48 results about "ProteinChips" patented technology

The invention belongs to the field of biomedical detection, and particularly relates to a protein chip, a detection kit based on a polypeptide coding microarray chip and application of the detection kit. According to the protein chip provided by the invention, an antibody and a bispecific antibody of corresponding indexes can be added into a treating fluid according to detection requirements, the antibody and the bispecific antibody are combined with the chip in a manner of polypeptide specific recognition after being subjected to antigen specific recognition respectively, and joint detection of different antigen combinations and detection of a single index are carried out. By utilizing the polypeptide sample application chip and the antigen and antibody combined bispecific antibody provided by the invention, the universality of the chip in use, the high efficiency of detection and the low cost can be remarkably improved.

The invention relates to the technical field of machine vision detection, in particular to a protein chip automatic quality inspection method based on machine vision and related equipment.The method comprises the steps that parameter information and image data are obtained, communication connection is established, mechanical motion errors are compensated through datum point positioning, images are preprocessed and spliced, and defect types are recognized. And outputting an analysis conclusion and establishing closed-loop feedback and data tracing. According to the method, the protein chip quality inspection automation is realized, the detection precision and efficiency are improved, the problems of large error, low efficiency and data non-traceability in traditional manual detection are solved, and reliable quality control technical support is provided for industrial production.

The invention discloses a liquid-phase protein chip method for simultaneously detecting three kinds of porcine coronaviruses and a kit thereof, which comprises the following steps of: after activating and coupling microspheres, respectively coating antigen on porcine delta coronavirus N protein, porcine acute diarrhea syndrome coronavirus N protein and porcine epidemic diarrhea virus N protein by using the microspheres; matching the two fluorescent dyes according to different concentrations to form fluorescent coding microsphere combination arrays with different energy levels; the microspheres are analyzed one by one based on the flow principle, red laser recognizes microsphere codes, green laser reads fluorescence intensity of report molecules, computer analysis and processing are carried out, and fluorescence signals are converted into quantitative detection results; and determining the optimization of the detection conditions of the liquid-phase protein chip after the liquid-phase protein chip test. The antigens coated on the microspheres only specifically react with corresponding antibodies and are not combined with antibodies generated by other pathogens; the PDCoV, SADS-CoV and PEDV antibodies in the sample to be detected can be rapidly, effectively and accurately detected, and reference is provided for prevention of the porcine coronavirus.

The invention relates to a confining liquid, a preparation method of a protein chip and a method for improving point-shaped trailing of the protein chip. The confining liquid comprises the following components: 0.1% v / v-0. 5% v / v of triton X-100, 10 g / L-100 g / L of cane sugar, 0.1 mol / L-0. 2 mol / L of ammonium sulfate, 0.01% v / v-0. 1% v / v of a biological buffer solution, 5% v / v-15% v / v of goat serum and 0.05 g / L-1 g / L of protamine, and the pH value of the confining liquid is 6.5-7.5. After the sample application of the protein chip is completed, the chip is sealed by using the sealing liquid, so that the point shape trailing of the protein chip can be improved.

The invention discloses a method for carrying out protein chip detection on brucellosis, and relates to the technical field of brucellosis detection. The preparation method comprises the following steps: spotting a Brucella synergistic composite antigen for multi-index joint detection on a pretreated solid-phase carrier, drying and washing; then adding a sealing agent for sealing, and washing to obtain a protein chip; then collecting an initial sample, diluting and centrifuging, and taking supernate; adding the supernate to a protein chip site, oscillating, incubating, washing, adding a secondary antibody, further incubating and washing; and finally, reading the signal by using a fluorescence scanner, deducting the background, and interpreting the positive and negative properties. The method is wide in detection range, strong in specificity and high in sensitivity, and has a very wide application prospect.

