Primer, probe, kit and method for detection of human ALDH2 gene polymorphism

A gene polymorphism and kit technology, applied in the field of genetic engineering, can solve the problems of inability to accurately locate gene changes, expensive chips, inability to accurately detect gene polymorphisms in samples, etc., and achieve intuitive and convenient result interpretation and fluorescence analysis. Accurate analysis and the effect of reducing background signal strength
CN106947807AInactive Publication Date: 2017-07-14SHANGHAI TISSUEBANK MEDICAL LAB CO LTD +3
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI TISSUEBANK MEDICAL LAB CO LTD
Publication Date
2017-07-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of gene engineering and discloses a primer and a probe combination for detection of human ALDH2 gene polymorphism, a kit comprising the primer and the probe combination, and a fluorescent PCR method for performing the detection of human ALDH2 gene polymorphism with the primer and the probe combination. The primer, the probe and the kit are based on TaqMan fluorescent PCR technology, and are simple, quick and high-sensitive. In addition, through reasonable combination of primer and probe, interaction between primer and primer, primer and probe and probe and probe can be avoided effectively, thus reducing detection error. The primer, the probe and the kit, when being used for the detection of human ALDH2 gene polymorphism, have high sensitivity and strong specificity, are easy, quick and safe to operate, have simple and direct results, and can be applied directly with a blood sample or a dried blood spot specimen on filter paper as a template.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to primers, probes, kits and methods for detecting human ALDH2 gene polymorphisms. Background technique

[0002] Nitroglycerin is an emergency drug for the prevention and treatment of coronary heart disease and angina. The enzyme promotes calcium ions to enter the sarcoplasmic reticulum and the extracellular space, thereby dilating vascular smooth muscle and relieving the symptoms of cardiac ischemia. The polymorphism of ALDH2*2 (Glu504Lys, rs671) leads to the substitution of glutamic acid at position 504 of the encoded protein by lysine, and the ALDH2 enzyme activity of individuals carrying the mutant allele ALDH2*2 decreases, and the enzyme activity of heterozygotes is only wild type In 10% of individuals, the mutation is homozygous for loss of enzyme activity. Therefore, patients with ALDH2*2 type cannot use nitroglycerin to resist myocardial ischemia. The carrier ...

Claims

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