95 results about "ProteinChips" patented technology

This invention relates to preparation and detect method of detection protein chip of diabetes autoimmune antibody. The scheme is as follows: a protein chip is a thin glass chip set with multiple reaction tanks on top surface of the said shell and a detection microarray in each of the reaction tank, every micro-array is composed of three different kinds, same size protein micro-solid phase detection chips of GAP, PTP and Insulin. The preparation method is to prepare protein micro-solid phase detection chips to be assembled and the detecting method is to apply indirect fluorescent method for detection.

The present invention discloses a protein chip fully-automated high-throughput analysis apparatus, which comprises a control host machine, a display electrically connected to the control host machine, and a fully-automatic protein chip workstation, wherein the table surface of the fully-automatic protein chip workstation is provided with a sample treatment, a reagent treatment system, a plate washing system and a signal acquisition system, a free mechanical arm and a liquid adding system are added above the table surface of the work station, and the sample treatment system comprises a suction head support frame, a sample support frame, a reagent support frame and a barcode scanner. With the protein chip analysis apparatus of the present invention, the simultaneous detection on the multiple samples, the standard substance and the quality control substance can be achieved, the calculation on the concentration of the current batch of the samples through the current batch of the standard substance and the quality control substance is achieved, and the detection result is accurate and stable; and the multiple samples share the one standard substance or quality control substance so as to achieve the agent saving effect.

The present invention relates to the biologic technology field, and discloses a liquid phase albumen chip and the preparation and the usage method thereof which can simultaneously test human serum carcinoma embryonic antigen (CEA), Alpha fetoprotein (AFP), and hepatitis B surface antigen (HBsAg). The present invention couples the specificity antibody of CEA, AFP, and HBsAg on different fluorescence micro-spheres, and uses the test antibody marked by biotin or phycoerythrin to determine the nature quickly and quantitatively analyze the above three indexes with the double antibody sandwich method. The present invention uses the filtering membrane board when testing, and washes the board 3 times after each reaction is finished to increase the signal and improve the sensitivity. The present invention has high sensitivity, strong specificity, stable result, excellent repeatability, and simple operation; 1 micro liter serum sample can test three indexed simultaneously. The present invention is applicable to the health test and the general examination as well as the clinic test of the high risk population, and can facilitate the early diagnosis and the early treatment of the knub.

The invention discloses a chip for diagnosing the proteins. The antigens corresponding to multiple relevant indexes are printed on the different positions of the membrane of cellulose nitrate. The multi-tape immunoblotting method is utilzied through the coloration of gold mark or the precipitation enzyme without coloration to detect the antibody in the specimen of the sufferer aiming at the antibodies all antigens. The invention raises the detecting sensitivity and the specificity of the immunizing method so as to avoid the false negative or the false positive, providing the results for detecting multiple antigens in one one-stroke detection. Since the chip possesses more information than the information in the testing sheet so as to reduce the collecting quantity and shorten the testing period.

The invention relates to a protein chip, a protein chip diagnostic kit, a preparation method and a using method. The protein chip contains the following protein markers of autologous antigen protein: a P53 antigen fragment, an SOX2 antigen fragment, a COPB1 antigen fragment, an EFHD2 antigen fragment, an EIF4G3 antigen fragment and a PCNA antigen fragment, wherein the characteristic amino acid sequence of the P53 antigen fragment is as shown in ID.1sequence; the characteristic amino acid sequence of the SOX2 antigen fragment is as shown in ID.2sequence; the characteristic amino acid sequence of the COPB1 antigen fragment is as shown in ID.3sequence; the characteristic amino acid sequence of the EFHD2 fragment is as shown in picture 4seeqeunce; the characteristic amino acid sequence of the EIF4G3 antigen fragment is as shown in ID.5seqeunce; the characteristic amino acid sequence of the PCNA antigen fragment is as shown in ID.6sequence. Early diagnosis or general investigation screening for suspected patients of lung cancer or high-risk population of lung cancer can be carried out extremely conveniently, quickly, noninvasively and efficiently.