The invention relates to an ultra-sensitive protein chip and a kit for detecting influenza A H3 (H3N2) hemagglutinin protein, which belong to a small molecular marker detection technology and are characterized in that the protein chip is provided with at least one sub-detection area, each sub-detection area is provided with at least one detection spot, and the detection spots are provided with non-overlapping, dispersed and tiled and non-overlapping detection spots. The coating density is 1.5 * 10 < 11 > capture molecules per square millimeter; the capture molecule is a testosterone monoclonal antibody. The testosterone protein chip disclosed by the invention can be used for rapidly detecting influenza A H3 (H3N2) hemagglutinin protein as low as 125fg / ml at low cost, and is beneficial to improving the early and correct diagnosis of influenza A.

The invention belongs to the technical field of Alzheimer's disease marker detection, and particularly relates to an Alzheimer's disease blood marker joint detection kit and a manufacturing method thereof.The kit comprises a protein chip, the protein chip comprises a chip base and marker antibodies coating the chip base, the marker antibodies comprise anti-A beta (1-40) IgG, anti-A beta (1-42) IgG, anti-T-Tau IgG, anti-P-Tau181IgG, anti-P-Tau217 IgG and anti-S100 beta IgG, the chip base is coated with the marker antibodies, and the chip base is coated with the marker antibodies. The Alzheimer's disease blood marker joint detection kit is high in sensitivity, specificity and accuracy, stable in result, good in repeatability and convenient and fast to operate, saves manpower and material resources, is a reliable tool means for early diagnosis of Alzheimer's disease by medical workers, and improves the labor efficiency. The patient can be treated earlier, and the survival rate is increased.

The invention belongs to the field of medicine detection, and particularly discloses a comprehensive control method for the quality of compound Pien Tze Huang lozenges, which comprises the following steps of: fishing targeted protein active ingredients of the compound Pien Tze Huang lozenges by respectively adopting a surface plasma resonance instrument with a built-in TNFR2 protein chip, a built-in TLR4 protein chip or a built-in TLR5 protein chip; the biological effect of the compound Pien Tze Huang lozenge is detected by adopting an SPR method based on the fished anti-inflammatory active ingredients, and the fingerprint spectrum of the compound Pien Tze Huang lozenge and the content of the ingredients in the fingerprint spectrum are detected by adopting an UPLC method. The surface plasmon resonance method and the UPLC method are combined to control the quality of the compound Pien Tze Huang lozenge, evaluation of the overall drug effect and accurate determination of component composition are combined, the quality of the compound Pien Tze Huang lozenge is controlled more comprehensively, and the method has practical application value.

The present invention discloses a Helicobacter pylori whole protein chip and a preparation method and application thereof, wherein the Helicobacter pylori whole protein chip comprises a substrate and 1631 kinds of Helicobacter pylori proteins connected thereto. The Helicobacter pylori whole protein chip prepared by the present invention can be used to screen Helicobacter pylori proteins that interact with small molecule compounds, and has the characteristics of global and high-throughput screening, simplifies the research design and operation process, and significantly improves the research efficiency.

The invention discloses an African swine fever virus whole-protein chip and application thereof. The invention provides a protein chip with at least one of the following functions (1)-(2), 160 proteins are connected to the protein chip, and each protein independently forms a detection point; experiments prove that the invention provides an African swine fever virus whole-protein chip which is composed of a substrate and 160 proteins coded by more than 95% of African swine fever virus genes. The whole-protein chip has obvious significance in discovery of protective antigens of the African swine fever virus and research of interaction between the virus and a host. Compared with an existing research means, the method has the advantages of being global and high in throughput screening, the research design and operation process is simplified, and the research efficiency is remarkably improved.

