A screening method for low-nitrogen-tolerant barley
A screening method and barley technology, applied in horticultural methods, botanical equipment and methods, nitrogen fertilizers, etc., can solve the problems of inability to compare the differences of the tested varieties, and achieve the goal of alleviating environmental pressure, avoiding inaccuracies, and reducing environmental burdens. Effect
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[0032] 19 materials from the Shanghai area were selected as the test materials, see Table 1.
[0033] Table 1
[0034]
[0035] Take an appropriate amount of barley seeds, first sterilize them with 10% sanitin for 10 minutes, rinse them with clean water, soak the seeds at room temperature for 4-6 hours, then germinate them overnight, and put them on moist filter paper to germinate after the seeds are white.
[0036] Seven days later, take barley seedlings that grow consistently, wrap them with sponge strips, move them into perforated foam boards, and distribute 40 holes (5×8) on average on each foam board, and put them into a turnover tank filled with nitrogen nutrient solution. Box (the size of the turnover box is 42cm×30cm×10cm), pH 6.0, temperature 20°C, replenish water every day, manage diseases and insect pests well, and replace the nutrient solution once a week.
[0037] The low-nitrogen stress treatment was carried out around the three-leaf stage, and the barley seedl...
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