The invention proposes a novel filtering protein chip technology. The chip includes a porous cellulose filter carrier, and a microarray formed on the surface of the carrier by a plurality of capture molecules specific to the analyte. Capture molecules can be proteins (including monoclonal or polyclonal antibodies, antigens or other proteins), aptamers or other small molecules. In addition, the present invention also includes a device and an experimental method for simultaneously using multiple stacked microarray chips to detect analytes. In short, the filter-type protein chip is a new technology that is more sensitive, more specific, more quantitative, faster and higher throughput, and can be used for many applications such as multi-molecule joint detection of diseases.

The invention discloses a high flux detection method and detection biochip based on nucleic acid address coding. According to the invention, the principle of specific complementation and hybridization of nucleic acid is ingeniously utilized to locate nucleic acid with different sequences at different areas of the chip; conjugates of complementary nucleic acid and proteins are located at corresponding positions on the biochip because of interaction among nucleic acid; therefore, disadvantages and deficiency of conventional protein chips are overcome, a whole reaction is carried out in a liquid environment, and since genetic coding is infinite, limitation of bead coding in liquid phase chips is overcome. Thus, the detection biochip provided in the invention combines the advantages of solid phase chips and liquid phase chips and enables a more accurate high flux detection reaction to be achieved.

The invention discloses application of a serum molecular marker combination as a lung cancer diagnosis and curative effect monitoring marker, belonging to the field of immunodetection. The content of protein (OPN, SAA, CRP, CEA, CYFRA21.1, MIF, AGP, HGF, E-selectin, GRO and NSE) in 11 serums is measured through a Luminex protein chip diagnosis technology. The eight serum protein molecular markers are OPN, SAA, CRP, CYFRA21.1, CEA, NSE, AGP and HGF. The eight serum protein molecular markers have significant promotion effect on a non-small cell lung cancer (NSCLC) and a small cell lung cancer (SCLC). A three-protein detection combination formed by OPN, CEA and another protein (CRP, SAA, CYFRA21.1 or NSE) has excellent diagnosis potential on NSCLC. Compared with the prior art, the serum molecular marker combination has the beneficial effects that the content of multiple protein molecular markers of serum of a patient with lung cancer is detected, and the serum molecular marker combination can be used for lung cancer diagnosis and curative effect monitoring through coordinated comparative analysis of the multiple protein molecular markers.

The invention discloses a visual protein chip for detecting serum antibody of new-castle disease virus of chickens, infectious bronchitis virus of chickens, avian influenza virus and infectious bursal disease virus of chickens , which is prepared by the following steps: purifying and diluting whole proteins of the four virus respectively; pointing samples of the positive control serum, the negative control serum and the four virus proteins onto a chip carrier respectively; drying, fixing, sealing and washing the samples to obtain the visual protein chip. The visual protein chip uses the purified whole proteins as capturing antigens to detect the virus-specific antibodies in chicken serum so as to simplify the preparation technology and reduce the production cost, and the visual protein chip has better specificity but no cross, has high reliability of results and has the advantages of quickness, simplicity and convenience, high sensitivity, good specificity and the like. When the serum is diluted by 6,400 times, the visual protein chip still can detect the antibodies, the sensitivity is 400 times of that of the prior AGP detection method. According to the detection to serum samples in-place, the detection rate of the visual protein chip is higher than the proir AGP method remarkably.

The invention discloses a kit for identifying a new coronavirus by applying a mass spectrum system and a use method of the kit. The kit comprises the following components: a U9 buffer solution, a U1 buffer solution and a sodium acetate buffer solution. The using method of the kit comprises the following steps: S1, activating a metalloprotein chip target plate; S2, pretreating a serum sample; S3, extracting protein in the serum sample by using the metalloprotein chip target plate; and S4, acquiring and processing a spectrogram by using an MALDI-TOF mass spectrum system, and analyzing the read spectrogram by using software. Therefore, the kit is high in sensitivity, good in accuracy, simple and convenient to operate, short in consumed time and very suitable for clinical diagnosis.

