22 results about "Mutant phenotype" patented technology

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The invention discloses application of an NLD gene in regulation and control of an included angle of corn leaves. According to the invention, a corn mutant el20 with a reduced leaf angle is obtained through mutant screening, and a gene NLD causing a mutant phenotype is positioned through a map-based cloning technology. The overexpression of the NLD gene can restore the leaf upright phenotype of el20. The NLD gene is edited by using a gene editing technology, so that rapid creation of the compact plant type corn is realized. The invention provides a novel method and a molecular module for cultivating compact plant type corn suitable for close planting.

The invention discloses a corn kernel development regulation gene ZmRH51 as well as an encoding protein, a functional marker and an application of the corn kernel development regulation gene ZmRH51. According to the application, a corn kernel mutant m223 is taken as a basic material, heterozygote of the corn kernel mutant m223 is hybridized with Zheng 58 (Z58) to construct an F2 segregation population, then phenotype identification and cytological observation are performed on the segregation population of the offspring, the gene ZmRH51 for controlling the mutation phenotype of the m223 is obtained through genetic analysis, BSR-seq analysis and map-based cloning, the gene is positioned in a nucleolus region, and the mutation phenotype of the m223 is controlled. The ZmRH51 is used for coding RNA helicase of a DEAD-Box gene family, and research shows that ZmRH51 participates in ribosome assembly and can influence processing of pre-rRNA in corn kernels; the excellent allelic variation genotype of the excavated ZmRH51 gene in the aspect of grain thickness is obtained through further excavation, the grain thickness and hundred-grain weight of the ZmRH51 gene can be increased through the utilization of the gene, and then the yield is increased. In addition, distribution and utilization potentials of different haplotypes in a selfing line are researched, and technical support and excellent germplasm resources are provided for utilization of the gene.

The application relates to a mycobacterium tuberculosis drug resistance phenotype prediction method and device and a computer device. The method comprises the following steps: determining a to-be-predicted mutation site which does not exist in a mutation phenotype annotation database from at least one mutation site of a target gene fragment of to-be-processed mycobacterium tuberculosis according to gene sequencing data of the mycobacterium tuberculosis; converting protein structure change data of the to-be-predicted mutation site according to protein structure data corresponding to the to-be-predicted mutation site; inputting the protein structure change data of the to-be-predicted mutation site into a drug resistance phenotype prediction model to obtain a predicted drug resistance phenotype of the to-be-predicted mutation site; and determining a drug resistance phenotype prediction result of the to-be-processed mycobacterium tuberculosis based on the predicted drug resistance phenotype of the to-be-predicted mutation site and drug resistance phenotypes of the mutation sites except the to-be-predicted mutation site. The method can effectively improve the mycobacterium tuberculosis drug resistance phenotype prediction efficiency.

The invention provides an SNP (Single Nucleotide Polymorphism) marker of an Rh blood group system and application of the SNP marker in detecting RhD variation phenotypes. RhD variation blood groups of red blood cells can be confirmed by detecting an SNP site. The SNP marker is located at the 121st basic group of a nucleotide fragment of SEQ ID NO: 1, that is, the 922nd basic group from an initiation codon in an RHD gene coding region is mutated, and G is mutated into C. The SNP marker of an Rh blood group system is obtained through detection and screening, and whether an individual to be detected has an RhD variation phenotype or not is determined by detecting whether the SNP marker exists or not. The application effect shows that the SNP locus and the detection primer provided by the invention can be effectively used for rapid detection of the RHD gene of clinical patients and blood station blood donors.

The present invention provides methods for determining the phenotype (e.g., gain-of-function or loss-of-function) of a mutation in an ion channel or receptor by using a dynamic voltage clamp. The invention also features methods of determining whether a mutation is a gain-of-function or loss- of-function mutation and treating a disease or disorder associated with the particular gain-of-function or loss-of-function mutation.