The utility model relates to the technical field of biology, and discloses a biological protein chip reaction instrument which comprises a reaction instrument rack, a delta moving mechanism is arranged on the reaction instrument rack, the delta moving mechanism is connected with a moving execution piece, the rack comprises a base and a top sealing plate, a control device is arranged in the base, and the top sealing plate is connected with the control device. A regulation and control assembly is arranged on the front side face of the base, a working platform is fixed to the center of the top face of the base, three sets of magnetic attraction clamping pieces are fixed to the base with the working platform as the center at intervals of 120 degrees, and the magnetic attraction clamping pieces magnetically attract and clamp a sample application executing mechanism, a CCD chip scanning mechanism and a reagent clamping mechanism. The biological protein chip reaction instrument solves the problems that an existing biological protein chip reaction instrument generally adopts a portal frame structure or an XYZ box body structure to carry out three-dimensional space movement of an execution device, but the portal frame structure or the XYZ box body structure is low in movement speed and easy to shake, so that reagents are polluted.

The present invention, "Alkaline Phosphatase Protein Chip Kit for Detecting Novel Coronavirus Antibodies and Its Preparation Method", relates to virus detection technology, and is characterized in that it has at least one protein chip, each protein chip has at least one sub-detection area, and each sub-detection area is used to detect one specimen; the following several detection spots are arranged in each sub-detection area: the first detection spot formed by the attachment of BSA, the second detection spot formed by the attachment of novel coronavirus antigen, and the third detection spot formed by the attachment of human IgG polyclonal antibody or IgM polyclonal antibody; the detection spots are separated from each other; the novel coronavirus antigen is the receptor-binding domain protein of the S1 subunit of the novel coronavirus spike protein or the S1 subunit protein of the novel coronavirus spike protein, or a mixture thereof.

The present invention relates to a kind of Angong Niuhuang Wan quality evaluation method based on STAT3 protein biological effect, it is to use surface plasmon resonance (SPR) detection technology, specifically including the following steps: 1) construction of protein chip;2) preparation of test solution;3) preparation of reference solution;4) test solution and reference solution injection are taken respectively and carry out SPR detection, analyze and flow through protein chip surface and STAT 3 protein binding, measure corresponding response value (RU value).Quality evaluation standard is that the ratio of test sample RU value to reference substance RU value should be not less than 1.The present invention is optimized and verified by methodology, and accuracy is high, can reflect the effectiveness of Angong Niuhuang Wan clinical treatment, further improves the quality control system of Angong Niuhuang Wan, possesses practical application promotion value.

The invention belongs to the field of drug detection, and particularly discloses a method for detecting a biological effect of a Xinshu tablet, which comprises the following steps of: fishing targeted protein active ingredients of the Xinshu tablet by adopting a surface plasma resonance instrument with a built-in TGF-beta protein chip, and detecting the biological effect of the Xinshu tablet by adopting an SPR (Surface Plasmon Resonance) method based on a fished biological quality marker. According to the method, the quality of the Xinshu tablet is controlled by adopting a surface plasmon resonance method, evaluation of the overall drug effect and composition are combined, the quality of the Xinshu tablet is controlled more comprehensively, and the method has practical application value.

The utility model relates to the field of automatic product quality inspection, in particular to an automatic protein chip quality inspection device based on machine vision. Comprising the steps that an X-axis moving platform drives a camera to horizontally move in the X-axis direction, a Y-axis moving platform drives a bearing tray to horizontally move in the Y-axis direction, the camera is fixed to the X-axis moving platform, the bearing tray is used for bearing a protein chip, and the camera is in communication connection with a PC terminal to transmit image data; a positioning calibration module is further arranged to compensate mechanical errors, a light source module provides a detection light source, and the surface of the bearing tray is provided with an anti-skid coating and / or a positioning groove. And the PC end configures an image splicing control module to splice the local images of the blocks into a global view in combination with the position encoder. The technical effects that automatic quality inspection of the protein chip is achieved, the quality inspection efficiency and accuracy are improved, the stability of the chip during movement is ensured, and a complete chip image is obtained are achieved.

