Closed integrated pathogen detection card box

Abstract

The invention relates to a closed integrated pathogen detection card box. The closed integrated pathogen detection card box mainly comprises a liquid storage base, a liquid suction and discharge assembly and a sealing cover, wherein the liquid storage base can be fixedly arranged in an external metal hot trap device and rotates along with the rotation of the external metal hot trap device, so a liquid suction head in the liquid suction and discharge assembly can enter each liquid storage tank. The whole pathogen detection card box mainly has the following advantages: a piston rod and the liquid suction head in the liquid suction and discharge assembly is externally controlled to move up and down and cooperates with rotation of the liquid storage base, so infectious pathogen integrated detection can be integrally and automatically completed in a closed environment at a time; operation is convenient; the level requirement of experimenters can be effectively reduced; cross contamination can be avoided; and the closed integrated pathogen detection card box is applicable to a POCT system.

Description

technical field [0001] The invention relates to a closed integrated pathogen detection cartridge, which belongs to the fields of molecular diagnosis and biomedicine. Background technique [0002] Nucleic acid amplification detection has been widely used in biomedicine and other fields. In the traditional process of nucleic acid amplification detection, it is common for false positive detection results to be caused by cross-contamination of amplified products. Moreover, most of the templates used in traditional nucleic acid amplification detection methods need to go through an independent sample extraction step, and this step is also likely to cause template cross-contamination and affect the judgment of sample test results. [0003] The on-site detection of pathogen nucleic acid has good application prospects for epidemic prevention and control and epidemiological investigation, and is also very suitable for biological anti-terrorism, agriculture and food safety. Therefore,...

AI Technical Summary

Problems solved by technology

However, these detection cards or detection tubes can only detect one nucleic acid at a time, so a large number of instruments need to be carried, which brings inconvenience to on-site operations

At present, there are some integrated nucleic acid detection devices on the market, such as GeneXpert System, etc., which can realize the extraction, amplification and detection of the nucleic acid to be tested in a closed system, which greatly avoids the contamination of samples during the extraction and amplification process , effectively improving the accuracy and operability of nucleic acid detection, but these detection devices generally have the problems of large size, complex structure, and high detection cost, and these detection devices are all developed and produced abroad, and the equipment is expensive; Some miniaturization uses visual nucleic acid detection devices such as test strip colloidal gold, but there is a problem of low detection accuracy during the detection process

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