57 results about "Alcohol tolerance" patented technology

The invention discloses a compound type fuel cell cathode catalyst NGPC / NCNTs and a preparation method thereof, belongs to the technical field of catalyst preparation and aims to develop a cheap and efficient non-precious metal catalyst to solve the problem of high cost of a fuel cell cathode catalyst made of a Pt-based material. An MOF (metal-organic framework) material as a C source of the NGPC / NCNTs is pre-carbonized at a low temperature firstly, a graphitization catalyst and a N source are introduced step by step, two times of corresponding high-temperature heat treatment are performed, and the NGPC / NCNTs are prepared. The active component of the NGPC / NCNTs is N-doped graphitized porous C or CNTs, a pore structure is in a micropore-mesopore compound type, and a continuous conduction framework in three-dimensional space is formed through structural connection of dispersed porous C particles by the CNTs, so that the NGPC / NCNTs have good conductivity and higher mass transfer rate and can show efficient catalytic activity, methyl alcohol tolerance and cycle stability, which are better than those of a commercial Pt / C electrocatalyst, in an alkaline medium.

Embodiments of the present invention provide methods and compositions for microorganisms having increased alcohol tolerance. In certain embodiments, methods for using such microorganisms, and methods for identifying gene or genetic regions responsible for increased alcohol tolerance are contemplated.

The invention discloses a nitrogen-doped graphene / ferrocobalt hydrotalcite-like compound non-precious metal difunctional oxygen-reduction catalyst and a preparing method and application thereof. A bimetallic oxide is used as a precursor, the memory effect of a hydrotalcite-like compound is utilized, oxidized graphene and the ferrocobalt hydrotalcite-like compound are assembled, and then the compound is doped with carbon nitride nanosheets under the reduction condition to obtain a nitrogen-doped graphene / ferrocobalt hydrotalcite-like compound composition. The oxygen-reduction catalyst has high catalytic activity to oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) at the same time under the alkaline condition and has high stability and excellent methyl alcohol tolerance, the cost of adopted raw materials is low, the preparing method is simple, operation is easy, and large-scale production can be achieved conveniently.

The invention discloses a nitrogen and cobalt double-doped porous carbon composite bifunctional oxygen catalyst and a preparation method and application thereof. The preparation method comprises the steps that firstly, under a hydrothermal condition, divalent cobalt ions, melamine and sodium alginate are compounded to obtain melamine / cobalt ion-sodium alginate; and secondly, high-temperature calcination is performed, and then the nitrogen-cobalt double-doped porous carbon bifunctional oxygen catalyst is prepared. The catalyst is made of a porous material, the average hole diameter of the catalyst is 2 nm-8 nm, and the specific area is 148.23 m<2> / g-188.27 m<2> / g. According to the catalyst, cobalt exists in the forms of the Co elementary substance, CoO and Co3O4; and nitrogen atoms doped inthe porous carbon material exist in the forms of pyrrole and pyridine. Due to double doping of the cobalt and nitrogen, the catalyst has high oxygen evolution and oxygen reduction catalytic activity,good stability and excellent methyl alcohol tolerance at the same timeunder alkaline conditions, used raw materials are low in cost, the preparation method is simple, operation is easy, and large-scale production is convenient.

Embodiments of the present invention provide methods and compositions for microorganisms having increased alcohol tolerance. In certain embodiments, methods for using such microorganisms, and methods for identifying gene or genetic regions responsible for increased alcohol tolerance are contemplated.

The present invention relates to E. coli mutants, which have enhanced alcohols tolerance and can be used in production of alcohols through fermentation. The present invention also provides a novel method to prepare the alcohol-tolerant E. coli strains.

