33 results about "Screening study" patented technology

The invention discloses a chromatographic column decorated by lipid rafts. The chromatographic column is a silica gel chromatographic column which is decorated with the lipid rafts on the surface. A TrkA lipid raft-sillica gel stationary phase chromatographic system is built firstly. Stationary phase can be combined with medicinal specificity of tyrosine kinase receptors as a target spot, and then reservation is produced, but non-reservation behavior is not existed in the TrkA lipid raft-sillica gel chromatographic column of the tyrosine kinase target spot medicines. Lipid raft-sillica gel chromatograph which is rich in TrkA receptors is a novel medicine screening model which has a living being affiliation character and is capable of selective recognition medicines, wherein the medicines are reacted with the TrkA receptors. The chromatographic system is different from the traditional chromatographic system and has double characters of chromatograph separation and activity recognition. Therefore, the chromatographic system can be used for guide medicine screening study where the TrkA receptors served as drones. The chromatographic column decorated by the lipid rafts is used through different time and different moving phases, and the TrkA lipid raft-sillica gel stationary phase chromatographic system is proved stable. The chromatographic column is good in repeatability, long in service life and has good application prospects. The invention discloses a manufacturing method of the chromatographic column decorated by the lipid rafts.

The invention relates to building of an in-vitro three-dimensional cell model of a breast cancer and an application of the in-vitro three-dimensional cell model in research of a drug resistance mechanism and reversal agents screening. A certain amount of human breast cancer MCF-7 cells are planted on a Millicell cell culture chamber cell by applying a'3D no base and embedded' method; and a three-dimensional multi-layer cell model of a human breast cancer MCF-7 cell, of which the thickness is about 40 microns, is formed by cultivation and the number of layers is 10-12. Transport kinetics and consuming behaviors of chemotherapy adriacin doxorubicin are investigated on the MCF-7 multi-layer cell model by single use and combination of P-GP inhibitors LY335979 to prove the drug resistance in the culture process; and the in-vitro three-dimensional cell model is applied to screening of an adriamycin resistance-reversal agent ginsenoside 20(S)-Rh2.

The invention provides a specific primer, a kit and an identification method for identifying traditional Chinese medicine kwangsi gecko. A lot of primers are designed based on a CYTB gene of the kwangsi gecko; the primer with species specificity is screened out through the screening study of a large amount of primers; the primer is used for identifying the traditional Chinese medicine kwangsi gecko, so that the kwangsi gecko medicinal material can be accurately separated from other adulterants, a PCR (Polymerase Chain Reaction) amplification product is only 100 to 250bp, the molecular weight is low, a lot of traditional Chinese medicine kwangsi gecko is ensured to be identified within a short time, and an identification result is accurate, stable, reliable and high in sensitivity.

Provided is a screening method for low-nitrogen-resistant barley. Overground parts of the barley are used as screening research objects, a hydroponic method is used for selecting the shoot dry weightof the barley in the seedling stage as a screening index, low-nitrogen-resistant barley varieties are screened out, the appropriate low nitrogen stress concentration is determined, and the product ofrelative single plant nitrogen uptake and relative nitrogen physiological utilization efficiency is used for judging the tolerance degree of the barley to the low nitrogen environment to some extent to reflect the low nitrogen tolerance of barley materials. The low nitrogen tolerance of the barley is characterized by the shoot dry weight and the product of the relative single plant nitrogen uptakeand the relative nitrogen physiological utilization efficiency, which is more accurate compared with the characterization of low nitrogen tolerance by one aspect of nitrogen uptake or nitrogen physiological utilization efficiency. According to the barley screened by the method, the application rate of nitrogen fertilizers is effectively reduced, the environmental burden is reduced, and the application rate of the nitrogen fertilizers can be reduced to at least about one third of the total nitrogen to greatly alleviate the environmental pressure.

The invention discloses a group of substituted benzoheterocycle amine derivatives and a preparation method and related application thereof as an IMPDH (inosine monophosphate dehydrogenase) inhibitor. The IMPDH inhibitor has good application prospects in virus resistance, immunosuppression, tumor resistance, bacterium resistance, parasite resistance and the like. In the invention, a new-structure IMPDH inhibitor shown by the formula (I) is obtained through the design, synthesis and activity screening study on an active compound targeting IMPDH, and a foundation is laid for the development and application of the compounds as the medicines and medicinal compositions thereof with related effects such as virus resistance, tumor resistance, immunosuppression and the like.