This invention application discloses a gene regulating maize kernel development. ZmRH51 This application focuses on the encoded proteins, functional markers, and applications of maize kernel mutants. m223 Using this as the basic material, its heterozygotes were crossed with Zheng 58 (Z58) to construct the F2 segregating population. Phenotypic identification and cytological observation were performed on the progeny segregating population. Controlling populations were obtained through genetic analysis, BSR-seq analysis, and map-based cloning. m223 Genes with mutant phenotypes ZmRH51 This gene is located in the nucleolar region and encodes an RNA helicase belonging to the DEAD-Box gene family. Studies have shown that... ZmRH51 It participates in ribosome assembly and can influence the processing of pre-rRNA in maize kernels; and further mining yields... ZmRH51 The superior allelic variant genotype of this gene in terms of grain thickness can be utilized to improve grain thickness and 100-grain weight, thereby increasing yield. Furthermore, the distribution and utilization potential of different haplotypes in inbred lines were studied, providing technical support and excellent germplasm resources for the utilization of this gene.

The invention belongs to the field of rice genetic breeding, and particularly relates to application of a rice calmodulin gene in regulation and control of rice high-temperature stress resistance. The invention discloses application of a rice calmodulin gene in regulation and control of high temperature stress resistance of rice, the nucleotide sequence of the rice calmodulin gene is shown as SEQ ID NO.1, CDS is shown as SEQ ID NO.2, and the amino acid sequence of protein coded by the rice calmodulin gene is shown as SEQ ID NO.3. The invention further discloses a preparation method of the rice calmodulin gene. The calmodulin gene is knocked out through a gene editing technology, the nucleotide sequence of the calmodulin mutant gene is as shown in SEQ ID NO.4, CDS is as shown in SEQ ID NO.5, the amino acid sequence of encoded protein is as shown in SEQ ID NO.6, and the high temperature sensitivity, heat resistance and relative setting rate of the mutant phenotype are remarkably reduced. The invention can provide new gene resources and theoretical basis for high-temperature resistance genetic breeding of rice.

The application discloses a corn size and amino acid content regulating gene ZmCRK3 , its encoded protein, SNP site, its functional marker and application, aiming to provide excellent haplotypes and germplasm resources with excellent haplotypes for improving corn yield and quality. ZmCRK3 The kernel mutant of the gene was identified by genotyping and linkage analysis to be co-segregated with the kernel mutant phenotype. ZmCRK3 The mutation affects the kernel size and amino acid content of corn, and provides primers, amplification systems and PCR reaction procedures for detecting its mutant genotype; through association analysis, SNPs that affect kernel size and amino acid content were discovered, and haplotype analysis clarified ZmCRK3 The excellent haplotypes were identified ZmCRK3 Germplasm resources with excellent haploidy; utilizing the genes and molecular markers disclosed in this application can facilitate the creation of high-yield and high-quality breeding materials for corn, shorten the breeding cycle of new corn varieties, reduce breeding costs, and improve breeding efficiency.

The invention provides a method for visually screening rice mutants and application thereof, and belongs to the technical field of biology. The method for visually screening the rice mutants comprises the following steps: transferring a recombinant expression vector based on an inducible promoter into rice to obtain a transgenic plant, carrying out seed reproduction on the transgenic plant, and then screening to obtain the mutants, the recombinant expression vector based on the inducible promoter comprises a promoter of a candidate gene and a reporter gene, and the screening comprises a step of low-temperature treatment. According to the screening method disclosed by the invention, the time required for screening the rice mutant phenotype is shortened, and meanwhile, the phenotype screening result is more accurate.

The invention discloses a DNA site-directed mutagenesis method and a protein mutant phenotype screening method based on the DNA site-directed mutagenesis method. The DNA site-directed mutagenesis method comprises a first reaction, a second reaction and a third reaction. According to the DNA site-directed mutagenesis method provided by the invention, the experimental process of the PCR part is optimized and improved, the construction of the DNA site-directed mutagenesis can be realized through simple three PCR cycles without DNA purification or monoclonal selection, and the method has high success rate and accuracy.

