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Kit for identifying new coronavirus by applying mass spectrum system and using method thereof

A mass spectrometry system and kit technology, which is applied in the field of kits for the identification of new coronaviruses using mass spectrometry systems, and can solve problems such as inconvenience

Active Publication Date: 2020-10-27
北京东西分析仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In summary, there are obviously inconveniences and defects in the actual use of the existing technology, so it is necessary to improve

Method used

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  • Kit for identifying new coronavirus by applying mass spectrum system and using method thereof
  • Kit for identifying new coronavirus by applying mass spectrum system and using method thereof
  • Kit for identifying new coronavirus by applying mass spectrum system and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 Distinguish between novel coronavirus pneumonia and healthy people

[0074] Kit ingredients:

[0075] The preparation method of buffer U9 is: add urea and CHAPS to 0.01mol / L PBS, stir and dissolve to obtain buffer U9; the molar concentration of urea is 7mol / L, and the mass concentration of CHAPS is 8g / L.

[0076] The pH of buffer U1 is 7.5, and buffer U1 is a mixture of buffer U9 and 45mmol / L Tris-HCl.

[0077] The pH of the sodium acetate buffer is 4.0, and the molar concentration of the sodium acetate buffer is 90mmol / L.

[0078] Experimental steps:

[0079] S1 activates metalloprotein chip target plate

[0080] S2 Pretreatment of positive and negative serum samples of novel coronavirus

[0081] a) taking out the serum sample, inactivating it in a water bath after thawing; centrifuging the serum sample to separate the serum;

[0082] b) Take 20 μl of upper serum, add 30 μl of buffer U9, mix well, shake and incubate for 20 minutes to obtain intermediate ...

Embodiment 2

[0095] Example 2 Distinguish between novel coronavirus pneumonia and common pneumonia

[0096] Kit ingredients:

[0097] The preparation method of buffer U9 is as follows: add urea and CHAPS to 0.01mol / L PBS, stir and dissolve to obtain buffer U9; the molar concentration of urea is 8mol / L, and the mass concentration of CHAPS is 10g / L.

[0098] The pH of buffer U1 is 7.5, and buffer U1 is a mixture of buffer U9 and 50 mmol / L Tris-HCl.

[0099] The pH of the sodium acetate buffer is 4.0, and the molar concentration of the sodium acetate buffer is 105 mmol / L.

[0100] Experimental steps:

[0101] S1 activates metalloprotein chip target plate

[0102] S2 Pretreatment of positive and negative serum samples of novel coronavirus

[0103] a) taking out the serum sample, inactivating it in a water bath after thawing; centrifuging the serum sample to separate the serum;

[0104] b) Take 40 μl of upper serum, add 60 μl of buffer U9, mix well, shake and incubate for 20 minutes to obt...

Embodiment 3

[0117] Example 3 Distinguish between novel coronavirus pneumonia and scrub typhus

[0118] Kit ingredients:

[0119] The preparation method of buffer U9 is as follows: add urea and CHAPS to 0.01mol / L PBS, stir and dissolve to obtain buffer U9; the molar concentration of urea is 10mol / L, and the mass concentration of CHAPS is 12g / L.

[0120] The pH of buffer U1 is 7.5, and buffer U1 is a mixture of buffer U9 and 60 mmol / L Tris-HCl.

[0121] The pH of the sodium acetate buffer is 4.0, and the molar concentration of the sodium acetate buffer is 120mmol / L.

[0122] Experimental steps:

[0123] S1 activates metalloprotein chip target plate

[0124] S2 Pretreatment of positive and negative serum samples of novel coronavirus

[0125] a) taking out the serum sample, inactivating it in a water bath after thawing; centrifuging the serum sample to separate the serum;

[0126] b) Take 50 μl of upper serum, add 100 μl of buffer U9, mix well, shake and incubate for 20 minutes to obtain...

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Abstract

The invention discloses a kit for identifying a new coronavirus by applying a mass spectrum system and a use method of the kit. The kit comprises the following components: a U9 buffer solution, a U1 buffer solution and a sodium acetate buffer solution. The using method of the kit comprises the following steps: S1, activating a metalloprotein chip target plate; S2, pretreating a serum sample; S3, extracting protein in the serum sample by using the metalloprotein chip target plate; and S4, acquiring and processing a spectrogram by using an MALDI-TOF mass spectrum system, and analyzing the read spectrogram by using software. Therefore, the kit is high in sensitivity, good in accuracy, simple and convenient to operate, short in consumed time and very suitable for clinical diagnosis.

Description

technical field [0001] The invention relates to the technical field of novel coronavirus detection, in particular to a kit for identifying novel coronavirus using a mass spectrometry system and a method for using the same. Background technique [0002] Novel coronavirus (COVID-19) is a virus that seriously threatens the lives and health of the people. How to diagnose the virus faster and more accurately has become a hot spot of concern from all walks of life. Currently, there are two main methods of detecting the new coronavirus: [0003] One is the nucleic acid detection method, that is, the real-time fluorescent RT-PCR method. Due to various reasons such as sampling location, sampling volume, transportation and storage links, laboratory testing conditions and personnel operations, fluorescent quantitative RT-PCR is used as a detection method The positive rate of the new coronavirus test is currently only 30%-50%, resulting in an extremely high false negative rate. At pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/28G01N1/34G01N1/40
CPCG01N33/6851G01N1/28G01N1/34G01N1/40Y02A50/30
Inventor 马龙华李晓雯李宁顾好粮高利艳张丹丹赵庆军孙斌谢文君王晓东刘松坡王利龙皎杨丽华
Owner 北京东西分析仪器有限公司
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