The invention belongs to the technical field of biology, and particularly discloses a method for quantitatively detecting a protein acetylation level. The method particularly comprises the steps of preparing protein chips, drawing standard curves and detecting actual samples. The method overcomes the defects that antibodies have weak affinities and acetylated protein is difficult to enrich efficiently when acetylation antibodies are subjected to immune precipitation enriching directly; the protein acetylation level can be quantitatively detected quickly and simply; the used sample amount is less; the difference comparison among the different samples is facilitated; the study of an acetylation difference spectrogram provides a technology platform for establishing high-flux protein acetylation difference spectral analysis; and a predictive value of an acetylation state for liver cancer metastasis can be investigated.

The invention provides a multifunctional fluorescent protein nanowire. The multifunctional fluorescent protein nanowire comprises a protein nanowire and fluorescent molecules connected to the outer side of the protein nanowire, wherein the protein nanowire is formed through self-assembly of wire forming protein. According to the multifunctional fluorescent protein nanowire, fluorescent molecules serve as signal molecules, the surfaces of the fluorescent molecules at the two ends of the multifunctional fluorescent protein nanowire are modified with function ligands to serve as binding molecules, and the multifunctional fluorescent protein nanowire can be used for improving the sensitivity of immunodetection, especially protein chip detection.

The invention relates to a recombinant rubella virus E1 protein and an application thereof. The adopted technical proposal is as follows: the amino acid sequence of the recombinant rubella virus E1 is shown in SEQ ID NO: 2, a reagent kit which is prepared by the recombinant rubella virus E1 protein and used for detecting the rubella virus IgM antibody infection is adopted, compared with the similar reagent kits on the market, the reagent kit has the advantages of strong specificity, high sensitivity, and the like, thereby well meeting the needs of clinical diagnosis of rubella virus infection. The invention further provides the application of the recombinant rubella virus E1 protein in the preparation of monoclonal antibodies, polyclonal antibodies and protein chips.

This invention relates to one functional central neutral damage dialogue test protein chip and its process method, which comprises the following steps: using protein chip to fix multiple central neutral system damage generation development key function abnormal protein factor antigen and its array; the said protein antigen and its array abnormal protein factors act as key property protein factors in the neutral system for inflammation and activation reaction and cell ion balance adjust and signal transmission.

The present invention discloses one kind of gene recombinant human cytomegalovirus fusion protein pp150 / MDBP and its preparation process and application, and relates to gene engineering technology, preventing vaccine, diagnosis reagent and other technology. The recombinant fusion protein pp150 / MDBP is fusion protein formed through connecting serially the 197th amino acid in the 495-691 amino acid segment of human cytomegalovirus pp150 and the 64th amino acid in the 538-601 amino acid segment of MDBP protein. The 197th amino acid pp150 in the N-terminal of the fusion protein and the 64th amino acid of MDBP protein in the C-terminal of the fusion protein are connected through two amino acids including one Leu and one Glu, and the fusion protein has one increased Met and has whole length of 264 amino acids. The fusion protein is used in detecting human cytomegalovirus antibody and antigen, preparing antibody and protein chip, vaccine, ELISA detecting kit and other products.

The invention provides a protein chip for typing detection on helicobacter pylori infection. The protein chip comprises a basement membrane and antigens which are respectively dotted on the basement membrane in the form of dot matrix, wherein the antigens include three types, i.e. CagA (cytotoxin-associated gene) antigens, VacA (Vacuolating cytotoxin A) antigens and Ure (ureaplasma urealyticum) antigens. Due to the fact that the detection antigens used by the protein chip are three single proteins, i.e. Ure, CagA and VacA, specific IgG antibodies for the three antigens in the blood serum of a patient can be detected at the same time, I-type infection or II-type infection can be judged, and clinic treatment is better facilitated; furthermore, the protein chip has the advantages of high detection sensitivity, quick, simple and convenient detection, instant readability, small using amount of antigen and the like.

