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Reagent kit for detecting alcoholism and alcohol addiction susceptibility

A technology for alcoholism and alcohol addiction, which is applied in the field of kits and can solve problems such as discomfort, decreased ability to metabolize acetaldehyde, and alcoholism

Inactive Publication Date: 2008-01-23
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein encoded by the low-frequency allele type A has low activity, and the ability to metabolize acetaldehyde is reduced, causing discomfort in drinking, flushing syndrome, etc., and easily leading to alcoholism

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Use of detection kits

[0023] Step 1: Extraction of DNA template

[0024] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0025] Step 2: Real-time quantitative PCR reaction

[0026] Use a real-time quantitative PCR detection kit for detecting susceptibility to alcoholism and alcohol addiction, which contains the following primer pairs and fluorescent probes:

[0027] Sense primer: 5'-TTTGGTGGCTACAAGATGT-3' (SEQ ID NO: 2) with a Tm value of 54°C

[0028] Antisense primer: 5'-CTCCGAGCCACCAGCA-3' (SEQ ID NO: 3) with a Tm value of 54°C

[0029] Fluorescent probe: 5'-GCTGCAGGCATACACTAAAGT-3' (SEQ ID NO: 4) with a Tm value of 62°C

[0030] The fluorescent quantitative PCR reaction system has a total volume of 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, 0.1 μl of 25mM dNTP mixture, and 0.5 μl of 25mM MgCl 2 solution, 0.02 μl (5 units / μl) Taq DNA...

Embodiment 2

[0034] Example 2. Services to Instruct People to Proactively Prevent Alcoholism and Alcohol Addiction

[0035] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0036] Step 1: DNA Extraction

[0037] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the drinking habits questionnaire. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0038] Step 2: Genotyping Assays

[0039] Using the kit provided by the present invention, the rs671 SNP site on the ALDH2 gene of the tested subject's DNA is detected by fluorescent quantitative PCR, and the genotype of the SNP site is determined.

[0040] Step 3: Instruct people to proactively prevent alcoholism and addiction

[0041]Through the analysis of the SNP genotype of the tested person, the genotyping test ...

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PUM

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Abstract

The invention discloses a reagent box for detecting alcoholic intoxication and susceptibility to alcohol habituation. The reagent box comprises specificity primer pairs and specificity fluorescent probes for detecting position 161 SNP position in ALDH2 gene SEQ ID NO:1, and an ordinary assembly for fluorescent quantitative PCR detection. The reagent box predicts the alcoholic intoxication and susceptibility to alcohol habituation of an individual by detecting and analyzing the gene carried on position SNP on ALDH2 gene.

Description

technical field [0001] The invention relates to a kit for detecting susceptibility to diseases, in particular to a kit for detecting susceptibility to alcoholism and alcohol addiction. Nucleotide polymorphism (SNP) loci to predict individual susceptibility to alcoholism and alcohol addiction. Background technique [0002] Alcohol consumption is one of the important ways of life of human beings. When drinking too much alcohol or alcoholic beverages at one time, the central nervous system will change from excitement to inhibition, which is called alcoholism or ethanolism. [0003] Alcoholism can inhibit the central nervous system, causing drowsiness and coma. Extremely high concentrations of ethanol can inhibit the medullary center and cause respiratory and circulatory failure. In addition, ethanol is metabolized in liver cells to generate a large amount of reduced nicotinamide adenine dinucleotide (NADH), which increases the ratio of NADH / NAD to the oxidized form, which can...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/68G01N33/52G01N33/48C12Q1/68
Inventor 冯哲民邹祖烨楼屹
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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