Gene detection reagent kit for evaluating alcohol tolerance

Abstract

The invention provides a gene detection reagent kit for evaluating alcohol tolerance. The gene detection reagent kit comprises six amplification primers for three genes including an internal reference gene GAPDH (glyceraldehyde phosphate dehydrogenase), a gene ADH1B (ethanol dehydrogenase 1B) and a gene ALDH2 (acetaldehyde dehydrogenase 2) and five Taq-man probe primers. One of the Taq-man probe primers is positioned at the internal reference gene GAPDH and is used for carrying out FAM fluorescence labeling; two other Taq-man probe primers are SNP (single nucleotide polymorphism) probes positioned at the gene ADH1B and are used for carrying out VIC and ROX (roxithromycin) labeling; the remaining two Taq-man probe primers are SNP probes positioned at the gene ALDH2 and are used for Cy5 and Quasar705 labeling, BHQ is used as a quenching group, and delta ct values can be computed. The gene detection reagent kit has the advantages that the alcohol tolerance of healthy persons can be evaluated by the gene detection reagent kit from the aspect of genes, and accordingly the healthy persons can know the strength and the weakness of alcohol tolerance, can drink alcohol scientifically and can carry on occasion such as party, gathering and banquet related to alcohol culture.

Description

Technical field [0001] The invention relates to the field of molecular biology and laboratory medicine, and is mainly used in health examinations, occupations related to drinking, such as wine tasters, and is also closely related to areas such as daily life, holiday parties and other drinking activities, to evaluate personal tolerance to alcohol ability. Background technique [0002] As a long tradition of the Chinese nation, wine culture has profound connotation and heritage. Drinking is something that everyone cannot avoid in daily life, work, and interpersonal communication. Some people seem to be born with the physique of drinking, not drunk for a thousand cups, which is astounding; while some people pour just one cup, blush and even have alcoholism. At present, there are few reports on the evaluation and level of drinking ability, or just stay on how to ensure a certain amount of drinking to reduce the harm of alcohol to the body. However, with the development of biology, ...

AI Technical Summary

Problems solved by technology

[0005] However, there are few reports on the systematic evaluation of drinking ability by detecting the combination of these two SNP sites, and the cost of sequencing is high. Most PCR methods need to amplify the two sites separately, which is time-consuming and laborious, and only The ability to degrade ethanol from these two mutation sites lacks scientific rigor, and the changes in genes and the level of alcohol consumption in daily life have not been well studied and related, and there is no substantive and scientific basis for people's alcohol consumption. Sexual Guidance Advice

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