The invention discloses a high-performance modified epoxy alicyclic amine curing agent. The high-performance modified epoxy alicyclic amine curing agent has the characteristics of being stable in performance and low in irritability and specifically has the following characteristics: the color is transparent, the temperature tolerance can reach 120-150 DEG C, the shore hardness reaches 80-85D, the adhesive force reaches the class A, the alcohol tolerance can have no change in color for 15 days, and the shearing strength is 12-16 MPa; the high-performance modified epoxy alicyclic amine curing agent can be widely applied to the fields of high-temperature baking type anti-yellowing glass paint, normal-temperature self-drying glass paint, solvent-resistant high-adhesion wine bottle paint, adhesives and transparent anti-yellowing electronic potting. The invention also provides a preparation method of the high-performance modified epoxy alicyclic amine curing agent; the raw material components are scientific and reasonable in ratio; the preparation method is scientific and reasonable; the temperature and the time of reaction in the preparation process can be effectively controlled; the curing agent with moderate viscosity, rate and ratio can be prepared; the self moisture absorption property and brittleness of the alicyclic amine can be greatly improved; the adhesive force, the adhesive performance and the solvent resistance of the alicyclic amine are improved.

The invention belongs to the technical field of bioengineering, and discloses a method for improving the alcohol tolerance of yeast by utilizing lactic acid bacteria. The method includes: adding a lactic acid bacteria exogenously into a yeast culture system for mixed culture for 4h to obtain normal saline with resuspended bacteria and an alcohol content of 8% (v / v), and performing stressing undera 30DEG C condition for 90min; centrifugally washing the bacteria subjected to alcohol stress, then dibbling the bacteria onto a yeast extract peptone glucose (YPD) agar culture medium containing 0.01% chloramphenicol, performing standing and culturing at 30DEG C for 36h, then counting bacterial colonies, and calculating the survival rate. Results show that after alcohol stress, the survival rateof the yeast obtained by mixed culture after lactic acid bacteria is added is 23.64%, the survival rate of yeast obtained after pure culture is 3.87%, and the survival rate is increased by 6.11 times.The method is simple and convenient to operate, and can be used for improving the alcohol resistance of yeast.

A health-care product for protecting liver from alcohol damage and nourishing stomach is prepared from the following raw materials in parts by weight: 5-50 parts of kudzuvine flower, 5-50 parts of kudzuvine root, 5-50 parts of artemisia capillaris, 5-50 parts of pollen, 1-10 parts of semen hoveniae extract, 1-10 parts of purple perilla extract, 1-10 parts of orange peel extract and 1-10 parts of talcum powder. The health-care product keeps the natural activity of all formulation raw materials, thereby achieving a remarkable anti-alcohol effect; while the effects of alcohol are dispelled, visceral organs, such as liver and stomach, are protected from damage caused by drinking; the anti-alcohol capability of the human body is enhanced, and the alcohol tolerance is improved. The product can be not only used in drinking emergency but also taken for a long time, has the effects of protecting the liver and nourishing the stomach, as well as reducing blood fat, blood pressure and blood sugar, losing weight and beautifying, can effectively regulate the navel and stomach functions, strengthen the organic immunity and delaying senescence, and has very good health-care effects on dyspepsia, constipation and the like.

The invention discloses an ethanol-tolerant lactobacillus plantarum and application thereof in fermented food. The lactobacillus plantarum XH-1 is classified and named as Lactobacillus plantarum and is preserved in China General Microbiological Culture Collection Center on August 16, 2019. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservationnumber is CGMCC NO.18390. The alcohol tolerance of the ethanol-tolerant lactobacillus plantarum disclosed by the invention can reach 17% (V / V), the ethanol-tolerant lactobacillus plantarum has the characteristic of high yield of gamma-aminobutyric acid, the capacity of producing gamma-aminobutyric acid in an MRS culture medium reaches 170g / l, and the ethanol-tolerant lactobacillus plantarum is applied to wine and vinegar brewing production. The strain can normally grow and reproduce and produce gamma-aminobutyric acid at high yield in a high-concentration ethanol environment for wine and vinegar brewing, the production process is controllable, and the operation is simple and convenient; the content of lactic acid, ethyl lactate and phenethyl alcohol in the finished wine and vinegar is increased, and the sensory quality of the finished wine and vinegar can be greatly improved.

