Research and application of molecular identification-based fluorescence nanocrystal quartz fluorescent sensor for high-selectivity multicomponent saccharide detection

Abstract

The invention discloses a fluorescence nanocrystal quartz fluorescent sensor for molecular identification-based multicomponent simultaneous saccharide detection and a method for detecting saccharides by using the same. The invention provides a preparation method of a molecular identification-based fluorescence nanocrystal comb quartz plate, which comprises the following steps: selecting an identifier corresponding to the saccharides; preparing a fluorescence nanocrystal, and carrying out surface modification on the fluorescence nanocrystal according to the literature; and modifying the modified fluorescence nanocrystal onto surfaces of different probes of the comb quartz plate by using layer-by-layer accumulation surface modification technique. The invention also provides a method for multicomponent simultaneous saccharide detection, which comprises the following steps: immersing the modified quartz plate into a food sample solution which is simply pulpified, installing the quartz plate onto a sealed silica dish, and detecting the saccharides in the sample. The invention has the advantages of strong specificity, high sensitivity, short detection time and low cost. The saccharide fluorescence detection method has a quick and simple operation process, and the reaction and the result are automatically completed and recorded by instruments.

Description

technical field [0001] The present invention relates to the technical field of on-site rapid and highly selective sugar detection, and more specifically to the preparation of a molecular recognition fluorescent nanocrystal quartz fluorescent sensor for detecting sugars in a sample. The present invention also relates to the use of the molecular recognition fluorescent nanocrystal A method for the on-site detection of sugars in vegetable, fruit and food samples with crystal quartz fluorescent sensors. Background technique [0002] Sugar is the most abundant and widely distributed organic compound with important biological functions in nature. Sucrose in daily consumption, starch in food, cellulose in plants, glucose in human blood, etc. are all sugars, and the organisms from bacteria to higher animals contain sugar compounds. The content is most abundant in plants, while other organisms use glucose, starch and other sugars as nutrients, absorb them from food and transform the...

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Problems solved by technology

[0005] 2. Causing calcium deficiency The disadvantage of white sugar is that when it turns into glucose, it consumes a lot of vitamin B and calcium. If you eat too much sugar and various sweets, it is easy to cause vitamin B1 and calcium deficiency

[0006] 3. Affect the healthy growth of children, especially unfavorable to the growth and development of teenagers

[0010] 7. Affects bone development Sugar is an acidic substance that does not contain calcium. Often eating sugar, sweets, chocolate or sugary drinks will consume a lot of calcium, which will not only affect bone development, but also easily lead to fractures

[0016] 3. For the detection and analysis of sugars, the above methods generally have disadvantages such as high detection cost, complicated detection process, inaccurate results, and not suitable for rapid on-site detection, so they cannot meet the needs of actual detection.

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