The invention discloses a method for screening high-efficiency varieties of double-cropping super rice with water and fertilizer synergy, which includes screening seeds, treating nitrogen application rate as the main area, and split-plot irrigation method for split-plot test, calculating average yield, data processing, and variety screening And other steps can quickly, conveniently, efficiently, and low-cost screen out varieties with high water-fertilizer synergy in double-cropping super rice, which solves the problems of single screening elements and unscientific screening in existing screening research.

The invention discloses a chromatographic column decorated by lipid rafts. The chromatographic column is a silica gel chromatographic column which is decorated with the lipid rafts on the surface. A TrkA lipid raft-sillica gel stationary phase chromatographic system is built firstly. Stationary phase can be combined with medicinal specificity of tyrosine kinase receptors as a target spot, and then reservation is produced, but non-reservation behavior is not existed in the TrkA lipid raft-sillica gel chromatographic column of the tyrosine kinase target spot medicines. Lipid raft-sillica gel chromatograph which is rich in TrkA receptors is a novel medicine screening model which has a living being affiliation character and is capable of selective recognition medicines, wherein the medicines are reacted with the TrkA receptors. The chromatographic system is different from the traditional chromatographic system and has double characters of chromatograph separation and activity recognition. Therefore, the chromatographic system can be used for guide medicine screening study where the TrkA receptors served as drones. The chromatographic column decorated by the lipid rafts is used through different time and different moving phases, and the TrkA lipid raft-sillica gel stationary phase chromatographic system is proved stable. The chromatographic column is good in repeatability, long in service life and has good application prospects. The invention discloses a manufacturing method of the chromatographic column decorated by the lipid rafts.

The invention relates to a new vascular tissue generation method. The method comprises, removing thoracic aorta, heart tissue, liver tissue, spleen tissue, lung tissue, kidney tissue and colon tissue from a mouse or a rat under a sterile condition; carrying out a combination of the removed tissues and the culture plate to generate the new vascular tissues respectively corresponding to the aorta, the heart tissue, the liver tissue, the spleen tissue, the lung tissue, the kidney tissue and the colon tissue. With the method provided by the present invention, disadvantages of cumbersome quantity and not high survival rate are overcome; a simple and convenient model of the new vascular tissue generating is established, wherein the model can be widely used; the method further can be applicable for screening studies of the tumor angiogenesis inhibitor.

The invention provides a specific primer, kit and identification method for identifying donkey-derived components. A large number of primers are designed based on the CYT B and COX-1 genes of donkeys, and a large number of primers are screened and studied. Specific primers, using the above primers to identify donkey-derived components in the test mixture, can accurately separate donkeys from other confounding products, and the PCR amplification product is only 50-150bp, with a small molecular weight, ensuring its A large number of donkey-derived components in the mixture are identified in a short period of time, and the identification results are accurate, stable, reliable, and highly sensitive.

The invention relates to a yageine derivative and salts of compounds. The invention synthesizes the novel yageine derivative compounds which can strengthen the antineoplastic activity and reduce the toxicity of a nervous system through the structural modification to locus 1, 2, 3, 7 and 9 of beta-carboline parent nuclei of yageine. The compounds can be used for preparing drugs used to treat various tumor diseases through the screening study of in vivo and in vitro anti-tumor experimental models.

The present invention provides a method for quantitative calculation of paleo-depth for lacustrine deposition, the method for quantitative calculation of paleo-depth for lacustrine deposition includes: step 1, screening lakes similar to the research area; step 2, for the screened lakes, establishing Quantitative relationship between lake bottom sediment TOC and water depth; step 3, conduct mudstone TOC test for the research area, and count the stratum thickness of the target layer; step 4, establish the quantitative relationship between ancient water depth data and stratum thickness; and step 5, draw ancient Plane distribution of water depth. The paleo-depth quantitative calculation method for lacustrine sediments further improves the means of paleo-depth restoration, and provides new research ideas and technical means for the study of paleo-lake sedimentary evolution process and the prediction of multi-type lacustrine sand bodies.