The present invention discloses a detection product and method for determining botulinum toxin activity or synaptosome-related proteins. The detection product includes a kit, a test strip or a protein chip, which is used to detect SNAP25 or to determine botulinum toxin activity, wherein the detection product comprises an antibody or an antigen-binding fragment thereof that binds to SNAP25. The present invention achieves complete control of the experiment at each stage through phage display technology, the experimental cycle is relatively short, a large-capacity antibody library is generated, and different types of antibodies can be recombinantly produced. The antibody prepared by the present invention has good specificity and an affinity that is superior to commercial animal-derived SNAP25 polyclonal antibodies, and can be used for the detection of botulinum toxin endopeptidase activity, as well as the detection of SNAP25 in samples such as blood, cerebrospinal fluid, and exosomes.

The utility model discloses a fixing device of a protein detection chip, which comprises a table body, the top end of the table body is fixedly connected with a table top, the top end of the table top is fixedly provided with a placing table, two sides of the top end of the table top are fixedly provided with two clamping parts, and the rear part of the top end of the table top is provided with a moving mechanism. According to the fixing device for the protein detection chip, the clamping part is arranged, so that the protein detection chip can be clamped and fixed, the size and the thickness of the chip can be adjusted, and the length of the telescopic end of the air cylinder is adjusted to meet the requirement for clamping chips of different sizes; the vertical sliding of the upper clamping block is adjusted to meet the requirement for clamping chips with different thicknesses, higher adaptive capacity is achieved to adapt to protein detection chips with different specifications, the optical detector can be moved to any point above the placement table by arranging the moving mechanism, the optical detector has higher moving freedom degree, and the detection efficiency is improved. Meanwhile, the detection work of protein chips with different specifications can be met.

A group of gene clusters capable of evaluating the molecular typing and survival risk of colorectal cancer is disclosed; the application of a reagent for detecting the gene expression levels of the gene clusters in the preparation of a product for determining the molecular typing of colorectal cancer and evaluating the survival risk of colorectal cancer patients is disclosed; the product includes a next-generation sequencing (NGS) detection kit, a fluorescence quantitative PCR detection kit, a gene chip, and a protein chip. A method for molecular typing of colorectal cancer and survival risk assessment using the detection kit is also disclosed.

The present invention discloses an EBV whole protein chip and a preparation method and application thereof, wherein the EBV whole protein chip comprises a substrate and 85 EBV proteins connected thereto. The EBV whole protein chip prepared by the present invention can be used to screen EBV proteins that interact with serum antibodies, and has the characteristics of global and high-throughput screening, simplifies the research design and operation process, and significantly improves the research efficiency.

The application discloses an application of an indole dione piperazine compound in preparation of a drug for resisting prostate cancer. Experiments such as MTT, flow cytometry, protein chip, molecular docking, CETSA, transmission electron microscopy and zebra fish model prove that the compound significantly inhibits proliferation of prostate tumor cells such as PC-3, 22Rv1 and DU145, blocks division of PC-3 cells in the G1 phase, and induces apoptosis and necrotic apoptosis of PC-3 cells; the mechanism is that the compound directly combines NQO1 and NRF2, activates the HO-1 / NRF2 / NQO1 pathway, promotes excessive generation of ROS, and causes NQO1-dependent oxidative stress. The zebra fish model in vivo shows that the compound inhibits growth of prostate tumors. The application provides a lead compound with a new mechanism for preventing and treating prostate cancer and potential for overcoming drug resistance.

The utility model discloses a microfluidic protein chip, which belongs to the technical field of biochips and diagnostic reagents and comprises an upper shell and a lower shell, the upper shell and the lower shell are connected in a clamping manner, a sample introduction window is arranged on the upper shell, and a through hole is arranged on the upper shell; a sample adding area, a reaction area and a micro-fluidic area are arranged in the lower shell, the sample adding area is located at one end of the reaction area, and the micro-fluidic area is arranged at the other end of the reaction area; the micro-fluidic area comprises a micro-fluidic channel and a waste liquid tank, and after the upper shell and the lower shell are covered, the through hole is formed in the upper part of the waste liquid tank. The micro-fluidic protein chip disclosed by the utility model is simple to manufacture and convenient to operate, not only can accurately control the reaction time, but also can realize the recovery of waste liquid and avoid environmental pollution.