The invention relates to the field of biotechnical detection, and in particular relates to a protein chip for detecting syphilis as well as a preparation method and application thereof. A surface dot matrix of a solid-phase vector of the protein chip is fixed with a treponema pallidum recombinant antigen rTpN15-17-47; and the protein chip disclosed by the invention can be used for respectively detecting IgG and IgM antibodies in blood serums of patients suffering syphilis, is high in sensitivity, ensures that the visual detection limit is as low as 0.19mu g / mL and the positive rate can reach 99.0%, and ensures that both the visual detection limit and the positive rate are higher than those of conventional TRUST and TPPA detection methods.

The invention discloses a method for seeking micromolecule chemical drug target spots by combining a quantum dot nanometer fluorescent probe and a protein chip, comprising the steps of: connecting a quantum dot with a micromolecule chemical drug to form a quantum dot-micromolecule chemical drug fluorescent probe; then directly incubating the fluorescent probe and the protein chip together; after washing, determining the binding sites (target spots) of specificities between the drug and known proteins fixed on the chip according to fluorescence given out by the optical excitation of the quantum dot on the protein chip; and analyzing the proteins at the binding sites through computer software. According to the method for seeking micromolecule chemical drug target spots by combining the quantum dot nanometer fluorescent probe and the protein chip, the micromolecule chemical drug and the target proteins having efficacy on cells can be simply, conveniently and rapidly sought, and an efficient technical means for screening novel micromolecule chemical drugs in a high-throughput manner can be provided.

The invention discloses a dual fluorescent microsphere immunological detection method for pseudorabies virus gE and gB IgG antibodies. The detection method is based on a liquid protein chip technology-based carboxylated fluorescent microsphere group for detecting pseudorabies virus antibodies; the group comprises carboxylated fluorescent microspheres coupled with gE truncated proteins and gB truncated proteins, respectively; and the amino acid sequences of gE and gB truncated proteins are shown in SEQ ID NOs: 2 and 4, respectively. The dual fluorescent microsphere immunological detection method for simultaneously detecting PRV gE and gB IgG antibodies is good in reproducibility, high in sensitivity and good in specificity, and has no cross-reaction with other common porcine virus-positiveserums. The method can be used for the rapid differential diagnosis of pseudorabies virus wild-type infected pigs and vaccine-vaccinated pigs and the detection of protective antibodies, thereby providing an important method for the monitoring of swine virus diseases, having a great application value and being worthy of large-scale promotion.

The invention belongs to the field of biomedicine, providing a method for detecting animal pathogenic microorganisms and a special protein chip thereof. The detecting method essentially comprises the following steps of: a. preparing the protein chip, selecting a plurality of different groups of specific antigen of many kinds of pathogenic microorganisms by means of an experiment to be fixed on a properly modified carrier; and b. detecting: reacting blood serum of a animal to be detected with the protein chip, hybridizing with a marked secondary antibody, detecting a hybrid positive signal in a proper detection way, and obtaining a hybrid result of the blood serum and the chip by means of scanning and analyzing, wherein the blood serum infected with the different pathogenic microorganisms can show different positive signal combination modes after the reaction, so as to ensure whether the animal to be detected is infecting or is infected with the pathogenic microorganisms marked on the protein chip. The invention can simultaneously detect many kinds of the pathogenic microorganisms, improves detecting efficiency, selects many groups of different antigen with a certain specificity aiming at each pathogenic microorganism, leads the detection to have good specificity, and has good application prospect in detecting the animal pathogenic microorganisms.

The invention discloses a combined general check protein chip for early-stage cancers mainly comprising lung cancer and belongs to the technical field of a protein chip. The combined general check protein chip for the early-stage cancers mainly comprising the lung cancer, provided by the invention, comprises a chip substrate and marker antibodies distributed on the chip substrate, wherein the chip substrate is an FAST protein chip substrate having a three-dimensional nitrocellulose basic material; one or more sample application module is arranged on the FAST protein chip substrate; the following marker antibodies are fixed on the sample application module: 14-3-3 theta, LAMR-1, TSGF (Tumor Specific Growth Factor), CEA (Cancer Embryo Antigen), SCC (Squamous Cell Carcinoma), CYFRA21-1 and positive control; the following marker antibodies are also fixed: AFP (Alpha Fetal Protein), CA242, MG-Ag, CA199, TPA (Tissue Polypeptide Antigen) and EA-IgA; and the following marker antibodies are further fixed: CA125, CA153, SF (Serum Ferritin) and PSA (Prostate Specific Antigen). According to the combined general check protein chip provided by the invention, the related indexes are highly integrated; the combined general check protein chip has the advantages of excellent stability, strong specificity, long retention period, high sensitivity, excellent repeatability, convenience in operation and low cost; the labor efficiency is increased; and a cancer patient is diagnosed and treated from the general check before clinical symptoms occur.

