The invention discloses an auriculiformis
open type tissue culture method. The method comprises the following steps: using an annual
branch with plump axillary buds from April to October as an explant, disinfecting, and culturing in an
open type shoot induction medium to obtain the axillary buds, performing
open type test-tube rooting culture on the axillary buds in an open type rooting medium to obtain rooting seedlings;
cutting the rooting seedlings into sections each having at least one
bud as successive explants, and continuously culturing in the open type rooting medium for rooting for many generations to realize the aim of multiplication. A
bacteriostatic agent,
chlorothalonil, used by the method is simple in component, low in cost and easy to buy, and a good
antibacterial effect can be realized by just using 0.2.L<-1>; by
screening study on the explant type, the disinfection method, the explant collection season, the
shoot induction medium and the like, the
shoot inductivity is up to 99.54%; the rooting percentage is 97.62%, the multiplication multiple of rooting for many generations can reach 3.58, so as to completely meet the requirements of industrialized production.