Nucleic acid detection kit for six respiratory viruses and use method

Abstract

The invention discloses a nucleic acid detection kit for six respiratory viruses and a use method. The nucleic acid detection kit for the six respiratory viruses comprises six respiratory virus reaction liquid, positive control liquid and negative control liquid, and the six respiratory virus reaction liquid is freeze-dried powder which is pre-sub-packaged into eight connecting tubes, wherein the six respiratory virus reaction liquid A / B tubes contain a plurality of probes with different fluorescence labels, primers, enzyme mixtures, reinforcing agents, freeze-drying protective agents, 10 * buffer, nucleotide mixtures and the like; whether respiratory syncytial virus, respiratory adenovirus, human metapneumovirus, parainfluenza virus type I, parainfluenza virus type II and parainfluenza virus type III exist or not can be detected in the same reaction system, each of the A / B tubes is provided with an internal reference control, and the internal reference control is a human conserved gene beta-actin fragment marked as ROX; and the internal reference control can display the occurrence of false negative, so that the occurrence of a false negative interpretation result caused by an inhibitor in a sample or misoperation is avoided, and the accuracy of PCR detection is improved.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection of six respiratory viruses, in particular to a nucleic acid detection kit of six respiratory viruses and a use method thereof. Background technique [0002] At present, respiratory tract infection is a common clinical disease, which can be caused by various viruses. Common respiratory viruses include: respiratory syncytial virus, respiratory adenovirus, human metapneumovirus, parainfluenza virus, influenza A virus, influenza B virus, etc. Respiratory virus infection can cause a variety of diseases, such as cold, flu, pharyngitis, laryngitis, bronchitis, pneumonia, etc. The main clinical symptoms after human infection include fever, runny nose, sore throat, cough, headache and diarrhea. Because the symptoms of infection caused by these viruses are similar to those of influenza, it is difficult to distinguish the pathogen by clinical symptoms alone. Laboratory diagnostic methods incl...

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Problems solved by technology

[0003] The invention provides a six-item respiratory virus nucleic acid detection kit and its use method, which solves the problem that it is difficult to simultaneously detect multiple viruses and effectively control the detection process in existing respiratory virus nucleic acid detection

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