Traditional Chinese medicine extract for suppressing generation of in-vitro glycosylation product and extraction method

Abstract

The invention discloses a traditional Chinese medicine extract. The extract includes the rehmanniae praeparatum and fructus corni separated decoction aqueous extract solution aqueous extract, the rehmanniae praeparatum and fructus corni combined decoction aqueous extract solution aqueous extract, the rehmanniae praeparatum and fructus corni separated decoction and combined decoction aqueous extract drug serum, the effective parts of rehmanniae praeparatum (iridoid glycoside, oligosaccharide and polysaccharide), the effective parts of fructus corni (iridoid glycoside, polysaccharide and triterpenic acid), and the orthogonal compatibility combination of the 6 effective parts. In the effective parts, the content of catalpol in the iridoid glycoside part of the rehmanniae praeparatum is 1.91%, the purity of the polysaccharide is 68.52%, the content of two glycosides in the iridoid glycoside part of the fructus corni is 44.8%, etc. The traditional Chinese medicine extract is subjected to relatively systematic screening research by taking the in-vitro AGEs generation model as a research object, and the result indicates that the traditional Chinese medicine extract can obviously suppress the generation of in-vitro glycosylation products, namely fructosamine (early stage) and AGEs (late stage), thus the invention provides an experimental basis for clinically using rehmanniae praeparatum and fructus corni to treat the vascular diseases such as diabetic nephropathy and the like with the traditional Chinese medicine.

Description

technical field [0001] The invention relates to a class of traditional Chinese medicine extracts, in particular to a class of traditional Chinese medicine extracts for inhibiting the formation of glycosylation products in vitro, an extraction method and application. Background technique [0002] Diabetes belongs to the category of "diabetes syndrome" in traditional Chinese medicine. Diabetic vascular disease is the main complication of diabetes. The nature of its macrovascular disease is atherosclerosis, which mainly involves large blood vessels such as aorta and coronary artery. Diabetic microangiopathy is a chronic vascular complication unique to diabetes, mainly manifested as retinal , kidney and other microvascular diseases. Clinical statistics show that 40%-50% of diabetic patients develop retinopathy 10 years after onset; about 2% of patients are completely blind 15 years after onset; End-stage renal disease. Although the exact pathological mechanism of the occurren...

AI Technical Summary

Problems solved by technology

The Maillard reaction occurs on the amino groups of proteins and other substances in vitro or in vivo and the aldehyde groups of sugars. It does not require enzyme catalysis, but it is a very complicated process.

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