51 results about "TEST Mixture" patented technology

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (303) on a flowing suspension of cells (349), in which a homogeneous suspension of each member of a series of cell types (349) is combined with a concentration of a test compound (303), directed through a detection zone (355), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (355). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short timespan.

A method of characterizing a multi-component mixture for use in a bioprocess operation that includes providing a multi-component mixture standard with pre-determined amounts of known components; performing a Raman Spectroscopy analysis on the multi-component mixture standard; providing a multi-component test mixture from the bioprocess operation; performing a Raman Spectroscopy analysis on the multi-component test mixture; and comparing the analysis of the multi-component mixture standard and the multi-component test mixture to characterize the multi-component test mixture. In one embodiment, the multi-component mixture standard and the multi-component test mixture both comprise one or more of, at least two, at least three of, or each of, a polysaccharide (e.g. sucrose or mannitol), an amino acid (e.g., L-arginine, L-histidine or L-ornithine), a surfactant (e.g. polysorbate 80) and a pH buffer (e.g., a citrate formulation).

The present invention provides a simple, cost-effective, universal method for determining and/or quantifying differences in nucleic acid levels between two or more test mixtures without prior knowledge of the sequence of the nucleic acids of interest. The method involves providing a universal microarray containing a plurality of spots, where each spot contains a plurality, or pool, of different oligonucleotide probes having at least three distinct portions: a universal sequence portion, a short central variable “wobble” sequence portion, and a unique sequence portion. A set of probes is synthesized such that the universal sequence portion is the same for every probe, and all possible permutations of the wobble sequence and unique sequence portions are represented in approximately equal concentrations in the set. The probes are pooled on the universal microarray such that probes in a given spot have the same unique portion and every permutation of the short wobble, while probes on different spots have different unique portions. Primers complementary to the universal and wobble portions of the probes are used to synthesize, for example, cDNA from an mRNA preparation. The cDNA is hybridized to the universal microarray, and through the use of differential labeling, is identified and/or quantified.

In this testing device, a saliva sample and a buffer solution are delivered from separate chambers into a mixing chamber to mix with a second reagent. The resulting test mixture is allowed to incubate for a pre-determined period of time and then selectively delivered to a test strip

The invention discloses a method for determining the content of drug components in a mixture drug. The mixture drug is prepared from a first drug and a second drug. The preparation method comprises the following steps: (1) preparing a group of standard samples with different component contents; (2) collecting the terahertz time-domain spectrums of each standard sample and air, and respectively converting the terahertz time-domain spectrums into absorption coefficients and refractive indexes of corresponding frequency domain; (3) determining the terahertz band confidence interval of a test model; (4) selecting a group of optical parameters in the terahertz band confidence interval of each standard sample in the test model to be used as effective optical parameters and establishing a corresponding effective optical parameter matrix; (5) establishing a test model of the corresponding relationship between the content of the first drug and the effective optical parameters of each standard sample; and (6) inputting the effective optical parameter matrix of the sample of the to-be-tested mixture drug to the test model to obtain the content of the first drug in the to-be-tested mixture drug. By utilizing the method, the content of the drug components in the mixture drug can be quickly detected in a lossless way.

In a method of determining a regression model, and a method of predicting a component concentration of a test mixture using the regression model, the method of determining the regression model includes selecting a regression model, generating a plurality of observation points, each one of the plurality of observation points having a prediction target value and a measured value, determining a weight of the prediction target value and the measured value at each one of the plurality of observation points, and obtaining a calculation amount by reflecting the weights in differences between predicted values acquired by applying the measured values to the regression model and the prediction target values, and determining coefficients of the regression model to minimize the calculation amount.

The invention discloses a control system for a concrete raw material mixture ratio. Through the control system, the using amounts of all raw materials for a large-size concrete mixing building can be automatically controlled according to a test mixture ratio in a construction period, and thus the construction quality of concrete is improved. The control system comprises an aggregate using amount control module, a concrete water using amount control module, an ice adding amount control module, a sand using amount control module, a cement using amount control module, a coal ash using amount control module, an additive using amount control module and a master control room server. Real-time communication between the master control room server and the aggregate using amount control module, the concrete water using amount control module, the ice adding amount control module, the sand using amount control module, the cement using amount control module, the coal ash using amount control module and the additive using amount control module is conducted through networks respectively. The master control room server provides mixture ratio information for the modules according to earlier stage concrete property test results, and the using amounts of the raw materials are controlled intelligently in the concrete mixing process. The invention further discloses a control method.

Provided are methods, such as high throughput methods, of assessing or measuring the ability of an enzyme to modify the supercoil topology of a target nucleic acid, the methods comprising the steps of: (a) providing a solid support to which a capture nucleic acid is or may be immobilised, which capture nucleic acid is capable of binding the target nucleic acid in a manner that is proportional to the supercoil topology of said target nucleic acid (e.g. by triplex formation); (b) incubating a test mixture comprising (i) the enzyme, (ii) the target nucleic acid, (iii) capture nucleic acid, in the presence of (iv) said solid support, such that supercoiled target nucleic acid bound by the capture nucleic acid is immobilised to the solid support, (c) determining the amount of target nucleic acid bound by said capture nucleic acid in step (b) e.g. by use of a detectable label. The method may be used to screen for modulators of the enzyme activity.

