Specific primer pair and probe for detecting ALDH2 (alcohol dehydrogenase 2) gene chip

A specific, primer pair technology, applied in the field of molecular biology, can solve the problems of inaccurate detection results, difficult to meet clinical tests, long detection cycle, etc., achieve good signal-to-noise ratio, avoid uncertain factors, and simple steps.
CN103540675AInactive Publication Date: 2014-01-29SHANGHAI BAIO TECH
5 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI BAIO TECH
Publication Date
2014-01-29
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to the field of molecular biology, and discloses a specific oligonucleotide probe for detecting an SNP (single nucleotide polymorphism) site of ALDH2 (alcohol dehydrogenase 2)gene, wherein the probe is capable of hybridizing with the 504th different genetype of the ALDH2 gene. The invention further discloses a specific primer pair for amplifying the ALDH2 gene when detecting the SNP site, and the primer pair can be used for specifically amplifying a target area containing a detection target site in the ALDH2 gene. The specific oligonucleotide probe and the specific primer pair provided by the invention can be used for detecting the ALDH2 genetype and providing information for people to learn individual alcohol metabolism detoxification ability and correctly use nitroglycerin.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the fields of molecular biology and nucleic acid detection, and specifically designs a pair of specific primers for amplifying the ALDH2 gene and a specific probe for detecting the ALDH2 gene chip. Background technique

[0002] Human acetaldehyde dehydrogenase (ALDH) is a tetrameric protein that catalyzes the oxidation of acetaldehyde and other aliphatic aldehydes. There is a single nucleotide polymorphism (single nucleotide polymorphism, SNP) in exon 12 of the ALDH2 gene—rs671, and a base substitution occurs: guanine (G) → adenine (A), which leads to a codon change: GAA→AAA, and then the 504th amino acid of the enzyme changed, glutamic acid (Glu)→lysine (Lys). There are two alleles of ALDH2: wild-type (ALDH2*1, *504Glu), mutant (ALDH2*2, *504Lys). Studies have confirmed that the mutation of ALDH2 leads to significant changes in enzyme activity, which will seriously affect the metabolism of alcohol, nitroglycerin, etc., and t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More