The invention belongs to the technical field of biology, and discloses a gene group for breast cancer survival risk assessment. The invention also discloses application of the reagent for detecting the gene expression level of the gene group in preparation of a product, and the product is used for evaluating the survival risk of a breast cancer subject. The product comprises a fluorescent quantitative PCR detection kit, a next generation sequencing (NGS) detection kit, a gene chip and a protein chip. The invention also discloses a method for evaluating the survival risk of breast cancer by using the gene group or the detection kit.

The present invention provides the preparation and application of a visual positioning solid-phase protein chip. By using a protein fixing solution containing water-soluble dyes, surfactants, water-soluble high molecular polymers and other components, the protein is fixed on a specific solid-phase carrier to obtain a visual positioning solid-phase protein chip that does not require sealing or cleaning; and in the process of using the solid-phase protein chip for measurement, the water-soluble dye will be completely removed, and will not affect the signal reading of the analyte detection process at all. The visual positioning solid-phase protein chip provided by the present invention is convenient for quality control of the solid-phase protein chip and simultaneous analysis of a large number of indicators due to visual positioning, which reduces the manufacturing difficulty of the solid-phase protein chip and improves the quality, and is very worthy of promotion and application.

The invention provides a lung tumor early warning protein chip and application thereof. The protein chip comprises an antigen group, the antigen group comprises an antigen; the antibody corresponding to the antigen is p53, NY-ESO-1, CAGE, GBU4-5, SOX2, HuD, MAGE A4, SARS, ZPR1, FAM131A, GGA3, PRKCZ, HDAC1, GOLPH3, NSG1, CD84, EEA1, CEA, CYFRA211 and the like; the protein chip is used for early warning and evaluation of lung tumors, and the early warning and evaluation comprises the following steps: collecting serum of a to-be-detected crowd, detecting the serum by using the protein chip to obtain detection data, and then judging whether the to-be-detected crowd belongs to a low-risk, medium-risk or high-risk crowd according to a risk early warning model; the protein chip systematically detects tumor-related autoantibody risk factor signals by a high-throughput technology, and is used for risk early warning detection of lung tumors.

The utility model provides a full-automatic sample application device for a precise microarray type protein chip, which comprises a supporting table, a movable base table and a flexible film carrying sheet, and is characterized by further comprising an automatic sample application mechanism which comprises a sample application structure, a container for storing protein liquid, an automatic valve switch assembly, a two-dimensional movable assembly and a sample applicator with a liquid outlet hole, the position recognition structure is provided with a position recognition emitter and a plurality of position recognition receivers, and the circuit substrate is electrically connected with the automatic valve switch assembly, the two-dimensional moving assembly, the recognition emitter and the position recognition receivers. Due to the adoption of the structure and the arrangement of the position identification structure in the automatic sample application mechanism, compared with the existing manual sample application, the automatic sample application mechanism has the advantages that the sample application position can be accurately detected, the full-automatic function is realized in the sample application process, the full-automatic sample application is realized, the production efficiency is high, the sample application positioning is accurate, and the consumed time is short.

The invention belongs to the technical field of autoimmune encephalitis, and particularly relates to an autoimmune encephalitis biomarker combined detection protein chip and a kit, the protein chip comprises a substrate and a marker antibody coated in the substrate, the substrate is provided with at least one sample application module, the sample application module is provided with the marker antibody, and the marker antibody is coated in the substrate. The kit comprises a protein chip, a sample diluent, a cleaning buffer solution and fluorescent group labeled goat anti-human IgG, and the autoimmune encephalitis biomarker combined detection protein chip and kit have the advantages of high sensitivity, strong specificity, high accuracy, stable result, good repeatability, convenient and fast operation, manpower and material resource saving, and high detection efficiency. The kit is a reliable tool means for early diagnosis of autoimmune encephalitis by medical workers, and the labor efficiency is improved. The patient can be treated earlier, and the survival rate is increased.