The invention belongs to the field of molecular biology and oncology, and particularly relates to a group of esophageal cancer detection markers and application thereof in preparation of an esophagealcancer screening kit. The esophageal cancer detection markers serve a reagent capable of specifically detecting CA19-9, CYFRA21-1, CA125, FBXW7 and FAT1 or a group of gene expression products; the esophageal cancer early diagnosis kit is a liquid-phase protein chip kit. According to the esophageal cancer marker, a combination of the CA19-9 monoclonal antibody, the CYFRA21-1 monoclonal antibody, the CA125 monoclonal antibody, the FBXW7 monoclonal antibody and the FAT1 monoclonal antibody is used as the esophageal cancer marker for the first time, and is applied to preparation of a liquid-phaseprotein chip with high detection sensitivity and specificity for early esophageal cancer, the detection sensitivity of the liquid-phase protein chip to esophageal cancer is 93.5%, and the specificityof the liquid-phase protein chip to esophageal cancer is 73.9%.

The invention discloses an upconversion-based luminous marker, a protein chip and a detection method. An antibody A is immobilized at a detection site of a bioactive molecule array on a glass slide or other carrying medium and can be specifically bound with an immune complex target antigen, and a quality testing antibody is immobilized at a quality testing site of the bioactive molecule array and can be specifically bound with a quality testing substance antigen in a pretreatment solution; an excitation device for line scanning is a near infrared light source which irradiates the protein chip through a dichroscope, a detection device used in a linear scanning technology is a line array camera, and the line array camera collects visible light intensity signals sent from each point on the line. UCNPs (upconversion nanoparticles) are crosslinked with bioactive molecules in a covalence manner, and the reliability and the stability of a system are improved on the premise that the detection sensitivity is guaranteed.

The invention discloses a protein chip for combined detection of multiple thrombus markers. A preparation method of the protein chip comprises the following steps: (1) black slide pretreatment; (2) antibody solution sample application; (3) a sealing process; and preparation of the protein chip. The protein chip is used for detecting concentrations of various thrombus markers, preventing and reducing thrombus risks and guiding thrombus treatment. The product disclosed by the invention is a protein chip diagnostic kit comprising six indexes of TAT (thrombin-antithrombin complex), PIC (plasmin-alpha2 anti-plasminogen complex), tPAIC (tissue-type plasminogen activator-plasminogen activation inhibition complex), TM (thrombomodulin), FDP (fibrinogen degradation product) and DD (D-dimer). The indexes are detected by using a protein chip technology for the first time, and the method has the advantages of quickness, high efficiency, simplicity, convenience, low cost and the like.

The invention relates to the technical field of biology, in particular to a preparation method an application of a protein chip for detecting pet infection markers. The preparation method of the protein chip comprises the following steps: (1) carrying out pretreatment on a glass slide; (2) performing sample application on an antibody solution; (3) preparing the protein chip by using a sealing process. The protein chip provided by the invention is used for the dog and cat infection markers; after the protein chip technology originated by the company is applied to realization of joint detection,the detection efficiency is effectively improved, and the detection cost is lowered; the inventor develops a protein chip diagnostic kit capable of realizing canine C-reactive protein (CRP) / serum amyloid A (SAA) double-test, canine CRP / SAA / CPL triple-test, feline CRP / SAA double-test and feline CRP / SAA / FPL triple-test; the preparation method has the advantages of being fast, high in efficiency, low in cost, and the like.