The invention discloses a kit for detecting acetaldehyde dehydrogenase 2 (ALDH2) gene polymorphism by a pyro-sequencing method and a method. By the kit, the ALDH2 gene polymorphism, particularly RS671 single nucleotide polymorphism can be detected. The kit comprises primers which are shown as SEQ ID NO.2-4. By the kit, the accurate, quick and high-throughout detection of ALDH2 gene polymorphism can be realized, so that the alcohol tolerance can be effectively predicted and prevented, and the clinical individual administration of nitroglycerin can be guided.

The present invention relates to compositions and methods for determining whether an individual is predisposed to or protected from alcoholism. In particular, the present invention provides genetic markers useful for the diagnosis, characterization, and treatment or prevention of alcohol tolerance, abuse and / or dependence.

The invention provides a yeast strain kept under CGMCC NO: 15430 and named as Torulaspora delbrueckii. Experimental results show that the yeast strain has good alcohol tolerance, SO2 tolerance and high sugar tolerance, has a fermenting function in a fermenting broth having a sugar concentration of 200-400 g / L, and features low production of volatile acids and high production of glycerol; in brewedsweet white wine and ice wine, the yeast strain can be mixed with Saccharomyces cerevisiae and fermented to reduce the yield of fruit wine volatile acids, improve the content of fruit wine glycerol,and impart more flower aroma to fruit wines. The yeast strain provided herein has high fermenting capacity and good fermenting performance, and has important value in the application to improve fruitwine quality.

The invention provides a gene detection reagent kit for evaluating alcohol tolerance. The gene detection reagent kit comprises six amplification primers for three genes including an internal reference gene GAPDH (glyceraldehyde phosphate dehydrogenase), a gene ADH1B (ethanol dehydrogenase 1B) and a gene ALDH2 (acetaldehyde dehydrogenase 2) and five Taq-man probe primers. One of the Taq-man probe primers is positioned at the internal reference gene GAPDH and is used for carrying out FAM fluorescence labeling; two other Taq-man probe primers are SNP (single nucleotide polymorphism) probes positioned at the gene ADH1B and are used for carrying out VIC and ROX (roxithromycin) labeling; the remaining two Taq-man probe primers are SNP probes positioned at the gene ALDH2 and are used for Cy5 and Quasar705 labeling, BHQ is used as a quenching group, and delta ct values can be computed. The gene detection reagent kit has the advantages that the alcohol tolerance of healthy persons can be evaluated by the gene detection reagent kit from the aspect of genes, and accordingly the healthy persons can know the strength and the weakness of alcohol tolerance, can drink alcohol scientifically and can carry on occasion such as party, gathering and banquet related to alcohol culture.

The invention discloses a cable for nuclear magnetic resonance instruments, and belongs to the technical field of special wires and cables for medical instruments. The cable comprises a cable core, aninner braided layer, an outer braided layer, an isolation layer and a sheath layer. The inner braided layer, the outer braided layer, the isolation layer and the sheath layer are sequentially arranged outside the cable core, the cable core comprises a shielding layer, control lines and power lines, the control lines and the power lines are positioned in the shielding layer, the inner braided layer is positioned outside the shielding layer, tin-plated copper wires are braided to form the inner braided layer, tin-plated copper wires are braided to form the outer braided layer, the isolation layer is made of isolation paper, and the sheath layer is made of polyurethane and is extruded and coated outside the isolation layer by means of armor extrusion. The cable has the advantages that the cable is excellent in dielectric ability, the outer diameter of the cable core can be reduced, and the stability of dielectric constants and the excellent signal transmission capacity can be guaranteed;the cable is flexible and is excellent in electromagnetic interference resistance; the cable is high in strength and elastic modulus, is excellent in low-temperature resistance and alcohol tolerance,is excellent in resistance to chemical corrosion of ETO (ethylene oxide) and the like and is perfect in ultraviolet radiation resistance.