The invention discloses a stacking forming funnel for a test of oxidation combustion characteristics of solid dangerous goods. The stacking forming funnel comprises five layers of modules stacked from bottom to top, a truncated-cone-shaped through hole is formed in the center of each of the lower four layers of modules, the truncated-cone-shaped through holes are arranged from large to small from bottom to top, a conical counter hole is formed in the center of the five layer of module and communicated with all the truncated-cone-shaped through holes, and the holes are combined to form a conical funnel hole. The stacking forming funnel not only realizes bottom surface consistent stacking forming of tested mixtures with inconsistent volumes, but also realizes consistency of stacking forming of the tested mixtures with the same volumes under the condition that the overall funnel cannot be filled with the tested mixtures in volume, and accordingly, the reproducibility of the test of the oxidation combustion characteristics of the solid dangerous goods is guaranteed, and a forceful guarantee is provided for the strict test of the oxidation combustion characteristics of the solid dangerous goods according to requirements; besides, the stacking forming funnel is simple in structure and low in cost and can realize direct output through 3D printing.

A method of measuring the gas flow of an incondensable gas in a flowing mixture of said incondensable gas and a condensable gas comprising the steps of calibrating a leak detector; injecting a known quantity of gauge gas at a known mass flow rate into said flowing mixture of gases; drawing a sample of the said gauge mix off at a sample point; passing said sample through a condenser so as to remove said condensable gas from the gauge mix, resulting in a test mix; passing the said test mix into the leak detector so as to measure the concentration of the gauge gas within the test mix; and calculating the mass flow rate of said incondensable gas within said flowing mixture. There is also provided a method of measuring the rate of ingress of an incondensable gas at an ingress point along a flow path of a flowing mixture of said incondensable gas and a condensable gas.

The present invention relates to a fixing member having high abrasion resistance and being excellent in toner releasability, and a method of manufacturing the fixing member. The fixing member according to the present invention includes: a base member; an elastic layer formed on a surface of the base member; and a surface layer, in which the surface layer contains a tetrafluoroethylene-hexafluoropropylene copolymer having a specific partial structure and has a surface that forms a contact angle of 65° or more with respect to a wetting tension test mixture having a wetting tension of 31.0 mN/m.

The present invention relates to methods for detecting or quantifying an analyte in a test sample including providing at least one test mixture including a test sample, at least one marker complex, wherein each marker complex includes a particle, a marker, and one member of a coupling group, a first binding material selected to bind to a portion of the analyte, a second binding material selected to bind with a portion of the analyte other than the portion of the analyte for which the first binding material is selected, analyte analog, and/or marker conjugate. The at least one test mixture is passed through a membrane. The amount of marker on the membrane is detected and correlated to the presence or amount of analyte in the test sample.

Methods, compositions, and kits for separating heteroduplex and homoduplex DNA molecules in a test mixture by temperature-compression denaturing high performance liquid chromatography (tcDHPLC). The method includes use of nitrogen-containing additives in the mobile phase that allow detection of diverse heteroduplex molecules to be performed at the same pre-selected temperature. An example of a preferred additive is betaine. Standard mixtures of DNA fragments, such as mutation standards containing known heteroduplex and homoduplex molecules, can be used to select the concentration of additive and temperature. Compositions and kits including the mobile phase, mutation standards, PCR primers, separation media, and DNA polymerase are also provided.

The invention relates to a testing method, in particular to a method for improving the test precision of the cement dosage for stabilizing a macadam base. The technical scheme of the method for improving the test precision of the cement dosage for stabilizing the macadam base is as follows: mixtures of multiple proportions pass through sieve pores of 13.2 mm, a series of standard samples are prepared, testing is conducted through an EDTA method, and then a standard curve is drawn; and a to-be-tested mixture is selected to pass through the sieve pores of 13.2 mm, a to-be-tested sample is prepared, testing is conducted through the EDTA method, and the cement dosage of the to-be-tested sample is obtained by comparing with the standard curve. According to the method for improving the test precision of the cement dosage for stabilizing the macadam base, the influence of artificial sampling factors on the test result can be lowered, and thus the control precision of the cement dosage is improved.

The present invention includes methods of determining the volume of liquid dispensed by a dispenser. Initially, a refractive index analysis is preformed on a first liquid that has been dispensed from the dispenser in question. From the refractive index analysis, information is obtained about the volume of first liquid dispensed. The invention also includes methods for verifying the volumetric accuracy of a dispenser. The methods include dispensing a first liquid having a known refractive index into a vessel having a second liquid with a known volume and a known refractive index to form a test mixture. The refractive index of the test mixture is measured and then correlated with the refractive index of the first liquid to obtain information about the volume of first liquid dispensed. The dispensed volume is then compared to the theoretical volume of liquid dispensed by the dispenser.