, so that a crosslinked decellularization pig lung three-dimensional tumor model scaffold is obtained. The three-dimensional tumor model decellularization porous scaffold provided by the invention has the advantages that decellularization matrix sourced from pig lung tissues is taken as a scaffold material for constructing a tumor model, on the basis of effectively removing cell components, a pulmonary alveolus-bronchus structural vein network is reserved as soon as possible, a natural extracellular matrix microenvironment can be simulated, and cell adhesion, growth and proliferation are facilitated. The in vitro constructed three-dimensional tumor model after cells are inoculated has a tissue structure closer to an in vivo tissue and is applicable to screening study of anticancer drugs.
Owner:DALIAN UNIV OF TECH
Detection method of folate metabolism related gene
InactiveCN107254520AReduced activityLow costMicrobiological testing/measurementGel electrophoresisRelated gene
The invention discloses a detection method of a folate metabolism related gene, which includes steps of extracting DNA from oral mucosa cell of a detector; amplifying MTHFR gene C677T by SEQ NO 1 and SEQ NO 2; amplifying MTHFR gene A1298C by SEQ NO 3 and SEQ NO 4; amplifying MTHFR gene A66G by SEQ NO 5 and SEQ NO 6; extracting DNA from oral mucosa cell as a template to perform PCR reaction; performing sepharose gel electrophoresis of 1% of ordinary PCR amplified product on three pairs of primers; recycling a strip of a kit recycling item from the 1% of agarose gel electrophoretic band by sepharose gel; performing sequence testing reaction; purifying the sequence testing reaction product and denaturing; testing sequence by an upper 3730 sequence tester; analyzing three SNP gene models of the sequence testing result by Chromas software. The method has the advantages of high detection accuracy, and convenience of use.
Owner:安徽安龙基因科技有限公司
Experimental system and method for turbine blade leading edge cooling test under rotating condition
ActiveCN111735844AExperimental results are reliableFacilitate comparison of effects on heat transfer characteristicsMachine part testingMaterial heat developmentTarget surfaceTurbine blade
The invention discloses an experimental system and method for turbine blade leading edge cooling test under a rotating condition. A whole blade leading edge cooling system is imitated, a real airflowimpact boundary condition is provided, and the comprehensive influence of the mixing of heating incoming flow at external relative speed and internal cooling air flow, the arrangement mode of air flowimpact channels, the arrangement of shower head air film cooling holes at the front edge target surface and the rotation of different areas (blade root, blade body and blade top) of the blade is considered. In the experiment process, temperature, pressure and flow signals are monitored, controlled and adjusted, so that the interior-air film coupling cooling performance of the rotary turbine bladeis accurately evaluated, and reliable reference is provided for design and application of an actual blade cooling structure.
Owner:XI AN JIAOTONG UNIV
An engine low-pressure simulation testing platform
InactiveCN103364197ASolve the problem that the engine test under the low pressure state cannot be simulatedNo need to spend moneyInternal-combustion engine testingDynamometerAtmospheric pressure
The invention discloses an engine low-pressure simulation testing platform. The engine low-pressure simulation testing platform comprises an airtight case body. A temperature adjusting device, an air pressure adjusting device, and an engine cooling device are disposed on the case body. A dynamometer, a dynamometer cooling device, and an engine oil supplying device are disposed in the case body. The engine low-pressure simulation testing platform is capable of simulating a low-atmospheric-pressure environment of a plateau at a low-altitude area and provides convenience for engine test.
Owner:GUANGXI YUCHAI MASCH CO LTD
Closed integrated pathogen detection card box
PendingCN111235008AAvoid direct contactAvoid cross-contaminationBioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyLiquid storage tank
The invention relates to a closed integrated pathogen detection card box. The closed integrated pathogen detection card box mainly comprises a liquid storage base, a liquid suction and discharge assembly and a sealing cover, wherein the liquid storage base can be fixedly arranged in an external metal hot trap device and rotates along with the rotation of the external metal hot trap device, so a liquid suction head in the liquid suction and discharge assembly can enter each liquid storage tank. The whole pathogen detection card box mainly has the following advantages: a piston rod and the liquid suction head in the liquid suction and discharge assembly is externally controlled to move up and down and cooperates with rotation of the liquid storage base, so infectious pathogen integrated detection can be integrally and automatically completed in a closed environment at a time; operation is convenient; the level requirement of experimenters can be effectively reduced; cross contamination can be avoided; and the closed integrated pathogen detection card box is applicable to a POCT system.
Owner:深圳市朗司医疗科技有限公司
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