The invention relates to the field of microbial fermentation, in particular to a saccharomyces cerevisiae strain, a method for screening saccharomyces cerevisiae and a process for brewing myrica rubra wine by the aid of the saccharomyces cerevisiae strain. The technical scheme includes that the saccharomyces cerevisiae strain is mature, myrica rubra which falls off from myrica rubra trees to the ground is naturally fermented and screened to obtain the saccharomyces cerevisiae strain, the saccharomyces cerevisiae strain is preserved in the China Center for Type Culture Collection on January 8, 2015, and a preservation number of the saccharomyces cerevisiae strain is CCTCC NO:M2015021. According to the technical scheme, the saccharomyces cerevisiae strain, the method for screening the saccharomyces cerevisiae and the process for brewing themyrica rubra wine by the aid of the saccharomyces cerevisiae strain have the advantages that the excellent high-adaptability saccharomyces cerevisiae strain is high in specificity, fermentation starting speed, fermentation activity, alcohol tolerance, SO2 resistance and alcohol conversion rate and has unique flavor, substances with peculiar smell can be prevented, and the myrica rubra wine brewed by the aid of the process has excellent taste.

The invention discloses an accessory formula used for producing fruit beer with various nutrient elements. The formula comprises the following components by weight: 0.4kg of red beer pigment, 3kg of fruit beer pigment, 30kg of pineapple condensed juice, 10kg of pineapple essence, 0.5kg of foam stabilizer, 18kg of citric acid, 2kg of vitamin C, 20kg of strawberry juice, 2kg of strawberry essence, 1200kg of sugar, 4kg of sodium cyclamate and 3000kg of zymotic fluid. The combined formula can be added into 22KI of beer for producing the fruit beer with various nutrient elements. The produced fruit beer has various fruit fragrances and nutrient elements of fruit juice beverages, has low alcoholic strength and mellow taste, is fragrant, sweet and tasty, also has certain weight-reducing and face-beautifying effects and is very suitable for females, children, the aged and persons with low alcohol tolerance to drink.

The invention discloses a brewing process of flavored peach fruit wine, and belongs to the field of fruit wine brewing process processing. The brewing process of the invention is as follows: after fresh peaches are subjected to crushing and enzymolysis, a peach juice is obtained by squeezing and filtering, the sugar content of the peach juice is adjusted, the alcohol content of the peach juice isadjusted to 3 degrees, 5 degrees, 7 degrees and 9 degrees respectively before fermentation, then fermentation is performed on the basis, and finally a finished product is obtained by filtering and blending. According to the brewing process of the invention, alcohol is added before fermentation, on one hand, the extraction effect is achieved, the flavor of the fruit wine is improved, the color of the fruit wine is more clear and translucent, and the fruity aroma and wine aroma are more rich and elegant; and on the other hand, the alcohol tolerance of yeast can be improved, and the contaminationof mixed bacteria in the initial stage of fermentation is reduced.

The present invention provides a method for constructing a genetically engineered bacterium from Pseudomonas cepacia, which replaces the lipase in the genome of Pseudomonas cepacia G63 and a chaperone gene with T7 RNA polymerase genes thereof, so that the T7 RNA polymerase genes are integrated into the genome of Pseudomonas cepacia and controlled by a lipase promoter. Afterwards, the Pseudomonas cepacia lipase and the chaperone gene thereof are cloned into expression plasmid containing T7 promoters and LacI repressor protein, and finally, the plasmid is delivered into the recombinant bacterium of Pseudomonas cepacia to obtain the super engineered bacterium of the Pseudomonas cepacia lipase. The method genetically modifies Pseudomonas cepacia G63, and the obtained super engineered bacterium has high expression and catalysis activity and good low-carbon alcohol tolerance and high-temperature resistance.

