Detection method of gene related to alcohol metabolism

A detection method and gene technology, applied in the field of detection of genes related to alcohol metabolism, can solve problems such as poor detection accuracy, and achieve the effect of low cost, fast speed and reliable experimental results

Inactive Publication Date: 2017-12-08
安徽安龙基因科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a detection method for ...

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  • Detection method of gene related to alcohol metabolism
  • Detection method of gene related to alcohol metabolism
  • Detection method of gene related to alcohol metabolism

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Experimental program
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Embodiment 1

[0047] refer to Figure 1-5 , Li was tested for genes related to alcohol metabolism

[0048] 1. Extract DNA from Li's oral mucosal cells with oral swab DNA extraction kit, the DNA concentration is 32ng / ul, 260 / 280=1.81.

[0049] 2. Use SEQ NO 1 and SEQ NO 2 to amplify the ADH1B gene rs1229984SNP site, use SEQ NO 3 and SEQ NO 4 to amplify the ALDH2 gene rs671SNP site, and use SEQ NO 5 and SEQ NO 6 to amplify the CYP2E1 gene rs3813867 and rs2031920SNP site, using the extracted DNA as a template for PCR reactions,

[0050] Its 25uL system, which includes:

[0051] Template: 2uL, SEQ NO 1: 1uL, SEQ NO 2: 1uL, 10×MSP buffer: 2.5uL, 20mM dNTP: 2ul, Hot start taq: 0.3ul, deionized water: 16.2uL,

[0052] Template: 2uL, SEQ NO 3: 1uL, SEQ NO4: 1uL, 10×MSP buffer: 2.5uL, 20mM dNTP: 2ul, Hot start taq: 0.3ul, deionized water: 16.2uL,

[0053] Template: 2uL, SEQ NO 5: 1uL, SEQ NO 6: 1uL, 10×MSP buffer: 2.5uL, 20mM dNTP: 2ul, Hot start taq: 0.3ul, deionized water: 16.2uL,

[0054] Th...

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Abstract

The invention discloses a detection method of a gene related to alcohol metabolism. The detection method comprises the specific steps that DNA of a detected person is extracted, and the concentration of the extracted DNA is not lower than 10 ng/ul, 260/280=1.8; SEQ NO 1 and SEQ NO 2 are utilized to amplify the rs1229984SNP locus of an ADH1B gene, SEQ NO 3 and SEQ NO 4 are utilized to amplify the rs671SNP locus of an ALDH2 gene, SEQ NO 5 and SEQ NO 6 are utilized to amplify the rs3813867 and rs2031920 SNP loci of a CYP2E1 gene, and the extracted DNA serves as a template to conduct PCR reaction; the three pairs of primers are subjected to agarose gel electrophoresis which is 1% of a common PCR amplification product; the 1% agorose gel electrophoresis strip is used for recycling a target strip by using an agarose gel extraction kit; sequencing reaction is conducted; a product obtained through the sequencing reaction is purified, denatured, and subjected to sequencing on a 3730 sequenator; and Chromas software is utilized to analyze the four SNP gene types from the sequencing result. The detection method of the gene related to alcohol metabolism has the advantages of being capable of conveniently conducting detection, high in detection accuracy and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection method for genes related to alcohol metabolism. Background technique [0002] China is a big consumer of alcohol, which is a mixture of various chemical components. Ethanol is its main component. After drinking, ethanol quickly enters the blood through the capillaries of the stomach and small intestine, and then transports to the liver for digestion through the blood. Alcoholism is Since ethanol enters the human body and cannot be digested and absorbed, it enters the brain with the blood, destroys the neuron cell membrane, weakens the central nervous system, and is formed by sluggish brain activity by activating inhibitory neurons and inhibiting activating neurons. [0003] Drinking alcohol can lead to various diseases, including chronic gastritis, toxic hepatitis, myocardial hypertrophy, urinary tract stones, irreversible brain damage and alcoholism, mental disord...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2531/113
Inventor 韦玉军李航崔俊生苏军吴远航
Owner 安徽安龙基因科技有限公司
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