The utility model discloses a microporous percolation protein detection chip, which belongs to the technical field of biochips and diagnostic reagents, and comprises an upper shell and a lower shell, the upper shell is connected with the lower shell in a clamping manner, a microporous membrane is arranged between the upper shell and the lower shell, the upper surface of the upper shell is provided with a concave cavity, a sample introduction window is arranged in the cavity, and the sample introduction window is connected with the microporous membrane. A sample introduction window is formed in the upper surface of the lower shell, a sample introduction hole is formed in the sample introduction window, the lower end of the sample introduction hole is connected with a sample introduction channel, the bottom of the sample introduction channel is connected with a reaction area on the microporous membrane, the lower end of the reaction area is connected with a sample outlet channel, and the lower end of the sample outlet channel is connected with a sample outlet hole in the upper surface of the lower shell. The protein chip disclosed by the utility model can reduce the use amount of samples, is accurate in measurement result, and does not cause environmental pollution.

This invention discloses a method and system for identifying the origin and year of dried tangerine peel based on multi-biomimetic sensor fusion. The method includes: acquiring at least two biosensor response signals of the tangerine peel to be tested, wherein the at least two biosensor response signals include a first response signal from an olfactory receptor protein chip and a second response signal from a neuron chip; inputting the first and second response signals into a pre-trained first classification model and a second classification model respectively to obtain a first and second predicted origin and / or year corresponding to the tangerine peel to be tested; fusing the first and second predicted origin and / or year results, and outputting the origin and / or year identification result of the tangerine peel to be tested based on the fused result. This invention significantly improves the accuracy, reliability, and reproducibility of identifying the origin and year of dried tangerine peel, and achieves rapid, non-destructive on-site detection.

The invention belongs to the technical field of biological prediction reagents, and provides application of solute carrier protein SLC7A2 in preparation of a reagent for predicting abnormal coagulation of non-small cell lung cancer and a related product aiming at the technical problem of poor prediction capability of an existing method for detecting abnormal coagulation of a lung cancer patient. The invention discloses application of solute carrier protein SLC7A2 in predicting abnormal coagulation of non-small cell lung cancer for the first time, including preparation of a prediction reagent / kit and a protein chip for predicting abnormal coagulation of non-small cell lung cancer. By researching the relationship between the expression of the solute carrier protein SLC7A2 and the abnormal coagulation of the non-small cell lung cancer, the strong correlation between the expression of the solute carrier protein SLC7A2 and the abnormal coagulation probability of the non-small cell lung cancer is disclosed, and the guiding effect of the solute carrier protein SLC7A2 in prediction, research, diagnosis and treatment of the abnormal coagulation of the non-small cell lung cancer is defined. The method is of great significance to more effective prediction of molecular indexes of abnormal blood coagulation and thrombosis of non-small cell lung cancer patients.

The application provides a protein chip for early warning of breast cancer and application thereof, the protein chip comprises an antigen group; the antigen group comprises antigens; antibodies corresponding to the antigens are at least one of 111 kinds of AKAP4, BRCA2, CIP2A, GIPC1, P53, SOX2, A1AT, ANGPTL4, BRAF, C1D, LRRC26 and the like; the protein chip is used for early warning and evaluation of breast cancer; steps of the early warning and evaluation include collecting serum of a to-be-tested crowd, detecting the serum by using the protein chip to obtain detection data, and then judging whether the to-be-tested crowd belongs to a low-risk, medium-risk or high-risk crowd according to a risk early warning model; the protein chip systematically detects tumor-related autoantibody risk factor signals by using a high-throughput technology, and is used for risk early warning detection of breast cancer.