Temperature control method and device for polymerase chain reaction biochip

Abstract

The invention provides a temperature control method and a device for a polymerase chain reaction biochip. The temperature control device for the polymerase chain reaction biochip comprises a power module, a temperature collecting module, a signal amplifying module, a data collecting card module, a chip heating module and a chip cooling module. The temperature collecting module converts a resistance signal of a platinum temperature sensor on the biochip into a voltage signal through the stimulating of a power supply. The signal is inserted into a Lab VIEW signal colleting card after amplification and noise filtering. The collecting card possesses an A / D conversion function and a signal conditioning function, and then the digital voltage signal enters a LABVIEW temperature control program. An analog control signal is output through the data collecting card, the temperature of the chip is enabled to meet a reaction requirement by starting a chip heating or cooling module. A temperature control curve is displayed on a PC (personal computer) display screen in real time in a reaction process. The device provided is capable of achieving loosed-loop automatic temperature control, good in heating and cooling processes, good in stability and high in human-machine interactivity. The temperature control method and the device for a polymerase chain reaction biochip are a novel detection analysis method and novel detection analysis equipment.

Description

technical field [0001] The invention relates to a biological chip temperature control device, in particular to a PCR biological chip temperature control method and device based on LabVIEW. Background technique [0002] Polymerase chain reaction (PCR), which emerged in the 1980s, is a primer-mediated enzymatic synthesis of DNA sequences in vitro. It is a three-step reaction at different temperatures consisting of temperature control and enzyme-catalyzed processes. A process that repeats periodically. Due to its unprecedented sensitivity and specificity, it has become a key technology in gene research and is widely used in various fields of molecular biology. The key to the PCR reaction is to repeat and control the cycle temperature, while the traditional PCR amplification instrument has a slow heating and cooling speed and consumes a large amount of expensive biochemical reagents, so the work efficiency is low. [0003] Virtual instrument (LABVIEW) is the basis of modern la...

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Problems solved by technology

However, traditional instruments, such as the PCR amplification instrument here, are closed to researchers, not open and scalable, and not conducive to innovative research

[0004] After searching, it was found that in the invention patent application with the Chinese patent application number 01103687.7, a micro-temperature control device based on a single-chip microcomputer was proposed, which can realize temperature control automation, but the program of the system is fixed, the function is simple, and it cannot display real-time temperature. And the chip adopts air cooling, the cooling rate is slow

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