Screening method for actinomycetes with effect of inhibiting anthrax pathogenic bacteria

A technology of inhibition and screening methods, applied in the field of microorganisms, to achieve the effects of simple operation, simple process and low labor intensity

Inactive Publication Date: 2019-02-19
NINGDE NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a screening method for ac

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A screening method for actinomycetes with inhibitory effect on anthrax pathogenic bacteria, comprising the following steps:

[0028] A: Sample collection: Randomly collect 3 soil samples from the land under the Camellia oleifera tree, pack and mark them and bring them back to the laboratory. The soil samples are soil at a depth of 5cm underground;

[0029] B: Preparation of soil suspension: Weigh 8g of soil and pour it into an Erlenmeyer flask equipped with 90ml of phosphate buffer. and dilute this tenfold to 10 -4 、10 -5 and 10 -6 suspension.

[0030] C: Culture: put 10 -4 、10 -5 and 10 -6 The soil suspension was coated on Gao's No. 1 medium, and each gradient was repeated 3 times, evenly coated, and placed upside down at 28°C for 5 days at a constant temperature to obtain cultured actinomycetes;

[0031] D: Preliminary screening: After activating the pathogenic bacteria from the PDA medium by using the plate confrontation method, use a 5mm hole punch to make th...

Embodiment 2

[0035] A screening method for actinomycetes with inhibitory effect on anthrax pathogenic bacteria, comprising the following steps:

[0036] A: Sample collection: Randomly collect 3 soil samples from the land under the Camellia oleifera tree, pack and mark them and bring them back to the laboratory. The soil samples are soil at a depth of 10cm underground;

[0037] B: Preparation of soil suspension: Weigh 9g of soil and pour it into an Erlenmeyer flask equipped with 90ml of phosphate buffer. and dilute this tenfold to 10 -4 、10 -5 and 10 -6 suspension.

[0038] C: Culture: put 10 -4 、10 -5 and 10 -6 The soil suspension was coated on Gao's No. 1 medium, and each gradient was repeated 4 times, evenly coated, and placed upside down at 28°C for 8 days at a constant temperature to obtain cultured actinomycetes;

[0039] D: Preliminary screening: After activating the pathogenic bacteria from the PDA medium using the plate confrontation method, use a 6mm hole punch to make the ta...

Embodiment 3

[0043] A screening method for actinomycetes with inhibitory effect on anthrax pathogenic bacteria, comprising the following steps:

[0044] A: Sample collection: Randomly collect 3 soil samples from the land under the Camellia oleifera tree, pack and mark them and bring them back to the laboratory. The soil samples are soil at a depth of 15cm underground;

[0045] B: Preparation of soil suspension: Weigh 10g of soil and pour it into an Erlenmeyer flask equipped with 90ml of phosphate buffer. and dilute this tenfold to 10 -4 、10 -5 and 10 -6 suspension.

[0046] C: Culture: put 10 -4 、10 -5 and 10 -6 The soil suspension was coated on Gao's No. 1 medium respectively, and each gradient was repeated 5 times, evenly coated, and placed upside down at 28°C for 10 days at a constant temperature to obtain cultured actinomycetes;

[0047] D: Preliminary screening: After activating the pathogenic bacteria from the PDA medium by using the plate confrontation method, use a 7mm hole ...

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PUM

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Abstract

The invention discloses a screening method for actinomycetes with an effect of inhibiting anthrax pathogenic bacteria, and relates to the technical field of microorganisms. The method comprises the following steps of A, sample collection, B, soil suspension preparation, C, culturing, D, preliminary screening and E, secondary screening, and finally the actinomycetes with the effect of inhibiting the anthrax pathogenic bacteria is screened out. The actinomycetes with the effect of inhibiting the anthrax pathogenic bacteria can be quickly screened out in the modes of preliminary screening and secondary screening, the rate of novel antimicrobial active bacterial strains and antimicrobial active substances can be increased, the whole screening process is quick, the operation is simple, and thelabor intensity is lower; the antimicrobial active bacteria and the actinomycetes can be quickly, visually and efficiently screened out, and great convenience is provided for screening study of the antimicrobial active bacteria and the actinomycetes.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a screening method for actinomycetes with inhibitory effect on anthrax pathogenic bacteria. Background technique [0002] Tea trees are native to China. They are perennial evergreen woody plants of the genus Camellia in the family Theaceae. They like shade and humidity, and they are mostly distributed in subtropical regions. Most areas in Fujian Province have lush vegetation, high mountains and many clouds, which are conducive to the growth and development of tea trees. Therefore, the tea trees in Fujian The tea planting area is expanding continuously. For a long time, Fujian's tea production ranks first in the country. Ningde City is the largest tea-producing prefecture-level city in Fujian Province, and its planting area and production account for about 1 / 3 of the province. [0003] According to relevant reports and statistics, there are more than 100 kinds of tea tree disease...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12Q1/04C12R1/04
CPCC12N1/02C12Q1/04
Inventor 刘伟彭成彬阮宜朴陈美霞阮俊峰吴箭生余晓玲汤钰雯江胜滔郑世仲
Owner NINGDE NORMAL UNIV
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