The present invention discloses a method for site-directed DNA mutagenesis and a method for screening protein mutant phenotypes based thereon. Among them, the method for site-directed DNA mutagenesis includes: a first reaction, a second reaction, and a third reaction. The method for site-directed DNA mutagenesis provided by the present invention optimizes and improves the experimental procedure of the PCR part. Through simple three-round PCR cycles, the construction of site-directed DNA mutagenesis can be achieved without DNA purification or monoclonal picking, and it has a high success rate and accuracy.

The application discloses application of an NLD gene in regulating leaf angle of corn. A corn mutant el20 with small leaf angle is obtained through mutant screening, and a gene NLD causing the mutant phenotype is located through a map-based cloning technique. Overexpression of the NLD gene can restore the leaf straightening phenotype of the el20. Rapid creation of compact corn is realized by editing the NLD gene through a gene editing technique. The application provides a new method and molecular module for breeding compact corn suitable for close planting.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsBC1L6 gene in regulation and control of drought resistance of rice. The OsBC1L6 gene capable of weakening the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsBC1L6 gene is as shown in SEQ ID NO. 1, and the sequence of protein coded by the OsBC1L6 gene is as shown in SEQ ID NO. 2. The rice drought response control gene OsBC1L6 is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, seedling stage drought stress phenotype identification shows that when a gene segment is deleted, the drought stress resistance of rice is enhanced, and the function and the application way of the OsBC1L6 are proved.

This invention discloses the ZmGRAL1 protein, its encoding gene, and its applications, which are related to the stage-specific regulation of maize leaf color phenotype. It belongs to the fields of plant genetic engineering and breeding technology. This invention screened and obtained a maize leaf color mutant. gral1 ,Should gral1 The mutant exhibits a significant albino phenotype before V5, followed by regreening; agronomic traits such as plant height, 100-grain weight, and grain number per ear are not different from the wild type, making it a leaf color mutant with application value. For analysis... gral1 The molecular mechanism of mutant phenotype formation was isolated in this invention using map-based cloning technology. ZmGRAL1 Genes, and verified through gene knockout. ZmGRAL1 Genes that regulate the development of maize leaf color. ZmGRAL1 Genes can regulate chloroplast development and stage-specific regulation of maize leaf phenotype, and can be used as maternal marker genes to efficiently identify false hybrids through leaf color during the seedling stage.

The invention belongs to the technical field of plant genetic engineering, and discloses application of a rice OsORR1 gene in controlling drought resistance of rice. The OsORR1 gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsORR1 gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The candidate gene is subjected to CRISPR mutant phenotype identification, and seedling stress phenotype identification shows that when the gene segment is deleted, the drought resistance of rice is reduced, and the drought resistance of the rice can be remarkably enhanced by overexpression of OsORR1, so that the function and the application way of the gene are proved.

The invention belongs to the technical field of plant genetic engineering, and discloses application of a rice OsHT gene in improvement of drought resistance of rice. Clustered regularly interspaced short palindromic repeats (CRISPR) mutant phenotype identification is carried out on candidate genes, and the OsHT gene for controlling rice drought response is successfully cloned. The drought stress phenotype identification results in the seedling stage and the adult-plant stage show that when the gene segment is deleted, the drought stress resistance of the rice is obviously enhanced, so that the function of the gene and the potential of the gene in practical application are proved.

The invention provides an application of an FveGPA1 gene in regulation and control of strawberry inflorescence branching, a coding sequence of the FveGPA1 gene is shown as SEQ ID NO.1, and an amino acid sequence of protein coded by the FveGPA1 gene is shown as SEQ ID NO.2. The invention further provides an application of the FveGPA1 gene in regulation and control of strawberry inflorescence branching. Researches find that FveGPA1 gene mutation can significantly change an inflorescence branching mode, so that most of original top branching inflorescences are converted into middle branching inflorescences and base branching inflorescences. Besides, the FveGPA1 mutant also shows unique leaf morphological characteristics, including leaf rounding and middle small petiole elongation, so that the mutation phenotype can be accurately identified in the seedling stage, and convenience is provided for early screening. The strawberry inflorescence structure can be adjusted by reasonably utilizing the gene, the uniformity of the size of strawberry fruits can be improved, important theoretical basis and means are provided for strawberry molecular breeding, and the gene has great application value.

