Kit for detecting aldehyde dehydrogenase 2 gene polymorphism and amplification method and detection method thereof

A technology of gene polymorphism and acetaldehyde dehydrogenase, which is applied in the field of biomedicine, can solve the problems of reduced activity of acetaldehyde dehydrogenase enzyme 2, reduced ability of alcohol metabolism and detoxification, and reduced curative effect of nitroglycerin, so as to reduce operation steps , avoid human error, avoid the effect of false negative results

Inactive Publication Date: 2013-07-03
UNION STEMCELL & GENE ENG +1
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Problems solved by technology

[0003] A number of studies have shown that the reduction of aldehyde dehydrogenase enzyme 2 activity has an impact on the reduction of the curative effect of nitroglycerin, the reduction of alcohol metabolism and detoxification ability, and the occurrence of various diseases
Sublingual administration of nitroglycerin tablets is the first choice for routine anti-angina pectoris, but the clinical effectiveness of the summary varies from person to person, and some patients have no effect after taking it. Among the Chinese Han population, the proportion of nitroglycerin ineffective Up to 25%, much higher than the European and American population

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  • Kit for detecting aldehyde dehydrogenase 2 gene polymorphism and amplification method and detection method thereof
  • Kit for detecting aldehyde dehydrogenase 2 gene polymorphism and amplification method and detection method thereof
  • Kit for detecting aldehyde dehydrogenase 2 gene polymorphism and amplification method and detection method thereof

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[0029] The kit for detecting the polymorphism of the aldehyde dehydrogenase 2 gene of the present invention, the kit can perform amplification and typing detection on the rs671 SNP site of the ALDH2 gene, and use the amplification product of the external primer as an internal reference to detect the amplification condition; 2 × buffer the wild-type and mutant sequence-specific primers containing the SNP site, the upstream and downstream primers of the outer sequence, and determine whether the genotype phenotype of the SNP site is carried by the presence or absence of DNA electrophoresis bands after amplification; when the wild-type The band corresponding to the fragment length is positive in the amplification. When the band at the corresponding position of the mutant type is negative, it is interpreted as the wild homozygous type. On the contrary, it is the mutant homozygous type. Both the wild type and the mutant type corresponding to the fragment length position appear The re...

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Abstract

The present invention discloses a kit for detecting aldehyde dehydrogenase 2(ALDH2) gene polymorphism by using the single-tube bidirectional allele-specific amplification technology combined with the SNP sensitivity molecular switch technology, and an amplification method and a detection method thereof. The kit genotypes the Glu487Lys(rs671) site on the aldehyde dehydrogenase 2. The kit includes amplification buffer, polymerase, cell lysis buffer and glycerol, and can complete the genotyping for the SNP site in one PCR reaction so as to understand the genotype of the aldehyde dehydrogenase 2 subject and the product activity thereof.

Description

technical field [0001] The invention relates to a kit for detecting the polymorphism of acetaldehyde dehydrogenase 2 gene by using single-tube bidirectional allele-specific amplification technology combined with molecular switch technology and its amplification method and detection method, in particular to a method for detecting ALDH2 A reagent kit and a PCR amplification method for a single nucleotide polymorphism (SNP) at a gene rs671 site belong to the field of biomedicine. Background technique [0002] The human ALDH2 gene is located on chromosome 12 and contains 13 exons. When the guanine (G) at position 1510 of exon 12 is mutated to adenine (A), the glutamic acid at position 487 of the protein encoded by the gene ( Glu) was replaced by lysine (Lys), namely Glu487Lys(rs671). This mutation can cause a significant decrease in ALDH2 enzyme activity and weaken the biological function of ALDH2. [0003] A number of studies have shown that the reduction of aldehyde dehydrog...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 韩俊领杜宏伟周毓玲崔丽娟
Owner UNION STEMCELL & GENE ENG
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