The invention belongs to the technical field of biological medicines and particularly relates to a biological marker group for optic neuromyelitis spectrum diseases, application of the biological marker group, a protein chip and a kit. The invention provides an optic neuromyelitis spectrum disease biomarker group which is characterized by comprising monocyte chemotactic protein-3, LIGHT, macrophage inflammatory protein-1 delta, insulin-like growth factor-2, a glucocorticoid induced tumor necrosis factor receptor, thrombopoietin and a herpes virus entry medium, wherein the LIGHT is a lymphotoxin analogue which can be induced and expressed on a T cell and competes with glycoprotein D of HSV to be combined with HVEM. The application fills the blank that no reliable and accurate product and method for diagnosing and identifying optic neuromyelitis lineage diseases exist clinically at present.

The invention discloses a protein combination for diagnosing a Q fever. The protein combination consists of the following proteins from (1) to (7): (1) a protein of which the amino acid sequence is represented by SEQ ID NO: 1, (2) a protein of which the amino acid sequence is represented by SEQ ID NO: 2, (3) a protein of which the amino acid sequence is represented by SEQ ID NO: 3, (4) a protein of which the amino acid sequence is represented by SEQ ID NO: 4, (5) a protein of which the amino acid sequence is represented by SEQ ID NO: 5, (6) a protein of which the amino acid sequence is represented by SEQ ID NO: 6, and (7) a protein of which the amino acid sequence is represented by SEQ ID NO: 7. The protein combination and a protein chip can perform quick and accurate serology analysis and diagnosis on blood serum of a human or an animal having the Q fever; the result is accurate and reliable; moreover, the operation is simple, the number of used samples is low, the time and the labor are saved, and the protein combination is desired to be substituted for the conventional complicated, tiring and time-consumed serology diagnosis method for Q fever serology diagnosis.

The invention discloses a Hepatitis C virus variation detection protein chip as well as a preparation method and application thereof. The invention mainly adopts colloid gold-silver dye detection system to replace original fluorescent marker of the chip, with the application of the detection system of the invention, a chip fluorescent scanner is unnecessary, only by visual detection and simple image scanning can the reaction result of the chip be judged, thus realizing the aim of HCV variation detection. Compared with the fluorescent detection system, the invention is more suitable for fundamental medical treatment organs, can be applied to prognosis of HCV diseases, prediction of curing effect of interferon and the like, and has certain clinical application prospect.

The invention belongs to the technical field of medical biology, and particularly discloses a biomarker for auxiliary diagnosis of esophageal squamous carcinoma. The biomarker for diagnosing esophageal squamous cell carcinoma, provided by the invention, is at least one of autoantibodies of anti-tumor related antigens MAGEA1, VCL, TP53 and PRKCZ. The invention also provides a kit for auxiliary diagnosis of esophageal squamous carcinoma, the kit contains a reagent for detecting the biomarker, and the reagent is used for detecting the biomarker in a sample through enzyme-linked immunosorbent assay, a protein chip, immunoblotting or microfluidic immunoassay. By detecting the expression levels of autoantibodies of anti-tumor related antigens MAGEA1, VCL, TP53 and PRKCZ in human serum, esophageal squamous cell carcinoma patients and normal people can be effectively distinguished, and the kit can be used for auxiliary diagnosis of esophageal squamous cell carcinoma.

The invention provides a construction method of a novel coronavirus N-His recombinant protein chip platform. The construction method comprises the following steps: firstly, preparing N recombinant protein with good antigenicity through pET28a N plasmids; and then determining the optimal dilution of the first antibody and the second antibody by using the N recombinant protein, and finally determining the detection limit and the linear range of the chip by using the constructed chip to detect the serum subjected to gradient dilution, thereby completing the construction of the N gene recombinantprotein chip platform. Compared with an RT-PCR detection method, the chip has more stable detection performance, also has the characteristics of high throughput, microminiaturization and the like, isnot easily influenced by virus variation, and can more accurately and quickly realize large-batch screening of 2019-nCoV.