The invention discloses an ethanol-resistant Lactobacillus plantarum and its application in fermented food. The Lactobacillus plantarum XH-1 of the present invention is classified and named Lactobacillus plantarum, and is preserved in the General Microorganism Center of the China Microorganism Culture Collection Administration Committee, preservation date: August 16, 2019, preservation address: Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, the preservation number is CGMCC NO.18390. The ethanol-resistant Lactobacillus plantarum of the present invention has an alcohol tolerance of up to 17% (V / V), and has the characteristics of high-yield γ-aminobutyric acid, and the ability to produce γ-aminobutyric acid in the MRS medium reaches 170 g / l, and apply it to wine and vinegar brewing production. The strain can grow and reproduce normally in the high-concentration ethanol environment brewed by wine and vinegar, and can produce high γ-aminobutyric acid. The production process is controllable, and the operation is simple and convenient; The content of phenylethyl alcohol can greatly improve the sensory quality of finished wine and vinegar.

The invention discloses a method for finding biomarkers relevant to tolerance to ethyl alcohol of photosynthetic cyanobacteria. The method comprises the following steps: (1) carrying out ethyl alcohol treatment culture on cyanobacteria; (2) extracting metabolin; (3) performing GC-MS (Gas Chromatography-Mass Spectrometer) detection and data treatment; and (4) carrying out principal component analysis. The method can rapidly find out the biomarkers of the cyanobacteria in an ethyl alcohol stress environment; the biomarkers can provide targets for metabolism path research of ethyl alcohol tolerance of the cyanobacteria, so as to further provide fundamental basis for improving ethyl alcohol tolerance of the cyanobacteria; and the method also can provide a new thinking and a method for optimization of the ethyl alcohol tolerances of other strains.

The invention relates to a gluconobacter oxydans strain which is high in non-volatile organic acid content and capable of tolerating high temperature and high alcohol, separated and screened from figs, and the preservation number of the strain is CGMCC NO: 18500. The gluconobacter oxydans W-1 strain is separated and screened out, the strain has high non-volatile organic acid yield, good high-temperature tolerance and high-alcohol tolerance, high acid yield and good vinegar brewing flavor, the vinegar production efficiency can be improved, the production cost can be saved, high-quality vinegarcan be produced, and the economic benefits of enterprises can be remarkably improved.

The invention discloses a kudzu flower and ginseng compound anti-alcohol and liver-protecting oral liquid and a preparation method thereof. The kudzu flower and ginseng are used as the monarch medicine and supplemented with six traditional Chinese medicines of ganoderma lucidum, chrysanthemum, skullcap, astragalus, mulberry and thyme. The effective essence of traditional Chinese medicine is extracted by CO2 supercritical, and the extract powder is dried and sieved, and then 9% of the extract powder, 15% of jujube honey, 26% of fresh lotus root juice, and 50% of purified water are mixed and stirred to make an oral liquid. Taking it in time before and after drinking can enhance the function of the liver enzyme system, accelerate the decomposition of alcohol, and improve alcohol tolerance to prevent drunkenness and sobering up. Effectively relieves headache, dizziness, polydipsia and vomiting caused by alcoholism, protects and repairs liver cell damage caused by alcoholism; daily use can also nourish and protect the liver, and remove alcohol poisoning accumulated in the body for many years; it is simple and convenient to take, without side effects; and The curative effect is remarkable, the effect is quick, the course of treatment is short, and the total effective rate reaches 100%.

Provided herein is an isolated polynucleotide for increasing the alcohol tolerance of a host cell. Also disclosed herein are a vector and a host cell containing the isolated polynucleotide, and a method of increasing the volumetric productivity of a bioalcohol using the same.

