13 results about "TEST Mixture" patented technology

The present disclosure relates to novel methods and compositions for a measuring exonuclease enzyme activity. In particular, the methods entail contacting a sample with a fluorescently-labeled substrate to create a test mixture, incubating the test mixture for a time sufficient for cleavage of the substrate, and measuring the fluorescence signal from the test reaction mixture. In certain aspects, the methods disclosed herein are useful for identifying and / or assessing a modulator of an exonuclease that may be used either alone or in combination with other compounds. The present disclosure also relates to fluorescently-labeled double-stranded nucleic acid compositions useful in the practice of such methods and, still further, to kits for performing the methods of the disclosure. The present disclosure further relates to compounds and methods useful for treating a viral infection.

The invention relates to the technical field of soft capsule preparation, in particular to a soft capsule capable of enhancing immunity and resisting oxidation and a preparation method thereof, and the preparation method comprises the following steps: putting auxiliary materials into stirring equipment for mixing, heating and stirring; respectively detecting the stress of the stirring paddle and the linear distance between the micelles and the stirring paddle; determining a micelle bulge adhesion risk according to the stress and the linear distance; determining a rotational flow strength coefficient of an inner rotational flow area of the test mixture; determining the rotating speed of the stirring paddle; the auxiliary materials are actually mixed, heated and stirred according to the rotating speed of the stirring paddle; carrying out defoaming treatment; sequentially filtering, preserving heat and standing to output a glue solution, and cooling and shaping the glue solution to form an upper glue cap and a lower glue cap; the pressing strength of the upper rubber cap and the lower rubber cap is determined according to the strength coefficient difference value; and pressing the upper rubber cap and the lower rubber cap according to the pressing strength to form the soft capsule capable of enhancing immunity and resisting oxidation. According to the invention, the uniformity, the stability and the reliability of the product quality are improved.

The present invention relates to a competitive homogeneous chemiluminescent detection method comprising the following steps: contacting an analyte with a reagent comprising a first composition, a second composition, a third composition, and a fourth composition to form a test mixture; illuminating the test mixture at least once with excitation light; and then detecting the intensity of the resulting chemiluminescent signal to determine the presence and / or concentration of the analyte. The method utilizes "differential receptor microspheres," resulting in excellent functional sensitivity and detection range.

The present application relates to soft capsule preparation technical field, especially to a kind of soft capsules of enhancing immunity, antioxidant and preparation method thereof, preparation method includes: auxiliary material is placed into stirring equipment and is mixed heating stirring;Stress and the linear distance of glue mass and stirring paddle are detected respectively;According to stress and linear distance, determine the risk of sticking of glue mass protrusion;Determine the rotational flow intensity coefficient of inner rotational flow area of test mixture;Determine the rotating speed of stirring paddle;According to the rotating speed of stirring paddle, the actual mixing heating stirring of auxiliary material is carried out;Defoaming treatment is carried out;In turn, filtering, heat preservation and standing are carried out to output glue liquid, and glue liquid cooling shaping treatment forms upper glue cap and lower glue cap;According to the difference of intensity coefficient, the pressing strength of upper glue cap and lower glue cap is determined;According to the pressing strength, upper glue cap and lower glue cap are pressed to form the soft capsule of enhancing immunity, antioxidant.The present application realizes the promotion of the uniformity, stability and reliability of product quality.

The application discloses a detection mixture for predicting the sensitivity of PRMT5 inhibitor in treating tumors, and belongs to the technical field of biological medicine. The detection mixture comprises: a BRCA gene mutation detection agent; and / or, a DNA damage level detection agent; and / or, an IFN pathway level detection agent. The application verifies the use of the BRCA gene mutation, the DNA damage level and the IFN pathway level in the reagent for predicting the sensitivity of the PRMT5 inhibitor in preventing and / or treating solid tumors. The BRCA gene mutation detection agent, the DNA damage level detection agent or the IFN pathway level detection agent can be used for preparing the reagent for predicting the sensitivity of the PRMT5 inhibitor in preventing and / or treating solid tumors.

The present invention relates to a competitive homogeneous chemiluminescent detection method comprising the following steps: contacting an analyte with a first composition, a reagent comprising a second composition, a third composition, and a fourth composition to form a test mixture; illuminating the test mixture at least once with excitation light; and then detecting the intensity of the resulting chemiluminescent signal to determine the presence and / or concentration of the analyte. The method utilizes "differential receptor microspheres," resulting in excellent functional sensitivity and detection range.