The invention provides a specific primer, a kit and an identification method for identifying traditional Chinese medicine kwangsi gecko. A lot of primers are designed based on a CYTB gene of the kwangsi gecko; the primer with species specificity is screened out through the screening study of a large amount of primers; the primer is used for identifying the traditional Chinese medicine kwangsi gecko, so that the kwangsi gecko medicinal material can be accurately separated from other adulterants, a PCR (Polymerase Chain Reaction) amplification product is only 100 to 250bp, the molecular weight is low, a lot of traditional Chinese medicine kwangsi gecko is ensured to be identified within a short time, and an identification result is accurate, stable, reliable and high in sensitivity.

The invention discloses a high-efficiency micromotor rotor magnetic ring, which is composed of ferrite magnetic powder, poly 1,9-nonylidene terephthalamide, polylaurolactam, nylon 6 / clay nanocomposite material, oleic acid Injection molding is obtained. The rotor magnetic ring of the high-efficiency micro-motor provided by the present invention obtains the best component raw materials and their optimal dosage ratio through a large number of experimental screening studies, and adopts the method provided by the present invention to prepare the rotor magnetic ring of the motor, which can not only reduce the The quality of the motor rotor, while reducing the weight of the whole machine, improves the efficiency of the motor, the motor has lower noise, higher operating frequency, and larger pull-out torque, which has made a major breakthrough in the quality of the motor and achieved significant technological progress.

The invention provides a kind of Gynecology Tiaojing Sustained Release Dropping Pill and its preparation method. By optimizing the extraction process of the original medicinal material and adopting the double extraction method of alcohol extraction and water extraction, the active components of the Chinese medicinal material can be extracted more fully. At the same time, in order to overcome the The defects of the prior art, through a large number of tests and screening studies, use ethyl cellulose compounded with carbomer with good biodegradability and good sustained release effect as the skeleton material, and then add an appropriate amount of polyethylene glycol to make a slow release agent. Release drop pill preparation, its product not only has stable quality, can reduce the number of times of taking medicine, but also can maintain blood drug concentration stable and enhance its drug effect.

The invention belongs to the technical field of drugs, and particularly relates to a anti-fungal-infection pharmaceutical composition and use thereof. An alkaloid compound of a formula (I) has the useof anti-fungal infections, and a new candidate drug is provided for the treatment of fungal infections. In addition, it is further found that the combined use of the compound of the formula (I) and itraconazole has a synergistic antifungal effect through a combined drug experiment, while the amount of active ingredients of the drug is reduced, the drug resistance of microorganisms and the human side effects are reduced, and a foundation is further laid for the screening research of novel antifungal drugs.

The invention discloses an auriculiformis open type tissue culture method. The method comprises the following steps: using an annual branch with plump axillary buds from April to October as an explant, disinfecting, and culturing in an open type shoot induction medium to obtain the axillary buds, performing open type test-tube rooting culture on the axillary buds in an open type rooting medium to obtain rooting seedlings; cutting the rooting seedlings into sections each having at least one bud as successive explants, and continuously culturing in the open type rooting medium for rooting for many generations to realize the aim of multiplication. A bacteriostatic agent, chlorothalonil, used by the method is simple in component, low in cost and easy to buy, and a good antibacterial effect can be realized by just using 0.2.L<-1>; by screening study on the explant type, the disinfection method, the explant collection season, the shoot induction medium and the like, the shoot inductivity is up to 99.54%; the rooting percentage is 97.62%, the multiplication multiple of rooting for many generations can reach 3.58, so as to completely meet the requirements of industrialized production.

The invention relates to the fields of biomedical engineering technology and regenerative medicine, in particular to a preparation method of tissue-engineered beating tissue. In the present invention, stem cells derived from brown fat are induced into sinoatrial node-like cells, which are planted on collagen sponge, and tissue-engineered sinoatrial node-like pulsating tissue is constructed in vitro. The invention obtains a tissue engineered tissue with pacing properties, provides a tissue model for screening arrhythmia drugs and other studies, and provides a tissue model for the treatment of sick sinus syndrome and arrhythmia disease caused by atrioventricular conduction block. Here comes hope.