The invention relates to a high-performance environmentally friendly waterborne single-component finish paint used on a plastic substrate and a production process. The high-performance environmentallyfriendly waterborne single-component finish paint used on the plastic substrate comprises the following components in parts by weight: 60-80 parts of waterborne single-component modified acrylic emulsion, 10-20 parts of deionized water, 5-10 parts of a waterborne film-forming aid, 5-10 parts of waterborne celluloses, 0.3-0.5 part of a waterborne leveling agent and 0.1-0.2 part of a waterborne defoaming agent. The high-performance environmentally friendly waterborne single-component finish paint used on the plastic substrate has the beneficial effects that silver arrangement is good, and the advantage of good directionality of waterborne acrylic emulsion with large molecular weight is adopted. Alcohol resistance is good, the waterborne single-component emulsion with the vitrification temperature being 90 or above is adopted, the waterborne single-component emulsion is combined to nitrogen-containing fibers, and the alcohol tolerance is greatly improved. The scratching resistance is high, the vitrification temperature is high, the self-hardness of the resin with the high vitrification temperature is high, and by use of a large amount of directional wax slurry and wax powder with extinction capability, the use amount of digestion powder is greatly reduced, and therefore, the scratching resistance is qualitatively improved.

The invention discloses a liver-protecting health-care molecular wine containing total flavonoids. Traditional processing or soaking methods of traditional Chinese medicine prescriptions are broken through, medicinal and edible homologous plants containing rich flavonoids are enriched and extracted by an enzymatic extraction process to obtain a total flavonoid crude extract fermentation broth, then total flavonoid powder is obtained, and the total flavonoid powder and baijiu are prepared in proportion to obtain molecular wine. The molecular wine has the auxiliary treatment or prevention effect on alcoholic liver injury, can reduce the content of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver tissue supernatant, and increase the total antioxidant capacity (T-AOC) in the liver tissue supernatant, so that the livers are prevented from injuries or the injuries caused by ethyl alcohol are decreased when people enjoy drinking pleasure. Compared with traditionalbrewed wine, the wine has a light color, no extraneous odor and no precipitation impurities, can improve the alcohol resisting ability and alcohol tolerance of drinkers through long-term drinking.

The invention relates to a Lactobacillus plantarum with excellent alcohol tolerance. The name of the lactobacillus plantarum is LTJ12, the classification name is lactobacillus plantarum, the preservation number is CGMCC No.20086, the preservation date is June 15, 2020 in No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation unit is China General Microbiological CultureCollection Center. The lactobacillus plantarum disclosed by the invention is derived from fermented grains in the fermentation process of traditional fermented food white spirit, and belongs to a universally accepted safe strain which can be used for food. The lactobacillus plantarum provided by the invention has excellent alcohol tolerance, and the strain is a dominant strain in fermented grainsof white spirit and has very strong alcohol tolerance.

An in situ method of preparing modified polyvinyl acetals is described with the aim of improving the alcohol tolerance while retaining barrier properties of the base polymer.

The invention discloses a compound type fuel cell cathode catalyst NGPC / NCNTs and a preparation method thereof, belongs to the technical field of catalyst preparation and aims to develop a cheap and efficient non-precious metal catalyst to solve the problem of high cost of a fuel cell cathode catalyst made of a Pt-based material. An MOF (metal-organic framework) material as a C source of the NGPC / NCNTs is pre-carbonized at a low temperature firstly, a graphitization catalyst and a N source are introduced step by step, two times of corresponding high-temperature heat treatment are performed, and the NGPC / NCNTs are prepared. The active component of the NGPC / NCNTs is N-doped graphitized porous C or CNTs, a pore structure is in a micropore-mesopore compound type, and a continuous conduction framework in three-dimensional space is formed through structural connection of dispersed porous C particles by the CNTs, so that the NGPC / NCNTs have good conductivity and higher mass transfer rate and can show efficient catalytic activity, methyl alcohol tolerance and cycle stability, which are better than those of a commercial Pt / C electrocatalyst, in an alkaline medium.