7 results about "Mutant phenotype" patented technology

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The application relates to a mycobacterium tuberculosis drug resistance phenotype prediction method and device and a computer device. The method comprises the following steps: determining a to-be-predicted mutation site which does not exist in a mutation phenotype annotation database from at least one mutation site of a target gene fragment of to-be-processed mycobacterium tuberculosis according to gene sequencing data of the mycobacterium tuberculosis; converting protein structure change data of the to-be-predicted mutation site according to protein structure data corresponding to the to-be-predicted mutation site; inputting the protein structure change data of the to-be-predicted mutation site into a drug resistance phenotype prediction model to obtain a predicted drug resistance phenotype of the to-be-predicted mutation site; and determining a drug resistance phenotype prediction result of the to-be-processed mycobacterium tuberculosis based on the predicted drug resistance phenotype of the to-be-predicted mutation site and drug resistance phenotypes of the mutation sites except the to-be-predicted mutation site. The method can effectively improve the mycobacterium tuberculosis drug resistance phenotype prediction efficiency.

The invention provides a method for visually screening rice mutants and application thereof, and belongs to the technical field of biology. The method for visually screening the rice mutants comprises the following steps: transferring a recombinant expression vector based on an inducible promoter into rice to obtain a transgenic plant, carrying out seed reproduction on the transgenic plant, and then screening to obtain the mutants, the recombinant expression vector based on the inducible promoter comprises a promoter of a candidate gene and a reporter gene, and the screening comprises a step of low-temperature treatment. According to the screening method disclosed by the invention, the time required for screening the rice mutant phenotype is shortened, and meanwhile, the phenotype screening result is more accurate.

This invention discloses the ZmGRAL1 protein, its encoding gene, and its applications, which are related to the stage-specific regulation of maize leaf color phenotype. It belongs to the fields of plant genetic engineering and breeding technology. This invention screened and obtained a maize leaf color mutant. gral1 ,Should gral1 The mutant exhibits a significant albino phenotype before V5, followed by regreening; agronomic traits such as plant height, 100-grain weight, and grain number per ear are not different from the wild type, making it a leaf color mutant with application value. For analysis... gral1 The molecular mechanism of mutant phenotype formation was isolated in this invention using map-based cloning technology. ZmGRAL1 Genes, and verified through gene knockout. ZmGRAL1 Genes that regulate the development of maize leaf color. ZmGRAL1 Genes can regulate chloroplast development and stage-specific regulation of maize leaf phenotype, and can be used as maternal marker genes to efficiently identify false hybrids through leaf color during the seedling stage.

The invention discloses an LLC cell mEgfrp.L860R mutant strain Mus musculus and application of the LLC cell mEgfrp.L860R mutant strain Mus musculus, and belongs to the technical field of cell engineering. According to the LLC cell mEgfrp.L860R mutant strain Mus musculus, on the basis of an LLC cell line, by accurately introducing EGFR typical mutation, the clinical state of an EGFR mutant NSCLC patient is successfully simulated. Compared with a traditional EGFR mutant cell model, the LLC cell mEgfrp.L860R mutant strain Mus musculus not only has a stable EGFR mutant phenotype, but also can well reflect the dynamic change of the immune microenvironment of tumor cells in the EGFR-TKI process, provides a powerful in-vitro and in-vivo experimental tool for researching the construction of the tumor immune microenvironment using EGFR-TKI, and has an important clinical transformation value.

The invention provides a shellfish gene editing method taking sperm as a delivery carrier, an exogenous nucleic acid fragment for editing shellfish genes is inserted into a used recombinant plasmid, and a promoter of the method is a promoter with a sequence of SEQ ID NO: 1. According to the method disclosed by the invention, the CRISPR / Cas9 recombinant expression plasmid can be successfully introduced into the shellfish embryo. After artificial insemination is carried out according to the sperm-egg ratio of 1500: 1, the fertilization rate and the hatching rate are kept at the high level and reach 95% and 80% respectively. In the D-type larva stage of the dwarf, the plasmid introduction rate can reach 35%-45%, and adult individuals carrying the target mutation phenotype have been successfully screened out. Compared with the prior art, the method using the sperms as the delivery carrier has the advantages of being easy and convenient to operate, low in cost, short in consumed time and the like, large-scale operation is easy to achieve, the biological characteristic that the shellfish egg laying amount is large can be well adapted, and the actual breeding and production requirements are met.

The application belongs to the technical field of plant genetic engineering, and discloses DRG9 Application of a gene in regulating high-temperature resistance of rice. DRG9 The application obtains a gene capable of improving high-temperature tolerance of rice through separation, cloning and function verification. DRG9 The protein sequence coded by the gene is shown as SEQ ID.2. The application clones a high-temperature response gene of rice DRG9 , and performs CRISPR mutant phenotype identification on the candidate gene. Through high-temperature stress phenotype identification in the seedling stage and the adult stage, it is shown that when the gene fragment is deleted, the high-temperature stress tolerance of rice is reduced, and when the gene is overexpressed, the high-temperature tolerance is improved, thereby confirming the function and application approach of the gene.