Preparation method and application of single cell or tissue particle suspension

Abstract

The invention relates to a single cell or tissue particle suspension, belonging to the technical field of biomedicine. The suspension is prepared by the following steps: 1) tissue sample is collected; 2) single cell or tissue particle buffer solution is prepared; 3) the single cell or tissue particle buffer solution is washed; 4) the single cell or tissue particle suspension is prepared. In the invention, multiple single cells or tissue particles which have positive control significance or single cell or tissue particle which has multiple positive control significances are combined, control can be formed while only a drop of the suspension is dropped, and no slicing is required, thus detection procedure is simplified; the suspension can be applied to in-situ hybridization, FISH technologyand dyeing of pathogens, thus having wide application range; the single cell or tissue particle suspension is convenient to store, is easy to be stored for a long time and can be repeatedly used; anddetection result is ensured to be accurate, thus being convenient for popularization and being helpful to comprehensively and easily solve the problem of quality control of in situ detection in immunohistochemistry.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of a single cell or tissue microparticle suspension which is easy to use and has high detection method accuracy. Background technique [0002] Immunohistochemistry, in situ hybridization and fluorescence in situ hybridization are more and more widely used in pathological work, for the determination of the benign and malignant of some lesions and the nature of tissue cells, as well as for radiotherapy, chemotherapy, anti-hormonal therapy, and newly developed target It plays an important role in providing indicators for treatment and neoadjuvant chemotherapy. However, there are many factors affecting its quality, and the original positive control method is troublesome for many lesions that lack their own positive control. The judgment of the result is not guaranteed, resulting in inappropriate diagnosis and treatment, and the res...

AI Technical Summary

Problems solved by technology

However, the methods introduced are cumbersome to operate; the mutual friction of spare slices makes it difficult to transport and store, and there is no guarantee for re-use for sectioning; the workload increases greatly, the volume of the control object is large, and the consumption of reagents is doubled.

Due to the above defects, it is difficult for pathological technicians to accept these methods, making these methods unable to be popularized and applied in actual work

The traditional way to establish a control is that a group of test slides can only set up a positive photo as a control by slicing, but neither machine operation nor manual operation can guarantee that the detection conditions of each slide are consistent, such as the time of each step. , the amount of reagents used, the degree of potency or other human factors, etc., all have differences, which will make it difficult to judge the accuracy of some results

At present, it is a common phenomenon that there is no positive control in our country, and it does not meet the quality control requirements.