Kit for detecting alcoholic liver disease susceptibility

A technology of alcoholic liver disease and kit, applied in the direction of microbiological determination/testing, biochemical equipment and methods, etc., can solve problems such as bad living habits and changes, and achieve the effects of high sensitivity, good repeatability, and high accuracy

Inactive Publication Date: 2011-08-10
UNION STEMCELL & GENE ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is, by detecting a group of genes and loci related to the susceptibility of alcoholic liver disease, using specific primers and probes, combining single nucleotide extension technology with microarray chip techn

Method used

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  • Kit for detecting alcoholic liver disease susceptibility
  • Kit for detecting alcoholic liver disease susceptibility
  • Kit for detecting alcoholic liver disease susceptibility

Examples

Experimental program
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Embodiment Construction

[0043] The present invention will be described in further detail below in conjunction with specific embodiments. For the scale-up of experiments without specific conditions indicated in the following examples, conventional conditions or the conditions suggested by the manufacturer are generally followed.

[0044] 1 DNA extraction:

[0045] Take the peripheral venous blood of the subject, add the same volume of cell lysate, and lyse twice to fully lyse the white blood cells. After centrifuging and discarding the supernatant, add proteinase K buffer and proteinase K (50ug / ml), incubate at 65°C for 10 minutes, iso Precipitate with propanol, wash twice with 75% ethanol, dry and dissolve in appropriate amount of TB solution.

[0046] 2PCR amplification

[0047] Take the subject's genomic DNA extract and add it to a 96-well plate for multiplex PCR amplification:

[0048]The total reaction volume is 5ul, of which 10M mol / L dNTPs 0.0375ul, 10×PCR Buffer0.5ul, 25mmol / L MgCl 2 1ul, ...

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Abstract

The invention discloses a kit for detecting alcoholic liver disease susceptibility. The kit detects three genes closely related with an alcoholic liver disease, namely an ADH2 gene of alcohol dehydrogenase, an ALDH2 gene of acetaldehyde dehydrogenase and a CYP2E1 gene of cytochrome P4502E1. The simultaneously detected SNP sites comprise an rs1229984 site of the ADH2 gene, an rs671 site of the ALDH2 and an rs2031920 site of the CYP2E1. Whether a detected crowd carries ''alcoholic liver disease susceptible genes'' can be clearly judged by using the kit, detecting a group of genes and sites related with the alcoholic liver disease susceptibility, using specific primers and probes and combining mononucleotide extension technology and micro array chip technology, and the alcoholic liver disease susceptible crowd is screened from the crowd, so that poor life habits are changed and the purpose of prevention is achieved.

Description

technical field [0001] The present invention relates to a kit for detecting susceptibility to disease, especially a kit for detecting susceptibility to alcoholic liver disease. Predicting an individual's susceptibility to leukemia. Background technique [0002] Alcoholic liver disease (ALD) is liver damage caused by long-term drinking, which can be manifested in three forms: alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis. These three forms may exist alone or in admixture. According to statistics, there are about 15 million to 20 million people in the world who are alcoholics, and about 10% to 20% (1.5 million to 4 million) of them have alcoholic liver disease in varying degrees. In my country, with the continuous improvement of people's living standards and economic conditions, the incidence of ALD has also been increasing year by year in recent years. Alcohol has become the second leading cause of liver damage after viruses. [0003] After drinking, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 韩俊领刘志霈周毓玲杜宏伟李楠靳霞刘蓉华
Owner UNION STEMCELL & GENE ENG
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