The invention discloses an LLC cell mEgfrp.L860R mutant strain Mus musculus and application of the LLC cell mEgfrp.L860R mutant strain Mus musculus, and belongs to the technical field of cell engineering. According to the LLC cell mEgfrp.L860R mutant strain Mus musculus, on the basis of an LLC cell line, by accurately introducing EGFR typical mutation, the clinical state of an EGFR mutant NSCLC patient is successfully simulated. Compared with a traditional EGFR mutant cell model, the LLC cell mEgfrp.L860R mutant strain Mus musculus not only has a stable EGFR mutant phenotype, but also can well reflect the dynamic change of the immune microenvironment of tumor cells in the EGFR-TKI process, provides a powerful in-vitro and in-vivo experimental tool for researching the construction of the tumor immune microenvironment using EGFR-TKI, and has an important clinical transformation value.

The invention provides a shellfish gene editing method taking sperm as a delivery carrier, an exogenous nucleic acid fragment for editing shellfish genes is inserted into a used recombinant plasmid, and a promoter of the method is a promoter with a sequence of SEQ ID NO: 1. According to the method disclosed by the invention, the CRISPR / Cas9 recombinant expression plasmid can be successfully introduced into the shellfish embryo. After artificial insemination is carried out according to the sperm-egg ratio of 1500: 1, the fertilization rate and the hatching rate are kept at the high level and reach 95% and 80% respectively. In the D-type larva stage of the dwarf, the plasmid introduction rate can reach 35%-45%, and adult individuals carrying the target mutation phenotype have been successfully screened out. Compared with the prior art, the method using the sperms as the delivery carrier has the advantages of being easy and convenient to operate, low in cost, short in consumed time and the like, large-scale operation is easy to achieve, the biological characteristic that the shellfish egg laying amount is large can be well adapted, and the actual breeding and production requirements are met.

The invention discloses a trichogramma gene editing method. The method comprises the following steps: (1) preparing a test bee species and hosts: preparing host tussah eggs; trichogramma is inoculated, and the trichogramma is inoculated for 6-8 hours; the parasitized tussah eggs are clamped in a fruit fly tube to be cultured; (2) preparation of pupae for injection: culturing in an incubator for 9-10 days, and splitting tussah eggs to obtain female pre-pupae of trichogramma; (3) preparing a solution for injection: mixing Cas9 and sgRNA, and incubating to form an RNP compound; (4) micro-injection: injecting the solution into hemolymph; (5) culturing female pupae in vitro after injection; (6) sub-packaging single heads of the eclosion female bees into finger-shaped tubes, and putting corcyra cephalonica egg cards for parasitizing until the offspring drones are all eclosion; and (7) screening mutants: observing mutation phenotypes under a stereoscope, and determining that gene editing succeeds through PCR (Polymerase Chain Reaction) detection. According to the method, the hosts are replaced by the tussah eggs from the rice moth eggs, so that mechanical damage caused in the dissecting process is reduced.

The application belongs to the technical field of plant genetic engineering, and discloses DRG9 Application of a gene in regulating high-temperature resistance of rice. DRG9 The application obtains a gene capable of improving high-temperature tolerance of rice through separation, cloning and function verification. DRG9 The protein sequence coded by the gene is shown as SEQ ID.2. The application clones a high-temperature response gene of rice DRG9 , and performs CRISPR mutant phenotype identification on the candidate gene. Through high-temperature stress phenotype identification in the seedling stage and the adult stage, it is shown that when the gene fragment is deleted, the high-temperature stress tolerance of rice is reduced, and when the gene is overexpressed, the high-temperature tolerance is improved, thereby confirming the function and application approach of the gene.