The invention discloses bio-based thumb glue for an automobile and a preparation method of the bio-based thumb glue. The thumb glue is prepared from the following raw materials in parts by weight: 60-70 parts of a high-melting-point bio-based premix, 10-20 parts of reinforced resin, 2-5 parts of a curing agent, 5-10 parts of reinforcing filler and 20-30 parts of a rheology modifier. The method comprises the following steps: 1) putting a high-melting-point bio-based premix into a reaction kettle, heating to 50 DEG C, sequentially adding reinforced resin, a curing agent and reinforcing filler, and stirring at 75 DEG C for 30 minutes at the speed of 200 revolutions per minute to obtain a mixture; (2) when the consistency of the sampled and tested mixture is 6.0-7.5, transferring the material in the reaction kettle into a kneading machine; and step 3) adding the rheology modifier into the kneading machine, mixing for 20 minutes at 40 DEG C at the speed of 20 revolutions per minute, sampling and testing the material, and discharging when the needle penetration is in the range of 100-250, thereby obtaining the bio-based thumb glue. According to the thumb glue and the method, the dependence on traditional o-benzene and p-benzene plasticizer raw materials can be reduced on the premise that the performance is not reduced.

The invention discloses a method for detecting the content of molybdenum in a cobalt-based alloy. The method comprises the following steps: obtaining a mass m sample of a cobalt-based alloy sample; the method comprises the following steps: thermally dissolving a sample with nitric acid and hydrofluoric acid until the volume is constant to a V sample, respectively adding a molybdenum test mixed solution and a potassium cyanate-thiourea mixed solution into an obtained sample solution, a sodium hydroxide aqueous solution serving as a blank solution and a molybdenum standard solution serving as a blank solution, and carrying out a chromogenic reaction; the absorbance of the sample solution, the blank solution and the molybdenum standard solution after color development is measured, the molybdenum content in the sample is calculated through the obtained absorbance, a V sample, an m sample and the like, and the molybdenum test mixed solution comprises oxalic acid, copper sulfate and sulfuric acid. According to the method, high-precision, high-stability and wide-content-range molybdenum content measurement can be realized through simple equipment.

The application provides a mixture identification method and device based on infrared spectrum and de-peak matching, which comprises the following steps: constructing a to-be-tested characteristic peak set according to a to-be-tested infrared spectrum of a to-be-tested mixture, and constructing a sample characteristic peak set according to sample infrared spectrum of a plurality of sample substances; iteratively performing de-peak matching on the to-be-tested characteristic peak in the to-be-tested characteristic peak set and the sample characteristic peak in the sample characteristic peak set, performing similarity matching on the characteristic peaks in the to-be-tested characteristic peak set and the sample characteristic peak set corresponding to each de-peak matching, deleting the to-be-tested characteristic peak with the maximum similarity from the to-be-tested characteristic peak set after each de-peak matching, and deleting all sample characteristic peaks of the sample substance corresponding to the sample characteristic peak with the maximum similarity from the sample characteristic peak set, so as to obtain a sample substance set matched with the to-be-tested mixture, and further automatically identifying the component result of the to-be-tested mixture. The application realizes automatic and accurate mixture identification.

This application relates to a method and its application for improving the detection signal value of photo-induced chemiluminescence (PET). The method includes: mixing a companion reagent with antibody-coated luminescent microparticles, antibody-coated biotin, avidin-coated photosensitive microparticles, and a sample to be tested, reacting the mixture to generate a test mixture; exciting the test mixture at least once with excitation light, and measuring the amount of emitted photons to obtain a light signal value; wherein the companion reagent includes an oxygen-generating agent and a catalyst, the oxygen-generating agent undergoes a catalytic reaction under the action of the catalyst to generate oxygen, and the oxygen provides singlet oxygen for PET detection. The solution provided in this application can improve the detection signal value of PET, thereby enhancing the stability, sensitivity, and precision of the detection.

The present invention relates to an improved method for determining the amount of FVIII inhibitors in a patient sample, comprising the provision of a patient plasma sample, a normal pool plasma samples, and a control plasma sample which is FVIII / VWF deficient, inactivating all clotting factors present in the samples, addition of recombinant FVIII (rFVIII) to the control plasma sample, combination of the patient plasma sample with the control plasma sample with rFVIII to obtain a test mixture, and of the normal plasma pool sample with the control plasma sample with rFVIII to obtain a control mixture, incubation of the test mixture and the control mixture for less than 30 minutes, and analyzing the mixtures for residual rFVIII activity in the absence of VWF.

The invention belongs to the technical field of acetonitrile detection, and particularly discloses an acetonitrile recognition method, device, medium and equipment, the method comprises the following steps: mixing a sample to be detected with a colorimetric detection reagent to obtain a mixture to be detected; carrying out image acquisition on the mixture to be detected; preprocessing the image, and extracting an ROI (Region of Interest) of the preprocessed image to obtain an ROI (Region of Interest) image; and inputting the ROI image into an acetonitrile recognition model to judge whether the sample to be detected is acetonitrile or not and output an acetonitrile concentration value. According to the invention, rapid, objective and accurate on-site qualitative and quantitative detection of acetonitrile can be realized.

Disclosed is a method for quantitative detection of a small, aberrant viral nucleic acid. The method includes combining a detection mixture with a sample, and allowing a reaction with the test mixture to begin. The sample includes an RNA sample. The detection mixture includes a Cas13 enzyme, a reporter, a sequence-specific CRISPR RNA (crRNA), and a buffer. The method includes determining a quantitative value representative of the reporter in the test mixture, where the quantitative value is a measure of an amount of target RNA present in the RNA sample.