Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer group and kit for detecting genetic typing of human aldehyde dehydrogenase 2 (ALDH2)

A technology of aldehyde dehydrogenase and genotyping, which is applied in the field of kits for detecting ALDH2 genotyping, and can solve problems such as ineffective effects

Inactive Publication Date: 2013-10-23
天津市秀鹏生物技术开发有限公司
View PDF8 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people saved their lives in time because of it, while some people took it, but the effect was not very obvious

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer group and kit for detecting genetic typing of human aldehyde dehydrogenase 2 (ALDH2)
  • Primer group and kit for detecting genetic typing of human aldehyde dehydrogenase 2 (ALDH2)
  • Primer group and kit for detecting genetic typing of human aldehyde dehydrogenase 2 (ALDH2)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] According to the ALDH2 allele sequence released by the National Center for Biotechnology Information Gene Bank (NCBI Gene Bank), a primer set for detecting ALDH2 genotyping was designed. The primer set includes ALDH2*1 primer pair, ALDH2*2 primer pair and internal reference primers.

Embodiment 2

[0024] The preparation method of the kit for detecting ALDH2 genotyping is:

[0025] (1) According to the well location map of ALDH2 gene-specific primers (see figure 1 ), respectively coating the ALDH2*1 primer pair, the ALDH2*2 primer pair and the internal reference primer pair on the corresponding positions of the primer plate, and drying them;

[0026] (2) Prepare 440 μl concentrated dNTP-Buffer according to the combination of 200mM dNTP, 3.5mM Mg2+, 500mM KCL, 100mM Tris-HCL, and 1% TritonX-100;

[0027] (3) The primer plate coated with the primer pair includes 2 wells of specific primer wells per person, and the dNTP-Buffer is concentrated to form a kit for human ALDH2 genotyping detection.

Embodiment 3

[0029] Use the operation process

[0030] 1. Preparation of 2.5% agarose gel:

[0031] 1. Add 2.5g of agarose to 100ml of 1×TBE solution (Tris-borate-EDTA solution) and heat until a uniform gel solution is formed. Then add an appropriate amount of electrophoresis dye and mix well.

[0032] 2. Put the gel tank in a horizontal position, add an appropriate amount of gel solution into the gel tank, and insert the electrophoresis comb. Move the gel tank horizontally back and forth to ensure even coverage of the gel solution.

[0033] 3. After the gel is completely solidified, pull out the electrophoresis comb vertically.

[0034] 2. PCR cycle parameters are shown in Table 1:

[0035] 1

96℃ / 2min

1cycle

2

96°C / 20sec, 68°C / 60sec

5cycles

3

96°C / 20sec, 65°C / 50sec, 72°C / 45sec

10cycles

4

96°C / 20sec, 63°C / 50sec, 72°C / 45sec

15cycles

5

72℃ / 3min

1cycle

6

Store at 4°C

[0036] Table 1 Ampl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a primer and a kit for detecting genetic typing of human aldehyde dehydrogenase 2 (ALDH2). The primer group for detecting human ALDH2 gene typing comprises an ALDH2*1 primer pair, an ALDH2*2 primer pair and an internal reference primer pair. The application of the kit helps to realize good typing for ALDH2 gene, and has reference value for hereditary dangerous factors such as drinking behavior, alcoholic cirrhosis, liver cancer, nasopharyngeal carcinoma, oral cancer and the like, and glyceryl trinitrate nitroglycerin guiding pharmacy.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a kit for detecting ALDH2 genotyping. Background technique [0002] The human acetaldehyde dehydrogenase 2 (ALDH2) gene is located on chromosome 12 (12q24.2), and its main polymorphism is rs671, which is G1510A located in exon 12. The normal allele is denoted as ALDH2*1, and the allele with a single base mutation is denoted as ALDH2*2. The distribution of ALDH2*2 in various human groups is different, and it basically appears in Asians. Studies have shown that the allele frequency of ALDH2*2 in Chinese is 18%, among which the Guangdong Han is the highest (31%), Wuhan Han is 12%, Luoyang is 15%, Shanghai is 25%, and Taiwan is 30%. North Korean 16%, Japanese 27%, Thai 4%, white 0%, black 0%. [0003] Acetaldehyde dehydrogenase is a tetramer of random combination, and a mutant subunit affects the stability of the tetramer, thereby affecting the normal expression of the enzyme. ALDH2*...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 赵卫军
Owner 天津市秀鹏生物技术开发有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com